7 results about "Types virus" patented technology

This invention discloses an attenuated chikungunya virus expressing a nanoluciferase gene, the nucleotide sequence of which is shown in SEQ ID NO:1. It is constructed by attenuating the virulence of the chikungunya virus LR2006-OPY1 strain by deleting 61 amino acids from positions 323 to 383 of the nsP3 protein and inserting a nanoluciferase gene after position 490 of the nsP3 protein. This virus model maintains similar growth characteristics to the wild-type virus but with significantly improved safety. Viral replication can be monitored in real time by detecting luciferase activity, making it suitable for drug screening at both cellular and animal levels. This provides an efficient and safe technical platform for the development of antiviral drugs against chikungunya virus.

The present invention relates to a composition for treating rhinovirus infectious diseases, comprising a GPR40 agonist as an active ingredient. Viruses causing cold symptoms include coronaviruses, picomaviruses, rhinoviruses, coxsackieviruses, adenoviruses, parainfluenza viruses, respiratory syncytial viruses, enteroviruses and the like, but since there is no universal antiviral agent capable of treating all types of viruses, application of the most appropriate therapeutic agent should vary depending on the virus causing the cold symptoms. The GPR40 agonist provided in the present invention exhibits a remarkable therapeutic effect on cold diseases caused by rhinovirus infection, and thus is expected to be actively used in the medical and healthcare fields.

This invention discloses PCR primers and probes for detecting ILT live vaccine virus and wild-type virus. The primers include a universal upstream primer: 5'-GCACAAAGTCCAAATCTTCT-3' and a universal downstream primer: 5'-TCAATCACAGACGGTACTTTC-3'. The probes include a vaccine virus-specific probe: 5'-FAM-CAAGTTGATGAATATGCTCAC-MGB-3' and a wild-type virus-specific probe: 5'-ROX-CAAGTTGATGAATGTGCTCA-MGB-3'. The 5' end of the vaccine virus probe is labeled with the FAM fluorescent group, and the 5' end of the wild-type virus probe is labeled with the ROX fluorescent group. The 3' ends of both probes are labeled with the MGB quencher group. Using these primers and probes, ILT live vaccine virus and wild-type virus can be detected and distinguished rapidly and accurately, and can be used for epidemic monitoring in farms.

This invention belongs to the field of viral genetic engineering technology and discloses a bovine herpesvirus type 1 (BHV-1) with three gene deletions, its construction method, and its applications. Using a Fosmid library, four myxovirus rescue combinations were screened. FosBHV-1-b-ΔTK-mCherry and FosBHV-1-d-ΔgEct / US9-eGFP were obtained through Red / ET and Gateway methods. These were then combined with FosBHV-1-a and FosBHV-1-c to construct an infectious cloning platform for recombinant BHV-1 with the TK / gEct / US9 three-gene deletions, rescuing recombinant viruses carrying tracer genes. This method has high recombination efficiency, and the cloning platform can efficiently construct BHV-1 gene-deleted strains. The immunogenicity of the deleted strains is comparable to that of wild-type viruses, with good safety, providing an efficient technical means for the development of BHV-1 attenuated vaccines and bovine disease live vector vaccines.

This invention belongs to the field of biodetection technology and relates to a method for specifically detecting wild-type virus residues in modified viral products and its application. This invention significantly suppresses non-specific extension into a mutant background by using LNA-modified primers; simultaneously, the SNP-embedded probe design differentiates at the signal level, amplifying the fluorescence signal differences between wild-type and mutant sequences. Finally, the ddPCR platform effectively eliminates PCR inhibitors and background noise from non-specific amplification. These three factors synergistically produce a significant enhancement effect. Taking VSV as an example, the embodiments demonstrate that this method can stably and repeatedly detect wild-type virus with a proportion as low as 1 / (10^7) against a mutant virus background of up to 10^10 copies, achieving a sensitivity at the part-in-ten-millions level. This represents an improvement of at least four orders of magnitude compared to traditional methods, far exceeding the reasonable expectations of those skilled in the art based on conventional technological improvements.

The application provides a kind of anti-RSV N protein monoclonal antibody and its preparation method and application, belong to monoclonal antibody technical field.The heavy chain variable region amino acid sequence of the monoclonal antibody described in the application is as shown in SEQ ID NO.2, the light chain variable region amino acid sequence is as shown in SEQ ID NO.4, is secreted by hybridoma cell strain 1A2, subtype is IgG1, Kappa type, has high affinity and strong specificity to RSV N protein.The antibody can specifically recognize RSV N protein and inactivated RSV A type virus, has high titer, good stability, and can be used for preparing respiratory syncytial virus detection reagent, especially suitable for double antibody sandwich ELISA detection kit, provides core material for early rapid detection of RSV infection, has important clinical application value.

The application provides a kind of real-time monitoring system and method based on wild animal host influenza virus, it is related to animal influenza virus monitoring technical field, comprising the following steps: step S1, dynamic monitoring point is laid out;Step S2, sample collection and pretreatment;Step S3, real-time detection and typing virus;Step S4, data real-time transmission and sharing;Step S5, virus sequence alignment and variation analysis;Step S6, dynamic risk assessment and early warning.The real-time monitoring system and method based on wild animal host influenza virus, through portable real-time fluorescent RT-PCR detection equipment realizes field real-time detection, combines double mode communication and cloud real-time analysis, eliminates the time lag of sample transportation and laboratory detection, realizes the real-time feedback of virus information.