39 results about "Disease surveillance" patented technology

The disclosure relates to jointly transmitting and processing analog-digital information using Inaudible Synchronous Online User-optimized Processing (iSOUP), wherein digital and analog information can be transmitted via one connection. iSOUP may be implemented in real-time MP3 anti-piracy, customized MP3 songs, Internet customized medical devices, Internet customized consumer electronics, Internet controlled psychoacoustic and physiological tests, tinnitus treatment devices, anti-piracy MP3 players, assistive listening MP3 players, healthcare sensor networks, Bluetooth sensors, audio-visual hearing aids (HAs) and cochlear implants (CIs), audio-touch HAs and CIs, Bluetooth HAs and CIs, automated fitting of HAs and CIs. The invention has applicability in copyright protection, data hiding, hearing aid remote controls, children learning devices, musical special effects generators, multiuser multimedia devices, Internet treatment and disease surveillance, wireless medical devices, wireless telemetry, multimedia rehabilitation devices, hearing aids and other implants.

The invention provides RPA primers, probes, kits and detection methods for detecting Staphylococcus aureus. The RPA primers for detecting Staphylococcus aureus of the present invention include upstream primer Seq ID No.1 and downstream primer Seq ID No.2 for clfA gene. Compared with the prior art, the kit and detection method of the present invention can quickly detect whether there is Staphylococcus aureus nucleic acid in a sample. The operation is simple, no special and expensive instruments are required, and it has important application value in the fields of disease monitoring, clinical diagnosis and food safety monitoring.

The invention discloses a greenhouse vegetable disease surveillance video key frame extracting method and extracting system. According to the extracting method and the extracting system, the visual significance is combined with an online clustering algorithm. Firstly, an X2 histogram method is utilized to measure interframe difference and remove the effects of video frame images with the similar characteristics on the algorithm calculation amount; then the video frame images are transferred to an HSV color space, a visual significance diagram is calculated through characteristics of greenhouse vegetable surveillance video, an H channel and an S channel to extract the significance areas in the video frame images, and scab information, possible to be lost, in the significance areas is repaired through a morphological method; finally, key frames can be extracted through the online clustering algorithm and a pixel frame average algorithm. The extracting method can effectively obtain disease information in greenhouse vegetable surveillance video to establish solid foundation for accurate identification on greenhouse vegetable diseases. If combined with image processing techniques and mode identifying techniques, the extracting method and the extracting system can make great contributions to greenhouse vegetable disease identification.

The invention relates to an oligonucleotide sequence combination for specifically detecting existence of streptococcus pneumoniae and haemophilus influenzae nucleic acids in samples by adopting fluorescent PCR (polymerase chain reaction) technology and a kit comprising the combination. The kit can sensitively detect and identify the streptococcus pneumoniae and haemophilus influenzae nucleic acids in the samples, has limits of detection of 20 copies per reaction system and has important application values in the fields such as disease surveillance and clinical diagnosis.

A digital disease control information integration and intelligent public health management platform comprises a management platform server, a database server, an OA office system, a disease surveillance system, an inspection report system, a medical information system, a child preventive vaccination management system, an internal network, an external network, a child preventive vaccination outpatient department, a disease surveillance terminal, a health hazard factor monitoring terminal, a serious disease special reporting system, a public health emergency commanding system, an expert system, a medical information workstation, a monitoring terminal, a testing laboratory, a subordinate unit system, a superior unit system and an information searching and publishing system. All disease data collection and the whole data monitoring of diseases are achieved, and data samples can cover all people and the whole periods of the diseases. Dynamic quality control management is achieved, and therefore management quality and the accuracy of the data are guaranteed, quality control cost and administrative management cost are lowered, and disease prevention and management efficiency is improved.

The invention discloses an RT-LAMP (Reverse Transcription Loop-Mediated Isothermal Amplification) kit for detecting avian influenza virus subtype H7N9. The kit comprises six pairs of primers, the nucleotide sequences are shown in SEQ ID No.1-12 respectively. The kit can rapidly, specifically and sensitively detect the avian influenza virus subtype H7N9, a turbidity meter is utilized for judging the reaction in real time, or SYBR Green I is injected after the reaction is finished, and a detection result is judged by color reaction; the lowest detection limit of a primer for detecting HA gene is 48fg/mul of H7N9 RNA; and the lowest detection limit of a primer for detecting NA gene is 4.8pg/mul of H7N9 RNA. The kit is convenient and simple to use, low in cost and good in specificity, is very suitable for disease surveillance, on-site emergency and clinical specimen detection, and is suitable for large-range popularization and application, and the reaction result is easy to observe.

