6 results about "Host response" patented technology

Systems and methods for the detection of sepsis and treatment of patients. A sepsis detection system includes a first subsystem configured to detect the presence of an infection in a patient, a second subsystem configured to detect the presence of a dysregulated immune response in the patient, a third subsystem configured to detect organ dysfunction in the patient, a fourth subsystem configured to detect antibiotic resistance (ARB) of a pathogen in the patient, and a processing device. The first, second, third, and fourth subsystems and the processing device are communicatively coupled to each other via a network. The processing device is configured to determine the presence of sepsis in the patient based on the presence of infection, the presence of the dysregulated host response, and clinical data indicative of organ dysfunction in the patient.The subsystems utilize at least one polymerase chain reaction (PCR) procedure, Raman spectroscopy, clinical data, electronic health record (EHR) data, and antibiotic susceptibility testing (antibiogram).

This invention relates to the fields of environmental monitoring and biosafety technology, and in particular to a viral aerosol sampling patch for face masks, comprising: a capture layer for intercepting viral aerosol particles in the air; a culture layer disposed inside the capture layer, the culture layer comprising a culture unit supporting the virus to maintain activity and replicate in the presence of a host, a host bacterium sensitive to the virus, and a visual indicator indicating viral replication or host response; and a base layer disposed inside the culture layer; the sampling patch is fixed to the face mask through the base layer, and viral aerosol collection is achieved without external power through the capture layer, and countable detection results are generated after sampling through the culture layer; utilizing the airflow and humidity conditions naturally formed during wearing, effective interception, activity preservation, and visual detection of viral aerosols are achieved without complex equipment and additional operating steps, and the operation is simple and repeatable.

This invention discloses a mass spectrometry patch for wound infection detection and its application, relating to the field of biomedical device technology. The mass spectrometry patch consists of a contact layer, a collection layer, and a protective layer from bottom to top. The contact layer is a polycarbonate mesh film, the collection layer is a polyvinylidene fluoride nanofiber membrane with high-density gold nanoparticles grown in situ on its surface, and the protective layer is a polyurethane film. This invention provides a wearable mass spectrometry patch integrating sampling and detection, achieving non-invasive sampling and direct mass spectrometry analysis. A single detection using laser desorption / ionization mass spectrometry can obtain a global metabolic fingerprint containing hundreds of metabolites, which simultaneously contains pathogen species-specific signals and host metabolic biomarker signals. By training an AI model to analyze the complex fingerprint, rapid pathogen identification and dynamic monitoring of host responses (such as lactic acid and glucose) can be completed simultaneously within 5 minutes, achieving decoupling of pathogen information from changes in host biomarkers.

The invention discloses a method and a system for synchronously detecting intracellular microbial colonization and host response at a single cell level, and belongs to the technical field of single cell epimics and microbiomics. Microorganism colonization information and host chromatin openness information of each single cell are synchronously extracted from single cell chromatin accessibility sequencing data, and correlation analysis is performed on the microorganism colonization information and the host chromatin openness information at the single cell level, so that a cell specificity mechanism of host-microorganism interaction is analyzed. At present, a systematic method capable of synchronously realizing microbiological detection and host chromatin accessibility analysis under single cell resolution is not reported yet, and the method fills the technical blank. The method can solve the problem that the existing sequencing technology based on 16S rRNA, 18S rRNA or scRNA-seq is difficult to analyze microorganism colonization and host chromatin accessibility synchronously and highly sensitively at the single cell level, and provides an innovative tool for revealing a host-microorganism interaction mechanism of cell specificity in complex tissues.

This invention relates to a method for screening multi-omics features of rabbit cecum for judging the effectiveness of antibiotic-free feeding. Cecum samples are collected from rabbits in antibiotic-free and control feeding groups to obtain microbiome, metabolome, and host response omics data. Candidate microbiome features, candidate metabolite features, and candidate host response features are screened based on inter-group differences. Closed-loop matching of the candidate features is performed according to preset correspondence rules to obtain closed feature groups, and candidate features that do not form closed feature groups are eliminated. Then, based on the inter-group specificity and intra-group consistency of the closed feature groups between the antibiotic-free and control feeding groups, multi-omics features of rabbit cecum for judging the effectiveness of antibiotic-free feeding are obtained. This invention significantly improves the accuracy, stability, and biological interpretability of judging the effectiveness of antibiotic-free feeding by constructing closed feature groups and combining uniqueness, breakpoints, and host permission gating screening.

Provided are gene editing systems and methods for reducing cell immunogenicity and reducing graft versus host (GvH) response, or to promote the persistence of therapeutic cells. Also provided are polynucleotides, vectors, cells, kits and compositions comprising components of the gene editing systems, and methods related to treatment for reducing cell immunogenicity and reducing GvH response.