A kit for hepatocellular carcinoma auxiliary diagnosis or disease surveillance and applications

A technology for auxiliary diagnosis and disease monitoring, applied in the field of tumor molecular biology, can solve the problems of lncRNAs lack of protein coding ability, etc., and achieve important promotion and application prospects and far-reaching clinical significance

Inactive Publication Date: 2017-12-15
CENT SOUTH UNIV
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Problems solved by technology

It is currently believed that lncRNAs lack protein coding ability and can be localized in the nucleus and cytoplasm

Method used

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  • A kit for hepatocellular carcinoma auxiliary diagnosis or disease surveillance and applications
  • A kit for hepatocellular carcinoma auxiliary diagnosis or disease surveillance and applications
  • A kit for hepatocellular carcinoma auxiliary diagnosis or disease surveillance and applications

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Experimental program
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Embodiment Construction

[0019] Electronic clone screening and PCR amplification of full-length novel genes

[0020] Five EST sequences with unknown functions were obtained by electronic cloning technology, and the fifth sequence was initially selected for follow-up experiments. According to the conventional RACE method, Nest-PCR amplification was carried out, and the full-length sequence of the HCCL5 gene was successfully obtained after splicing the 3'RACE and 5'RACE amplification results; bioinformatics predicted that it was a long-chain non-coding RNA, so it was initially named lncRNA HCCL5, the sequence is as SEQ ID 4 in the sequence listing.

[0021] Design of in situ hybridization oligonucleotide probes for lncRNA HCCL5

[0022] In order to detect the expression of long noncoding RNA gene HCCL5 by in situ hybridization, we designed three oligonucleotide probes for in situ hybridization detection of lncRNA HCCL5 expression.

[0023] The oligonucleotide probes used to detect the expression of ln...

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Abstract

A kit for hepatocellular carcinoma auxiliary diagnosis or disease surveillance is disclosed. The kit includes an oligonucleotide probe detecting expression of a long-chain non-coding RNA gene that is HCCL5 by adopting in-situ hybridization. The kit provides a powerful molecular biological tool for hepatocellular carcinoma auxiliary diagnosis and disease surveillance, and has profound clinical significance and an important popularization and application prospect.

Description

technical field [0001] The invention belongs to the field of tumor molecular biology, and in particular relates to a kit for auxiliary diagnosis or disease monitoring of hepatocellular carcinoma and its application. Background technique [0002] Hepatocellular carcinoma (hepatocellular carcinoma, HCC) is a common malignant tumor. According to a report in 2012, it is estimated that there are 782,000 new cancer cases in the world in one year (50% in China alone); It is also the third leading cause of cancer death worldwide, with nearly 745,000 patient deaths in 2012. Despite the increasing prevalence of HCC, effective treatments are still lacking. At the same time, liver cancer is a devastating disease, and the 5-year overall survival (OS) rate in developed and developing countries is usually <20%; and the ratio of mortality to incidence is 0.95, which again shows that HCC The death rate of patients is very high and the survival rate is low. Hepatocellular carcinoma is a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68
Inventor 彭丽李官成袁小青蒋斌元张朝阳李文玲刘朝阳孙瑞利潘曦张雅琴
Owner CENT SOUTH UNIV
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