203 results about "Types virus" patented technology

The invention relates to a human papilloma virus 18 L1 (HPV18L1) polynucleotide sequence and its expression vector, host cell and use and especially relates to an amino acid sequence of an HPV L1 capsid protein, a synthetic nucleotide sequence for coding the amino acid sequence, a recombinant expression vector containing the synthetic nucleotide sequence, and a hansenula polymorpha expression host strain containing the synthetic nucleotide sequence. The invention also relates to a use of an HPV18L1 protein composed of the amino acid sequence and derivatives of the amino acid sequence in preparation of vaccines. Through modification of a nucleotide sequence of a gene of an HPV18L1 wide-type virus, a recombinant HPV18L1 capsid protein can be highly expressed in a hansenula polymorpha expression system and thus hansenula polymorpha expression system-based industrial production of an HPV18L1 capsid protein is realized. Compared with the existing eukaryotic expression systems, the HPV18L1 polynucleotide sequence and its expression vector and host cell have advantages of higher yield and lower cost.

The invention provides a novel coronavirus antigen epitope and application thereof. A super computer is used for simulating S, E, M and N protein structures of the novel coronavirus, and novel coronavirus epitopes SEQ ID NO 1-38 are obtained through calculation. The epitopes have very good immunogenicity; an antibody generated by inducing a part of epitope polypeptide has the effect of neutralizing the novel coronavirus; the antigen epitope can be combined with antibodies in serum of novel coronavirus patients at home and abroad, has the potential of resisting various novel coronavirus strains, and also has the potential of identifying and applying different strains. The epitope provided by the invention can be used for (1) research and development of novel coronavirus vaccines and universal coronavirus vaccines such as MERS viruses and SARS viruses; and (2) preparing a novel coronavirus antibody, and further detecting and typing viruses and treating diseases caused by the viruses. Thenovel coronavirus antigen epitope has a wide application prospect in the aspects of prevention of coronavirus and propagation and outbreak thereof, and detection and diagnosis of virus strains.

The invention belongs to the medical technical field and discloses the application of high-purity in the preparation of antibacterial medicine, antiviral medicine and other medicines. The invention proves the antipyretic, anti-inflammation, immunity enhancement, antibacterial and anti-virus effects of high-purity forsythiaside through the following experiments: (1) the experiment that high-purity forsythiaside has antipyretic effect on fever rats; (2) the experiment that high-purity forsythiaside inhibits xylene-induced mice ear edema, xylene-reduced capillary permeability of mice; (3) the experiment that high-purity forsythiaside increases carbon clearance ability of mice and increases the lymphocyte transformation rate; (4) the experiment that high-purity forsythiaside has vitro anti-influenza A type virus effect and in-vivo anti-respiratory syncytial virus effect; (5) the experiment that high-purity forsythiaside has vitro inhibition effects on beta-hemolytic streptococcus, staphylococcus aureus, streptococcus viridans and staphylococcus epidermidis.

The invention relates to the field of molecular biology detection, in particular to detection of novel coronavirus, influenza A virus and influenza B virus. The invention provides a composition for detecting the viruses, and meanwhile, the invention further provides a kit containing the composition, application of the composition and a method for detecting and typing the viruses causing respiratory tract infection. The composition is combined with a fluorescent probe method, three viruses causing respiratory tract infection can be detected and typed at the same time in one tube, and the composition, the kit and the method have the advantages of being low in cost, high in flux, short in consumed time, easy and convenient to operate and capable of avoiding false positive caused by cross among samples and environmental pollution.

The invention relates to a coxsackie virus A16 type virus-like particle vaccine. The inventor fortuitously finds that proteins which have better spatial configurations and are suitably cut can be obtained by expressing P1 protein of CVA16 and 3CD protein of CVA16 by infecting insect cells with rhabdovirus. The proteins can be automatically assembled into a virus-like particle which has high immunogenicity.

The invention provides a coxsackie virus A16-type virus strain. The collection number of the coxsackie virus A16-type virus strain is CGMCC No.5372, wherein CGMCC refers to China General Microbiological Culture Collection Center. The virus is a 20-face stereoscopic symmetrical sphere under observation through an electron microscope, and the diameter of the virus is 23-30nm. VP1 conserved region sequence analysis and mass spectrum analysis are respectively performed on the virus strain, and a result shows a CA16 virus. The CA16 virus can be efficiently proliferated in Vero cells (African green monkey kidney cells), and the virus titer can reach 6.61g CCID50/ml. Moreover, the virus strain has no external pollution, better immunogenicity and a good effect.