The invention provides an RPA (recombinase polymerase amplification) primer, probe, kit and method for detecting vibrio parahaemolyticus. The RPA (recombinase polymerase amplification) primer for detecting the vibrio parahaemolyticus comprises a forward primer with the sequence as shown in Seq ID No.1 in the specification and a reverse primer with the sequence as shown in Seq ID No.2 in the specification aiming at an irgb gene. Compared with the prior art, the kit and the method have the advantages that whether nucleic acid of the vibrio parahaemolyticus exists in a sample or not can be rapidly detected; and the operation is simple, special and expensive instruments are not needed, and important application values in the fields of disease surveillance, clinical diagnosis, food safety inspection and the like are achieved.

The invention relates to the technical field of biology, and discloses a LAMP visual rapid detection kit of silkworm pathogenic micro spore worms and a detection method of the LAMP visual rapid detection kit of the silkworm pathogenic micro spore worms. The kit comprises four LAMP primers, deoxyribonucleic acid (DNA) lysate liquid, LAMP reaction liquid, positive reference products, negative reference products and color development liquid to form a detection reaction system. Under the constant temperature of 60 DEG C to 65 DEG C, formwork DNA is rapidly amplified, developed dye is added to the formwork DNA, and identification results are observed through naked eyes under natural light. If the color of reaction products changes into green, the fact that samples to be tested contain the silkworm pathogenic micro spore worms is confirmed, and if the color of reaction products changes into orange, the fact that the samples to be tested do not contain the silkworm pathogenic micro spore worms is confirmed. The kit can detect current various silkworm pathogenic micro spore worms fast and flexibly, is easy to operate, and low in cost, and needs no special or complex instruments, and reaction results are easy to judge, and specificity is strong. The kit can meet the urgent needs of disease surveillance, on-site emergency situation and the detection of production samples, and can be widely popularized and applied easily.

The invention discloses a nucleic acid detection kit for rapidly detecting respiratory syncytial viruses A and B and an application of the nucleic acid detection kit. The kit comprises the following steps: a nucleic acid amplification reaction liquid, enzyme mixed liquor, a reaction liquid of respiratory syncytial virus A/B, positive control and blank control. According to the nucleic acid detection kit disclosed by the invention, the defects that the nucleic acid detection kit for the respiratory syncytial viruses A and B in the prior art is poor in specificity, relatively low in sensitivity and the like are overcome. The nucleic acid detection kit for the respiratory syncytial viruses A and B disclosed by the invention has the advantages of being high in flux, simple and convenient to operate, high in repeatability, fast and objective in detection result, and has a great application prospect in the aspects such as clinical diagnosis of the respiratory syncytial viruses A and B and disease surveillance.

The invention discloses a microscale fibre-optical probe as well as a method and a device for detecting content of glucose. A micro-nanofiber of which the surface is modified by glucose oxidase (GOD) is taken as a probe. A measurement principle is as follows: when light waves are transmitted in the micro-nanofiber probe, evanescent waves exist outside the micro-nanofiber, is transmitted along the axial direction of the micro-nanofiber, and interacts with the environment outside the micro-nanofiber. Glucose is converted into gluconic acid under catalytic action of glucose oxidase modifying the surface of the micro-nanofiber, the micro-nanofiber causes change of the refractive index of the environment, so that the evanescent waves have phase modulation during transmission in the area and therefore change of the equivalent optical path of the micro-nanofiber is caused. By measuring the change information of optical path caused by change of the concentration of glucose, the content of the glucose is measured. The microscale fibre-optical probe as well as the method and the device have the advantages that the sensitivity and response speed are very high, the measurement range is wide, and the reliability is high, and application requirements in the fields including food safety, disease surveillance, clinical care and the like can be met.

