5results about How to "Reduce non-specific reactions" patented technology

The invention discloses a portable immunochromatography device for detecting a bovine nodular skin disease virus neutralizing antibody and application of the portable immunochromatography device. The portable immunochromatography device comprises a sampling main body and a detection main body which are integrally connected front and back, and a spring sampler and a siphon are arranged in the sampling main body; a sample pretreatment cavity, a filter membrane, an antibody enrichment cavity and an immunochromatography detection cavity which are sequentially communicated from front to back are arranged in the detection main body, the siphon is communicated with the sample pretreatment cavity, a diluent storage cavity and a plastic needle are further arranged on the outer side of the sample pretreatment cavity, and the diluent storage cavity is communicated with the sample pretreatment cavity and is hermetically separated from the sample pretreatment cavity by a diaphragm; a liquid absorption pad and a chromatography pad are arranged in the antibody enrichment cavity, the liquid absorption pad is respectively connected with the filter membrane and the chromatography pad, an immunochromatography test strip is arranged in the immunochromatography detection cavity, and the chromatography pad is respectively connected with the liquid absorption pad and the immunochromatography test strip. The device integrates sampling and detection, is time-saving and accurate, reduces non-specific reaction, is simple and convenient to operate, and is suitable for large-scale detection and monitoring of the LSDV neutralizing antibody.

The invention relates to a biological reaction device, in particular to a high-pressure immunohistochemical system and method. The invention provides a high-pressure immunohistochemical system. The high-pressure immunohistochemical system comprises a thermal repair reaction device, an incubation device and a turnover mechanism, the thermal repair reaction device, the incubation device and the turnover mechanism are arranged; sample carriers can be accommodated in the thermal repair reaction device and the incubation device; and the overturning mechanism is used for overturning or / and moving the sample carrier. According to the method disclosed by the invention, the sample carrier is accurately positioned in different operation stages through the cooperation of the thermal repair reaction device, the incubation device and the turnover mechanism. And the overturning mechanism can ensure that the sample is covered in the liquid adding and incubation links, so that the loss and evaporation of the reagent are reduced.

This invention discloses a detection kit technology, specifically a detection kit and method for novoarginine, comprising a universal sample processing solution, a novoarginine-specific probe, fluorescently labeled streptavidin, washing solution, chromogenic substrate solution, stop solution, novoarginine standard, and a reaction microplate. The universal sample processing solution is composed of acetonitrile, potassium dihydrogen phosphate buffer, and disodium ethylenediaminetetraacetate in a volume ratio of 3:6:1, with the potassium dihydrogen phosphate buffer concentration being 0.1 mol / L and the disodium ethylenediaminetetraacetate concentration being 0.05 mol / L. The novoarginine-specific probe is a biotin-labeled novoarginine monoclonal antibody at a concentration of 1 mg / mL. The surface of the reaction microplate is coated with novoarginine hapten. This invention, by developing a universal sample processing solution adaptable to different matrix samples, eliminates the need for customized pretreatment procedures, significantly improving the versatility of the kit and solving the cumbersome problem of requiring separate processing for different samples.

The invention relates to a buffer solution. The buffer solution contains one of a non-mammalian protein, an enzymolysis product of the non-mammalian protein or a protein-free sealing agent. The invention also relates to a chemiluminiscence detection kit for the beta collagen degradation product and a preparation method of the chemiluminiscence detection kit. Through a large number of research experiments, the inventor finds that when the mammalian proteins such as BSA are applied to the beta collagen degradation product chemiluminiscence detection kit, the sensitivity and gradient are poor. One of non-mammalian proteins, enzymatic hydrolysate of the non-mammalian proteins or a protein-free sealing agent is adopted to replace BSA in the kit, non-specific reactions introduced in test reactions can be greatly reduced, the background of the item is effectively reduced, the gradient and sensitivity of the test are improved, and the stability of the test is enhanced.