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Nucleic acid detection kit for quickly detecting measles virus/rubella virus

A technology for detecting kits and measles virus, applied in the direction of microorganism-based methods, microorganism measurement/inspection, microorganisms, etc., can solve the problems of poor specificity and low sensitivity, and achieve high sensitivity, good specificity, and rapid detection results objective effect

Active Publication Date: 2013-11-27
JIANGSU BIOPERFECTUS TECH CO LTD
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The technical problem mainly solved by the present invention is to overcome defects such as poor specificity and low sensitivity of existing measles virus / rubella virus detection kits, and provide a nucleic acid detection kit for rapid detection of measles virus / rubella virus. With the advantages of high throughput, easy operation, strong repeatability, rapid and objective detection results, etc., it has a good application prospect for the detection of measles virus / rubella virus

Method used

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  • Nucleic acid detection kit for quickly detecting measles virus/rubella virus
  • Nucleic acid detection kit for quickly detecting measles virus/rubella virus
  • Nucleic acid detection kit for quickly detecting measles virus/rubella virus

Examples

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Embodiment 1

[0035] Example 1 A nucleic acid detection kit for rapid detection of measles virus / rubella virus

[0036] The above kit includes RT-PCR reaction solution, enzyme mixture, measles virus / rubella virus reaction solution, positive control and negative control;

[0037] Wherein, the RNase inhibitor is DEPC water (wherein, 1ml DEPC is added in 1L water); RT-PCR reaction solution includes 10× buffer, 25mM MgCl 2 and 10mM dNTPs;

[0038] Measles / Rubella Reaction Solution includes the following components:

[0039]Component (1): consists of a pair of primers for detecting measles virus and a probe for detecting measles virus; wherein, the base sequences of the two primers are shown in SEQ ID No.1 and SEQ ID No.2 respectively; The base sequence of the needle is shown in SEQ ID No.3, the 5' end of the probe is marked with a fluorescent reporter group, and the 3' end is marked with a fluorescent quencher group; the primer SEQ ID No.1 for detecting measles virus is 500nM, SEQ ID No.2 is...

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Abstract

The invention discloses a nucleic acid detection kit for quickly detecting a measles virus / a rubella virus. The kit comprises an RT-PCR (Reverse Transcription-Polymerase Chain Reaction) reaction liquid, an enzyme mixed liquid, a measles virus / rubella virus reaction liquid, positive control and negative control. The kit disclosed by the invention overcomes the deficiencies that the prior art, almost measles virus / rubella virus detection kits are poor in specificity, relatively low in sensitivity and the like, has the advantages of high throughput, strong repeatability, quick and objective detection result and the like, is simple and convenient to operate and has huge application prospect in the fields such as clinical diagnosis and disease surveillance.

Description

technical field [0001] The invention relates to a nucleic acid detection kit, in particular to a nucleic acid detection kit for rapid detection of measles virus / rubella virus, belonging to the field of biotechnology. Background technique [0002] Measles is an acute respiratory infectious disease caused by measles virus infection. It is mainly transmitted through droplets. The clinical symptoms include fever, cough, runny nose, conjunctival hyperemia, and blushing gray spots on the oral mucosa. The prognosis of simple measles is good, and the fatality rate of severe patients is high. The infected person is the only source of infection. From 2 days before the onset (the end of the incubation period) to 5 days after the onset of the rash, the conjunctival secretions, nasal, oropharyngeal, and tracheal secretions all contain viruses and are infectious. Measles virus is the pathogen of measles, which belongs to the genus Morbillivirus of the family Paramyxoviridae in classific...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/70C12Q1/68C12R1/93
Inventor 李荣宇王国强刘中华杨静张旭
Owner JIANGSU BIOPERFECTUS TECH CO LTD
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