The invention relates to a Coxsackie A16 type virus mutant strain capable of effectively infecting mice. In the invention, a mouse-adaptive mutant virus strain is unexpectedly obtained through continuous passage of CA16 virus by using the mouse cells and mouse; the mouse-adaptive strain has very strong mouse infection ability and can be used for preparing a mouse model of Coxsackie virus infection so as to study the pathogenic mechanism of the virus, evaluate the antiviral drugs, etc.

The invention discloses a suspension culture production method for porcine circovirus 2-type cells; the method provided by the invention comprises the production technology of preparing porcine circovirus 2-type inactivated vaccines by suspension culture for poisoned cells through porcine circovirus 2-type virus biological reactor micro carriers. The method provided by the invention can greatly reduce production cost and increase output-input ratio by 5-10 times; and the method shortens production period by 2-3 days and occupies small places, wherein occupied area is only 1/5 of the occupied area in the traditional roller bottle process under the same yield; scale of production is enlarged fast and easily; pollution is little and processes are performed easily under the totally-enclosed tank production condition; degree of automation is high; employee is less; quality stability is realized easily; and the method can significantly reduce production cost and increase yield and quality of vaccines.

The invention belongs to the technical field of biological medicine and biological detection and particularly relates to antigen epitope minimum motif peptide of a human papilloma virus (HPV) 58 type E6 protein and an application thereof. The invention provides four epitope peptides (comprising an 8-peptide sequence which can be expressed in a fusion way with carrier proteins such as truncated GST188 and the like and contain epitope minimum motif) which can be used as antigens for specifically detecting HPV 58 type virus infection in a single way or a combination way and contains the minimum motif and a marker minimum motif peptide amino acid sequence used for specifically detecting low risk type HPV virus infection and medium-high risk type HPV virus infection in a discrimination way. The amino acid sequences of the minimum motif peptides and short peptides are shown as SEQ ID No.1-SEQ ID No.9.

The invention discloses a bactrian camel VHH (variable domain of the heavy chain of HACbs) heavy-chain antibody for resisting foot-and-mouth disease AsiaI type viruses, a preparation method and use thereof, belonging to the field of biotechnology. In the method, recombinant AsiaI type virus-like particle antigens are used for immunizing bactrian camels; an AsiaI VHH immune antibody library is established with phage display technology; high-specificity single-domain antibodies having good affinity with foot-and-mouth disease AsiaI type virus antigens are screened; the antibody provided by the invention has amino acid sequence shown by SEQ ID NO.1 or amino acid sequence which has sequence homology more than 98% with the sequence shown by SEQ ID NO.1. The AsiaI VHH heavy-chain antibody provided by the invention has great importance for researching AsiaI type virus infection mechanisms and producing high-sensitive and specific antigen fast detection or diagnosis reagents.

The invention relates to a hybridoma cell strain and a monoclonal antibody secreted by the hybridoma cell strain for resisting foot-and-mouth disease O-type viruses and an application. The foot-and-mouth O-type (O/Mya98/BY/2010) virus capable of inducing the immune reaction of the body is used as immunogen to obtain the hybridoma cell strain 1F2 capable of sustainably and stably secreting the monoclonal antibodies for resisting the foot-and-mouth disease O-type (O/Mya98/BY/2010) virus, and the cell strain secretes the monoclonal antibody 1F2anti. The monoclonal antibody can specifically identify the foot-and-mouth disease O-type (O/Mya98/BY/2010) virus, can be used for detecting the foot-and-mouth disease O-type (O/Mya98/BY/2010) virus, has advantages of high specificity and high sensitivity and can be applied to the detection of the foot-and-mouth disease O-type (O/Mya98/BY/2010) virus, vaccine production and epidemiology research.

