Primer and probe combination and kit for specifically detecting streptococcus pneumoniae and haemophilus influenzae

A technology of Haemophilus influenzae and Streptococcus pneumoniae, applied in the field of oligonucleotide sequence combination, can solve the problems of insufficient primer specificity, false negative results, false positive results, etc., and achieve the effect of saving detection costs

Active Publication Date: 2012-09-12
JIANGSU UNINOVO BIOLOGICAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

If the primers are not specific enough, it may lead to false

Method used

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  • Primer and probe combination and kit for specifically detecting streptococcus pneumoniae and haemophilus influenzae
  • Primer and probe combination and kit for specifically detecting streptococcus pneumoniae and haemophilus influenzae
  • Primer and probe combination and kit for specifically detecting streptococcus pneumoniae and haemophilus influenzae

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Embodiment Construction

[0027] Preferred implementations of the present invention will be described in detail below in conjunction with specific examples. It should be pointed out that the examples listed here are for the purpose of illustration only, and should not be construed as any limitation on the scope of the present invention. The reagent kits, buffers and other reagents used therein are only reagents specifically selected in this specific example. It should be understood that those skilled in the art can select corresponding reagents from other companies as needed to achieve the purpose of the present invention.

[0028] 1. Design and synthesis of primers and TaqMan probes

[0029] Use the Blast tool to analyze all the genome sequences of Streptococcus pneumoniae and Haemophilus influenzae in Genbank and domestic and foreign literatures, select their stable conserved regions as detection target sequences, and design and synthesize polynucleotides for these two detection target sequences. Se...

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Abstract

The invention relates to an oligonucleotide sequence combination for specifically detecting existence of streptococcus pneumoniae and haemophilus influenzae nucleic acids in samples by adopting fluorescent PCR (polymerase chain reaction) technology and a kit comprising the combination. The kit can sensitively detect and identify the streptococcus pneumoniae and haemophilus influenzae nucleic acids in the samples, has limits of detection of 20 copies per reaction system and has important application values in the fields such as disease surveillance and clinical diagnosis.

Description

technical field [0001] The invention relates to an oligonucleotide sequence combination for specifically detecting nucleic acids of Streptococcus pneumoniae and Haemophilus influenzae in a sample by using fluorescent PCR technology, and a kit comprising the combination. technical background [0002] The pathogens of bacterial pneumonia vary greatly due to host age, concomitant diseases, and immune function status. In terms of acquisition methods, the common pathogens of community-acquired pneumonia are Streptococcus pneumoniae and Haemophilus influenzae. [0003] Streptococcus pneumoniae is a Gram-positive coccus and is the most important pathogen causing bacterial pneumonia, accounting for about 2 / 3 of it. It can occur throughout the year, with a high incidence in winter and spring. Haemophilus influenzae is a small Gram-negative bacillus that is highly prevalent in children aged 4-18 months and can cause bacterial pneumonia and bacterial meningitis. According to WTO stat...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12Q1/14C12Q1/04C12N15/11G01N21/64
Inventor 文锋其他发明人请求不公开姓名
Owner JIANGSU UNINOVO BIOLOGICAL TECH
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