Bordetella pertussis and primer probe combination and kit for specific detection of bordetella parapertussis

A technology of parapertussis and primer combination, which is applied in the field of oligonucleotide sequence combination, can solve the problems of underestimation of pertussis incidence and lack of specific laboratory detection methods, and achieve the effect of saving detection costs

Active Publication Date: 2012-08-15
JIANGSU UNINOVO BIOLOGICAL TECH
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Problems solved by technology

[0005] The incidence of whooping cough may be underestimated due to the lac

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  • Bordetella pertussis and primer probe combination and kit for specific detection of bordetella parapertussis
  • Bordetella pertussis and primer probe combination and kit for specific detection of bordetella parapertussis
  • Bordetella pertussis and primer probe combination and kit for specific detection of bordetella parapertussis

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Embodiment Construction

[0027] The preferred embodiments of the present invention will be described in detail below with reference to specific examples. It should be pointed out that the embodiments listed here are only for illustrative purposes and should not be interpreted as any limitation on the scope of the present invention. The reagent kits, buffers and other reagents used therein are only the reagents specifically selected in the specific embodiment. It should be understood that those skilled in the art can select corresponding reagents from other companies as needed to achieve the purpose of the present invention.

[0028] 1. Design and synthesis of primers and TaqMan probes

[0029] Use the Blast tool to analyze the genome sequences of all Bordetella pertussis and Bordetella parapertussis in Genbank and domestic and foreign literature, and select their stable conserved regions as the detection target sequences, and target these two detections Target sequence design and synthesis of multiple set...

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Abstract

The invention relates to an oligonucleotide sequence combination for specific detection of bordetella pertussis and bordetella parapertussis nucleic acids existing in a sample with the adoption of fluorescent PCR (polymerase chain reaction), and a kit containing the combination. The kit can sensitively detect the bordetella pertussis nucleic acid and the bordetella parapertussis nucleic acid existing in the sample with a detection lower limit of 20 copies in each reaction system, and has important application value in the fields such as disease surveillance and clinical diagnosis.

Description

Technical field [0001] The invention relates to an oligonucleotide sequence combination for specifically detecting the presence of Bordetella pertussis and Bordetella parapertussis nucleic acids in a sample by using a fluorescent PCR technology, and a kit comprising the combination. technical background [0002] Whooping cough is an acute respiratory infection caused by Bordetella pertussis. Its clinical features are paroxysmal spasmodic cough accompanied by a deep "cock crow"-like inspiratory roar. If it is not treated promptly and effectively, the course of the disease can reach several months. The disease is very contagious. The younger the child is, the more severe the condition, and death due to concurrent pneumonia and encephalopathy. [0003] Patients with whooping cough is the only source of infection. They are infectious from 1 to 2 days at the end of the incubation period to 6 weeks after the onset of illness, with the most infectious in the first 1 to 3 weeks. When a p...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12N15/11C12R1/01
Inventor 文锋
Owner JIANGSU UNINOVO BIOLOGICAL TECH
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