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Nucleic acid detection kit for rapidly detecting respiratory syncytial viruses A and B and application of nucleic acid detection kit

A detection kit and syncytial virus technology, applied in the biological field, can solve the problems of poor specificity and low sensitivity, achieve high sensitivity, good specificity, and reduce the chance of pollution

Active Publication Date: 2015-05-06
JIANGSU BIOPERFECTUS TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] The technical problem to be solved by the present invention is to overcome the defects of poor specificity and low sensitivity of the existing RSV type A and B type detection kits, and provide a nucleic acid for rapid detection of RSV type A and B types. Detection kit, the kit has the advantages of high throughput, easy operation, strong repeatability, rapid and objective detection results, etc., and has a good application prospect for the detection of respiratory syncytial virus types A and B

Method used

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  • Nucleic acid detection kit for rapidly detecting respiratory syncytial viruses A and B and application of nucleic acid detection kit
  • Nucleic acid detection kit for rapidly detecting respiratory syncytial viruses A and B and application of nucleic acid detection kit
  • Nucleic acid detection kit for rapidly detecting respiratory syncytial viruses A and B and application of nucleic acid detection kit

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Embodiment 1

[0039] Example 1 Preparation of a nucleic acid detection kit for rapid detection of respiratory syncytial virus type A and type B

[0040] The kit includes a nucleic acid amplification reaction solution, an enzyme mixture, a respiratory syncytial virus type A / B reaction solution, a positive control and a blank control;

[0041]Wherein, the nucleic acid amplification reaction liquid includes Tris, potassium chloride, magnesium chloride, and nucleotide mixture;

[0042] The Respiratory Syncytial Virus Type A / B Reaction Solution contains the following components:

[0043] Component (1): consists of three primers for detecting respiratory syncytial virus type A / B; wherein, the base sequences of the three primers are respectively shown in SEQ ID No.1, SEQ ID No.2 and SEQ ID No.3 Show; The primer concentration that detects respiratory syncytial virus type A / B type is: SEQ ID No.1 is 600nM (nmol / L), SEQ ID No.2 is 600nM, and SEQ ID No.3 is 600nM;

[0044] Component (2): consists of...

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Abstract

The invention discloses a nucleic acid detection kit for rapidly detecting respiratory syncytial viruses A and B and an application of the nucleic acid detection kit. The kit comprises the following steps: a nucleic acid amplification reaction liquid, enzyme mixed liquor, a reaction liquid of respiratory syncytial virus A / B, positive control and blank control. According to the nucleic acid detection kit disclosed by the invention, the defects that the nucleic acid detection kit for the respiratory syncytial viruses A and B in the prior art is poor in specificity, relatively low in sensitivity and the like are overcome. The nucleic acid detection kit for the respiratory syncytial viruses A and B disclosed by the invention has the advantages of being high in flux, simple and convenient to operate, high in repeatability, fast and objective in detection result, and has a great application prospect in the aspects such as clinical diagnosis of the respiratory syncytial viruses A and B and disease surveillance.

Description

technical field [0001] The invention relates to a nucleic acid detection kit and an application thereof, in particular to a nucleic acid detection kit for rapidly detecting types A and B of respiratory syncytial virus and an application thereof, belonging to the field of biotechnology. Background technique [0002] Respiratory syncytial virus (respiratory syncytial viruses, referred to as RSV) is a kind of RNA virus belonging to Paramyxoviridae. It is one of the most common causes of lower respiratory tract viral infections in infants and young children worldwide, especially for infants aged 2-6 months who often develop severe bronchiolitis and pneumonia after being infected with RSV. In addition, it is also an important pathogen that causes the exacerbation of chronic bronchitis in the elderly and lower respiratory tract infection in children with immune impairment. [0003] RSV is currently divided into A subtype and B subtype, and the repeated infection of RSV may be rel...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/70C12Q1/68C12R1/93
CPCC12Q1/6851C12Q1/70C12Q1/701C12Q2600/16C12Q2531/113C12Q2537/143C12Q2561/101
Inventor 李秀林杨静高铭鞠刘中华王国强张旭
Owner JIANGSU BIOPERFECTUS TECH CO LTD
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