An embodiment of the invention discloses a pig circovirus III-type virus-like particle. A preparation method of the virus-like particle includes the steps: amplifying pig circovirus III-type Cap protein genes; constructing recombinant shuttle-plasmid pFB-Cap by the aid of the genes; constructing rB-Cap recombinant rod granules by the aid of the recombinant shuttle-plasmid pFB-Cap; transfecting therB-Cap recombinant rod granules into SF9 cells to obtain recombinant baculovirus rBV-PCV3 Cap expressing pig circovirus III-type Cap genes; enabling the recombinant baculovirus rBV-PCV3 Cap to infectHigh Five cells, and purifying the recombinant baculovirus rBV-PCV3 Cap infected by the High Five cells to obtain the pig circovirus III-type virus-like particl PCV3 VLP. A nucleotide sequence of theCap protein gene is as shown in SEQ ID NO.1. According to the particle, based on a baculovirus-insect cell expression system, preparation is implemented by the aid of High Five cell expression of serum-free suspension culture, and the PCV3 virus-like particle is acquired by combining sucrose cushion ultracentrifugation and sucrose density gradient centrifugation purification. The virus-like particle is good in immunogenicity and high in safety and has good development and application prospects.

The invention discloses an ELISA detection kit for a foot-and-mouth disease O-type virus sIgA antibody and an application thereof. The kit comprises a foot-and-mouth disease O-type virus broad spectrum multi-epitope recombinant antigen coated enzyme labeled reaction plate, a 100*concentrated enzyme labeled antibody, an enzyme labeled antibody diluent, a sample diluent, a concentrated cleaning solution, a developing solution, a stop solution, a positive control sample and a negative control sample. The foot-and-mouth disease O-type virus broad spectrum multi-epitope recombinant antigen is composed of main neutral epitopes of Cathay, PanAsia and Burmese 98 three pedigrees of foot-and-mouth disease O-type virus typical strains, so that the sensitivity and specificity of kit detection can be promoted and the kit is suitable for detection of different foot-and-mouth disease O-type virus infections. The kit provided by the invention is suitable for detection of sIgA antibodies in easily infected animal mucosal secretion of pig, cattle and sheep and has a great significance in preventing and controlling the spreading and infection of foot-and-mouth disease O-type virus.

The invention discloses a virus detection method. The virus detection method comprises obtaining a program to be detected and decompiling the program to be detected; extracting instruction sequence features of the post-decompiling program to be detected; detecting viruses of the program to be detected according to the extracted instruction sequence features. The virus detection method can effectively and quickly detect inflection type viruses of specific types, is low in false positive rate, and, by means of detection of instruction sequence features, is wide in detection range compared with a traditional feature code method, thereby improving detection efficiency. The invention further discloses a virus detection device and a terminal.

The invention belongs to the field of biotechnologies, and particularly relates to a multi-PCR-ELISA detection kit for human seasonal influenza viruses and an application method thereof. The detection kit disclosed by the invention is composed of a RT-PCR reaction system, an ELISA detection system, four target-gene (an influenza-A-virus M gene, a H1 subtype virus HA gene, a H3 subtype virus HA gene, and a B type virus NS gene) positive plasmids (Pa-m, Ph1-ha, Ph3-ha and Pb-ns), a negative quality control specimen and four target-gene specific primer probes. Specific amplification is performed by using specific primers labeled by using four sets of biotins through RT-PCR, an amplified product after being denatured is hybridized with a specific probe labeled by using digoxin, a hybridized product is enveloped with a streptavidin-enveloped 96-hole micro-plate, and an anti-digoxin antibody labeled by using horse radish peroxidase is added for carrying out detection through an ELISA method, so that a rapid, sensitive and specific typing detection kit for seasonal influenza viruses such as H1 and H3 subtypes and hepatitis B viruses is established. The invention relates to the application of four sets of specific primer probes in the clinical differential diagnosis of influenza virus infection and the typing authentication of influenza virus isolates.

The invention relates to preparation of enterovirus 71 type virus-like particle by using a baculovirus recombination protein expression system and application of the virion. By modifying baculovirus vector design, hydrolysis protease 3CD expression is reduced and structural protein predecessor protein P1 is enhanced to increase virus-like particle (VLP) expression. The enterovirus virus-like particle can initiate high antibody titer reaction and neutralization titer reaction of mice, thereby indicating that the VLP can be used as vaccine of the enterovirus 71 type.

