IgG antibody detection kit of TORCH five items and preparation of kit

A detection kit and antibody detection technology, applied to measuring devices, instruments, scientific instruments, etc., to achieve high sensitivity, good specificity, and low cost

Inactive Publication Date: 2013-04-03
BEIJING KINGHAWK PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0011] At present, there is no report on the combined detection of TOCRH five IgM or IgG antibodies using strip immunoblotting technology. After research, the present invention uses strip immunoblotting to measure TOCRH five IgM or IgG antibodies with high accuracy and high sensitivity. , simple operation, no interference, cheap price

Method used

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  • IgG antibody detection kit of TORCH five items and preparation of kit
  • IgG antibody detection kit of TORCH five items and preparation of kit
  • IgG antibody detection kit of TORCH five items and preparation of kit

Examples

Experimental program
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Effect test

Embodiment 1

[0061] 1. Preparation of detection reagent strips:

[0062] Such as figure 1 Display: TORCH five-item IgG antibody (strip immunoblotting) detection kit consists of: PVC carrier plate, PVDF membrane, Toxoplasma gondii (TOX) cleavage antigen and specific recombinant antigen, rubella virus (RV) cleavage antigen And specific recombinant antigen, cytomegalovirus (CMV) split antigen and specific recombinant antigen, herpes simplex virus type 1 and type 2 (HSV-I / II) split antigen and specific recombinant antigen, anti-human IgG and other TORCH raw materials It is composed of 9 coating lines made of coating solution.

[0063] Using 0.01M pH7.2-7.4 phosphate buffer (PBS solution) as the coating diluent, dilute the 9 TORCH coating materials to a concentration of 1.5mg / ml, and then streak coating on the PVDF membrane in sequence: quality Control detection line (C line) anti-human IgG, detection line Toxoplasma gondii (TOX) cleavage antigen and specific recombinant antigen, rubella viru...

Embodiment 2

[0081] 1. How to use the TORCH five-item IgG antibody (strip immunoblotting) detection kit:

[0082] 1) Take the TORCH five-item IgG antibody (strip immunoblotting) detection kit out of the refrigerator at 2-8°C and equilibrate to room temperature.

[0083] 2) Dilute the 20-fold concentrated lotion 1:20 into 1-fold lotion for later use.

[0084] 3) Take out the TORCH five-item IgG antibody (strip western blotting) detection reagent strip, put it into the reaction tank, add 1.5ml 1 times washing solution, and wash the membrane with a rocking table at room temperature (18-25°C) for 3 times, 5 minutes each time.

[0085] 4) Dilute the sample with the sample diluent at 1:50~1:100, add 1.5ml of the diluted sample, number it, and incubate on a rocking shaker for 30 minutes at room temperature (18-25°C), discard the sample diluent.

[0086] 5) Add 1.5ml of 1-fold washing solution, and wash the membrane 3 times on a rocking table at room temperature (18-25°C), 5 minutes each time. ...

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Abstract

The invention relates to a detection reagent of pathogenic microorganism, and particularly relates to an IgG antibody detection kit of TORCH five items and preparation of the kit. The detection kit is used for detecting IgG antibodies of TORCH five items, namely toxoplasma gondii (TOX), rubella virus (RV), cytomegalovirus (CMV) and herpes simplex 1-type and 2-type viruses (HSV-I/II) in a sample.

Description

Technical field: [0001] The invention relates to a detection reagent for pathogenic microorganisms, in particular to a TORCH five-item IgG antibody (strip immunoblotting) detection kit and a preparation method thereof. The kit is used to detect the five TORCH items in the sample, namely toxoplasma gondii (TOX), rubella virus (RV), cytomegalovirus (CMV) and herpes simplex virus type 1 and type 2 (HSV-I / II) IgG class of antibodies. Background technique: [0002] TORCH refers to the abbreviation of the English name of a group of pathogenic microorganisms that can cause congenital intrauterine infection and perinatal infection, thereby causing perinatal malformation, wherein T (Toxopasma) is Toxoplasma gondii, R (Rubella.Virus) is rubella virus, C (Cytomegalo.Virus) is cytomegalovirus, H (Herpes.Virus) is herpes simplex I / II type. TORCH is a group of microbial infections that share common features and can cause maternal and child infection. Pregnant women are prone to primary...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/569G01N33/531
Inventor 李艳召戈军曹建荣张誌
Owner BEIJING KINGHAWK PHARMA
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