The invention relates to a blocking ELISA
antibody detection kit for porcinecircovirus type 2 and a preparation method of the blocking ELISA
antibody detection kit. The blocking ELISA
antibody detection kit for the porcinecircovirus type 2 comprises a coated plate, a PVC2
enzyme labelled antibody, sample
diluent, a concentrated washing solution (10*), a positive serum contrast, a negative serum contrast, TMB substrate
diluent and a stop solution, wherein the coated plate is prepared from a purified
porcine circovirus type 2 nucleocapsid
protein CapC
protein serving as a non-
antigen, and the PVC2
enzyme labelled antibody is prepared from
monoclonal antibody hybridoma which is labeled with
horse radish peroxidase and secretes PCV2. According to the preparation method, the CapC
protein is expressed by virtue of a
prokaryotic expression system, and the blocking ELISA is established by taking a
monoclonal antibody of PCV2 as the
enzyme labelled antibody; and the blocking ELISA
antibody detection kit has the characteristics of high specificity and sensitivity and can be applied to the detection of PCV2 antibodies of a
porcine serum sample.