The invention relates to a primer probe composition and a kit for specificity detection and identification of neisseria meningitidis, which adopts a real-time fluorescent polymerase chain reaction (PCR) method to specifically detect a nucleotide sequence composition existing in neisseria meningitidis nucleic acid in a sample and comprises the kit of the composition. By means of the sequence composition and the kit, whether the neisseria meningitidis nucleic acid exists or not in the sample can be rapidly detected, simultaneously whether the neisseria meningitidis nucleic acid belongs to the fashionable A group or C group in our country or not is identified. The limit of detection of the kit on the neisseria meningitidis (commonly used) is 200 copies per reaction system. The limit of detection on the neisseria meningitidis in the A group is 200 copies per reaction system, and the limit of detection on the neisseria meningitidis in the C group is 200 copies per reaction system. The whole reaction can be finished in 2 hours, and the primer probe composition and the kit have important application value in the fields of disease surveillance, clinical diagnosis and the like.

The invention relates to an oligonucleotide sequence combination for specific detection of bordetella pertussis and bordetella parapertussis nucleic acids existing in a sample with the adoption of fluorescent PCR (polymerase chain reaction), and a kit containing the combination. The kit can sensitively detect the bordetella pertussis nucleic acid and the bordetella parapertussis nucleic acid existing in the sample with a detection lower limit of 20 copies in each reaction system, and has important application value in the fields such as disease surveillance and clinical diagnosis.

The invention discloses a nucleic acid detection kit for quickly detecting a measles virus/a rubella virus. The kit comprises an RT-PCR (Reverse Transcription-Polymerase Chain Reaction) reaction liquid, an enzyme mixed liquid, a measles virus/rubella virus reaction liquid, positive control and negative control. The kit disclosed by the invention overcomes the deficiencies that the prior art, almost measles virus/rubella virus detection kits are poor in specificity, relatively low in sensitivity and the like, has the advantages of high throughput, strong repeatability, quick and objective detection result and the like, is simple and convenient to operate and has huge application prospect in the fields such as clinical diagnosis and disease surveillance.

The invention relates to a wireless community-based air disease surveillance and prevention health care system which is used for monitoring air quality. The wireless community-based air disease surveillance and prevention health care system comprises seven modules including a gas sensor module (1), a power module (2), a data acquisition and conversion module (3), a local transmission wireless module (4), a control and early-warning forecast module (5), a health care information publish module (6) and a user terminal module (7). The system has the advantages that the air quality in the living area can be forecasted and compared with the information of a professional database, so that disease prevention and rehabilitation instructions in the daily life can be provided for the people in the living area, the health degree of the people is effectively improved, and a favorable condition is provided for city disease control and prevention.

The invention discloses a split type wireless multipath video acquisition display system. The system comprises a camera acquisition module, a data processing and wireless video transmitting module; the camera acquisition module is formed by a flexible circuit board, one end of the flexible circuit board is provided with a camera, and the other end is provided with a connecting base in detachable connection with the data processing and wireless video transmitting module; the data processing and wireless video transmitting module is provided with a connecting piece in match connection with the connecting base of the camera acquisition module; the system further comprises a wireless transmitting chip; the camera and an optical source on the flexible circuit board are used for acquiring image signals in different directions, the image signals are analyzed, compressed and stored through the data processor and then transmitted through the data processing and wireless video transmitting module after the connecting piece and the connecting base are connected, and finally, the image signal is displayed through an external wireless receiving display end. Higher definition and faster video acquisition transmission efficiency can be realized through the system disclosed by the invention, the cost is lowered, and the system is capable of fully monitoring the disease surveillance stage.

The invention discloses a portable disease surveillance recorder which comprises a shell and a central processing unit arranged in the shell and further comprises a display screen, a light sensitization module, a camera module, an infrared light supplementing module, a key module, a 3G communication module, a pickup module, a storage module, a power supply module, a GPS (Global Positioning System) module and a USB (Universal Serial Bus) module, wherein the central processing unit is respectively connected with the display screen, the light sensitization module, the camera module, the infrared light supplementing module, the key module, the 3G communication module, the pickup module, the storage module, the power supply module, the GPS module and the USB module; and the light sensitization module comprises a light sensitizer and an infrared lens, the input end of the light sensitizer is connected with the infrared lens and the output end of the light sensitizer is connected with the central processing unit. The portable disease surveillance recorder has multiple functions of photograph, camera shooting, recording pens and the like and integrates a GPS technology and a 3G technology, thereby greatly improving the accuracy and the timeliness of disease information collection works and rapid emergency response capability.

