Infectious pancreatic necrosis recombinant VP2 protein antigen and purification method

Abstract

The invention provides an infectious pancreatic necrosis recombinant VP2 protein antigen and a purification method. In the invention, viral nucleic acid (RNA) is extracted from infectious pancreatic necrosis virus cell culture liquid, a downstream primer P2 of an IPNV VP2 gene is used for synthesizing a virus genome cDNA, an upstream primer and the downstream primer of the IPNV VP2 gene are utilized to amplify the VP2 gene, the IPNV VP2 gene obtained by amplification is cloned into a prokaryotic expression carrier plasmid pET30b, and expression is carried out in a colon bacillus BL21 strain to obtain the infectious pancreatic necrosis recombinant VP2 protein antigen. The antigen can overcome the potential hazards of toxin expulsion and toxin dispersion existing in an antigen in the traditional diagnosis method of infectious pancreatic necrosis, and can be used as a diagnostic antigen for disease surveillance.

Description

(1) Technical field [0001] The present invention relates to a biological preparation, and also relates to a preparation method of the biological preparation. Specifically, it relates to a fish infectious pancreatic necrosis recombinant VP2 protein antigen and a preparation method thereof. (2) Technical background [0002] Infectious pancreatic necrosis is a highly contagious and acute viral disease of salmonid fish caused by infectious pancreatic necrosis virus (IPNV). The typical symptoms are abnormal swimming and dark and dark body color of sick fish , abdominal distention, exophthalmos, hemorrhage and necrosis of subcutaneous, hepatopancreas and other visceral parenchymal organs. Eggs excrete pathogens and become a potential source of infection. Due to the widespread prevalence of the disease and the high morbidity and mortality rates, it has brought significant economic losses to the salmon and trout breeding industry in my country and the world. [0003] The traditio...

AI Technical Summary

Problems solved by technology

However, there is currently no effective preventive

In terms of diagnosis and detection, virus isolation and identification of suspicious disease materials are the most intuitive diagnostic methods for this disease, but virus isolation requires certain conditions and quite a long time. Therefore, this diagnostic method is time-consuming, laborious and cannot be quickly diagnosed. Disadvantages; the most commonly used rapid diagnosis methods for this disease are serological tests, fluorescent antibody tests and enzyme-linked immunosorbent assays, etc.

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