Primer probe composition and kit for specificity detection and identification of neisseria meningitidis

A technology of Neisseria inflammatoryis and Neisseria bacterium, which is applied in the field of oligonucleotide sequence combination, can solve the problems of increased detection time cost and the inability to completely eliminate the risk of pathogenicity, and achieve the goal of saving detection costs Effect

Active Publication Date: 2012-08-22
JIANGSU UNINOVO BIOLOGICAL TECH
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Problems solved by technology

In the actual detection process, for a sample to be tested, if only detecting whether there is group A Neisseria meningitidis, or on the contrary, only detecting whether there is group C Ne

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  • Primer probe composition and kit for specificity detection and identification of neisseria meningitidis
  • Primer probe composition and kit for specificity detection and identification of neisseria meningitidis
  • Primer probe composition and kit for specificity detection and identification of neisseria meningitidis

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Embodiment Construction

[0029] Preferred implementations of the present invention will be described in detail below in conjunction with specific examples. It should be pointed out that the examples listed here are for the purpose of illustration only, and should not be construed as any limitation on the scope of the present invention. The reagent kits, buffers and other reagents used therein are only reagents specifically selected in this specific example. It should be understood that those skilled in the art can select corresponding reagents from other companies as needed to achieve the purpose of the present invention.

[0030]1. Design and synthesis of primers and TaqMan probes

[0031] Use the Blast tool to analyze the genome sequences of Neisseria meningitidis (common), Neisseria meningitidis group A and Neisseria meningitidis group C in Genbank and domestic and foreign literature, and select their stable conserved regions as the detection target sequence. Various serogroups of Neisseria menin...

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Abstract

The invention relates to a primer probe composition and a kit for specificity detection and identification of neisseria meningitidis, which adopts a real-time fluorescent polymerase chain reaction (PCR) method to specifically detect a nucleotide sequence composition existing in neisseria meningitidis nucleic acid in a sample and comprises the kit of the composition. By means of the sequence composition and the kit, whether the neisseria meningitidis nucleic acid exists or not in the sample can be rapidly detected, simultaneously whether the neisseria meningitidis nucleic acid belongs to the fashionable A group or C group in our country or not is identified. The limit of detection of the kit on the neisseria meningitidis (commonly used) is 200 copies per reaction system. The limit of detection on the neisseria meningitidis in the A group is 200 copies per reaction system, and the limit of detection on the neisseria meningitidis in the C group is 200 copies per reaction system. The whole reaction can be finished in 2 hours, and the primer probe composition and the kit have important application value in the fields of disease surveillance, clinical diagnosis and the like.

Description

technical field [0001] The invention relates to an oligonucleotide sequence combination for specifically detecting the nucleic acid of Neisseria meningitidis in a sample by using a real-time fluorescent PCR method, and a kit comprising the combination. Utilizing the sequence combination or kit of the present invention, it is possible to quickly detect whether there is Neisseria meningitidis nucleic acid in a sample, and at the same time identify whether it is the most popular group A or group C in my country. The detection limit of the kit for Neisseria meningitidis (common) is 200 copies per reaction system, the detection limit for group A Neisseria meningitidis is 200 copies per reaction system, and for group C Neisseria meningitidis The detection limit of bacteria was 200 copies per reaction system. The whole reaction can be completed within 2 hours, and has important application value in the fields of disease monitoring and clinical diagnosis. technical background [0...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12N15/11C12R1/36
Inventor 文锋
Owner JIANGSU UNINOVO BIOLOGICAL TECH
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