The invention relates to a non-enzymatic catalytic sensor for glucose and a preparation method thereof. Meso-Tetrakis (4-(N-methylpyridyl)) porphyrin iron (III) molecule (abbreviated as FeTMPyP) self-assembles calixarene-modified gold nanoparticles into three-dimensional gold nanostructures, the system of which will be constructed onto a gold electrode modified with the same calixarene so that a glucose sensor composed of three-dimensional gold nano-particles is produced, the hydrogen peroxide and glucose is catalyzed by a double catalytic system under electrochemical conditions to achieve thesame measuring effect as a glucose sensor. The invention utilizes the self-assembly of the three-dimensional structure of the gold nanometer by the porphyrin, and greatly expands the application of the calixarene functionalized gold nanoparticle, so as to facilitate the greater application for the functionalized gold nanoparticles in the future production, human lives and researches. The invention also provides a more convenient method for the detection of glucose in disease surveillance and industrial production.

The invention belongs to the technical field of molecular diagnosis and verification, and relates to a LAMP visual rapid detection kit of silkworm densoviruses and a detection method of the LAMP visual rapid detection kit of the silkworm densoviruses. The kit comprises four LAMP primers, deoxyribonucleic acid (DNA) extraction buffer solutions, LAMP reaction liquid, positive reference products, negative reference products and color development liquid to form a detection reaction system. A boiling precipitation method is adopted to fast extract the formwork DNA of samples to be detected, the formwork DNA is rapidly amplified under the constant temperature of 60 DEG C to 65 DEG C, developed dye is added to the formwork DNA, and identification results are observed through naked eyes under natural light. If the color of reaction products changes into green, the fact that the samples to be tested contain the silkworm densoviruses is confirmed, and if the color of reaction products changes into orange, the fact that the samples to be tested do not contain the silkworm densoviruses is confirmed. The kit has the advantages of being high in sensibility, strong in specificity, swift in reaction, easy to operate, and low in cost, and needs no special instruments or equipment, and reaction results are easy to observe and judge. The defects that the detection of the silkworm densoviruses is long in time, huge in workload, complex in operation and the like are overcome. The kit can meet the urgent needs of disease surveillance, on-site emergency situation and the detection of production samples.

The invention discloses a multiplex fluorescence quantitative PCR detection kit for avian influnza virus N subtype genetic typing and the application thereof. The kit comprises a nucleic acid amplified reaction liquid, an enzyme mixed liquid, an avian influnza virus N subtype reaction liquid A, an avian influnza virus N subtype reaction liquid B, an avian influnza virus N subtype reaction liquid C, a positive control A, a positive control B, a positive control C and a blank control. The kit overcomes the deficiencies in the prior art that most of avian influnza virus N subtype nucleic acid typing detection kits are poor in specificity and low in sensitivity, has the advantages of high throughput, high convenience in operation, high repeatability and rapid and objective detection results, and has a great application prospect in terms of clinical diagnosis and disease surveillance of avian influnza virus N subtype.

The invention provides an infectious pancreatic necrosis recombinant VP2 protein antigen and a purification method. In the invention, viral nucleic acid (RNA) is extracted from infectious pancreatic necrosis virus cell culture liquid, a downstream primer P2 of an IPNV VP2 gene is used for synthesizing a virus genome cDNA, an upstream primer and the downstream primer of the IPNV VP2 gene are utilized to amplify the VP2 gene, the IPNV VP2 gene obtained by amplification is cloned into a prokaryotic expression carrier plasmid pET30b, and expression is carried out in a colon bacillus BL21 strain to obtain the infectious pancreatic necrosis recombinant VP2 protein antigen. The antigen can overcome the potential hazards of toxin expulsion and toxin dispersion existing in an antigen in the traditional diagnosis method of infectious pancreatic necrosis, and can be used as a diagnostic antigen for disease surveillance.

This disclosure describes portable bio-nano-chip assays, methods and compositions for diagnosing and assessing pathogen-mediated diseases or infections at point-of-care using biological samples. The assays, methods and compositions provide in a more convenient, less expensive, and less time-consuming sampling and analysis.