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Methods of inducing cell differentiation with placental extracts

a placental extract and cell technology, applied in the field of cancer therapy, to achieve the effect of reducing proliferation

Inactive Publication Date: 2011-03-24
MEDISTEM LAB
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

"The present invention describes methods for generating compositions that can treat cancer patients using stem cells. These stem cells can be administered to patients to induce differentiation and reduce the side effects of traditional cancer treatments. The invention also includes a method of inducing differentiation using a combination of stem cells and low molecular weight fractions from cord blood stem cells. The stem cells can be purified and concentrated using solid phase adsorption or other methods. The invention can also be used to increase the immunogenicity of tumor tissue and sensitize cancer cells to immune therapy. The placental tissue can be obtained from healthy mothers and can be homogenized, purified, and administered to patients. Overall, the invention provides new methods for generating therapeutic compositions for cancer treatment."

Problems solved by technology

The use of cellular differentiation therapy has been previous attempted in cancer patients; however, the majority of such differentiation inducers have been synthetic chemicals administered at unnaturally high doses.

Method used

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  • Methods of inducing cell differentiation with placental extracts
  • Methods of inducing cell differentiation with placental extracts
  • Methods of inducing cell differentiation with placental extracts

Examples

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examples

[0052]The following examples are offered to illustrate, but not to limit, the claimed invention.

1. CD34+ Cell Purification

[0053]Cord blood was obtained from consenting mothers undergoing cesarean delivery of healthy, full-term infants, and low-density (less than 1.077 g / ml) cells were isolated by centrifugation on Ficoll-Hypaque. CD34+ cell-enriched populations (65-98% CD34+ cells) were obtained by removal of lineage marker-positive cells using a column (n=2); and positive (EasySep) selection using magnetic beads (n=1; StemCell Technologies Inc.). Cells were stimulated overnight for in vivo experiments and for 48 hours for in vitro experiments at densities less than or equal to 2×105 cells / ml in Iscove's medium supplemented with 1% BSA, 10 μg / ml bovine pancreatic insulin, and 200 μg / ml human transferrin (BIT; StemCell Technologies Inc.), 10−4 mol 2-mercaptoethanol, 2 mM glutamine, 100 ng / ml FL (Immunex Corp.), 100 ng / ml SF, 50 ng / ml Tpo (Genentech Inc.), and 100 ng / ml hIL-6 (provide...

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Abstract

Disclosed are methods of preparing and using placentally-derived stem cells and compositions useful for the treatment of cancer. Said stem cells and compositions function through inducing a “guided differentiation” program in cancer cells, thereby reducing malignancy. Further extension of the invention pertains to augmenting ability of administered cells to induce differentiation through the co-administration of known differentiation inducing agents. Within the context of this disclosure, methods for inducing host responses to cancer are also described.

Description

CROSS-REFERENCE TO RELATED APPLICATIONS[0001]This application is a continuation of U.S. application Ser. No. 11 / 486,635, entitled METHODS OF INDUCING CELL DIFFERENTIATION WITH PLACENTAL EXTRACTS, which was filed on Jul. 13, 2006, which claimed priority under 35 U.S.C. §119 to U.S. Provisional Application No. 60 / 699,579, which was filed on Jul. 14, 2005, the disclosure of each of which is hereby incorporated by reference in its entirety.BACKGROUND[0002]1. Field of the Invention[0003]The invention disclosed relates generally to the fields of cancer therapy. More particularly, it relates to the process of cancer cell differentiation into cells of reduced malignancy, or lacking of malignancy. More specifically, the invention pertains to the utilization of cord-blood and placentally derived stem cells for the stimulation of cancer cell differentiation.[0004]2. Description of Related Art[0005]In the field of cellular biology, the definition of “stem cell” is dependent on function more tha...

Claims

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Application Information

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Patent Type & Authority Applications(United States)
IPC IPC(8): A61K35/50C12N5/073A61P35/04A61K35/44
CPCA61K35/44C12N5/0605A61K35/50A61P35/00A61P35/04C12N5/0694
Inventor ICHIM, THOMAS E.
Owner MEDISTEM LAB
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