The invention discloses a hybridoma cell strain 6D6 of a specificity monoclonal antibody capable of continuously and stably secreting foot-and-mouth disease resistant O-type (O/GX/09-7) viruses, and the specificity monoclonal antibody secreted by the hybridoma cell strain. The 6D6anti can recognize the foot-and-mouth disease O-type (O/GX/09-7) viruses in a specificity mode, the 6D6anti is used as a coating and an enzyme labeled antibody in ELISA detection and can be used for detecting the foot-and-mouth disease O-type (O/GX/09-7) viruses, and the advantages of high specificity and high sensitivity are achieved; the hybridoma cell strain, the antibody and the application can play an important role in detection of the foot-and-mouth disease O-type (O/GX/09-7) viruses, vaccine production and epidemiologic study.

An interior-applied Chinese medicine for treating the Yin-blood deficiency type viral myocarditis is prepared from 13 Chinese-medicinal materials including red sage root, pilose asiabell root, flavescent sophora root, coptis root, etc.

The invention provides a purification method of porcine circovirus II type virus-like particles. The method is a new purification method developed through experiment measures centring on the self-characteristics of the PCV2 virus-like particles and the characteristics of an expression system of the PCV2 virus-like particles. The method comprises the steps that firstly, cell lysis is achieved through comprehensive utilization of freezing-thawing and ultrasonic treatment measures; secondly, a great amount of cell debris and macromolecule impurities are removed by means of a filter plate module, and sterile supernatant liquid is obtained; thirdly, the supernatant liquid is concentrated by means of a 100kD hollow fiber membrane system, and a concentrated virus antigen solution is obtained; fourthly, chromatographic purification is conducted by means of a Sepharose 4FF molecular sieve chromatographic purification system in optimized operation conditions. The method is designed closely aiming at the characteristics of the PCV2 virus-like particles, and particularly suitable for the purification of recombinant baculovirus PCV2 virus-like particles. By means of the method, the impurities can be more efficiently removed and separated to obtain target proteins, and target antigens can be effectively recycled.

The invention provides a replicating type recombinant adenovirus HAdV-5 carrier system. The system comprises a framework plasmid pKAd5, an intermediate plasmid pMDXE3YA and a shuttle plasmid pKNXE3M;a target gene coding region is cloned to multiple cloning sites of the shuttle plasmid, EcoRI digests the shuttle plasmid carrying a target gene, and an obtained fragment is used for replacing the corresponding part of the intermediate plasmid; then NdeI is used for digesting the newly-constructed intermediate plasmid, and an obtained fragment is used for replacing the corresponding part of the framework plasmid so as to obtain a recombinant adenovirus plasmid; and the PacI linearized recombinant adenovirus plasmid is used to transfect a 293 cell so as to prepare a recombinant HAdV-5 adenovirus with target gene expression controlled by an HAdV-5 major later promoter (MLP). according to the system, the recombinant adenovirus can be replicated and proliferated in various human cells, is a replicating type virus and is expected to have a wide application prospect in the research and development of animal oral carrier vaccines.

The embodiment of the invention provides a virus detection method and device, and the method comprises the steps: carrying out the search from a preset virus analysis website according to the annotation of a preset virus classification keyword of a ransovirus, and obtaining the suspected virus information of the ransovirus; acquiring characteristic parameters in the suspected virus information; and detecting whether the suspected virus information is ransovirus type virus information according to the characteristic parameters. According to the embodiment of the invention, the timeliness of virus detection is improved.

The present invention provides a swine fever virus chimeric type virus-like particle, a preparation method thereof, an application thereof and a vaccine, and belongs to the technical fields of bioengineering and virus vaccines. The swine fever virus chimeric type virus-like particle selects a Gag precursor protein of retroviruses as a matrix protein and swine fever virus envelope proteins E0 and E2 as surface membrane proteins to self-assemble into the ideal and functional virus-like particle, weaknesses of a subunit protein vaccine are overcome, and immunogenicity, safety and stability of thevaccine are effectively improved. The swine fever virus chimeric type virus-like particle can be prepared in large quantities by constructing expression vectors to transfect cells. The swine fever virus chimeric type virus-like particle can be applied to prepare the vaccine for preventing swine fever epidemic diseases, a swine fever virus antibody detection preparation or a swine fever virus epidemic disease monitoring preparation, and has a relatively good practical application value.