Immunochromatographic kit for quantitative detection of RV (rubella virus) IgG (immunoglobulin G) antibody through quantum dots

A quantitative detection and immunochromatography technology, applied in the field of immunoassay, can solve the problems of poor label stability, low sensitivity, long reaction time, etc., and achieve the effects of convenient monitoring, high sensitivity and simple operation.

Inactive Publication Date: 2015-11-18
AUTOBIO DIAGNOSTICS CO LTD
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  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

There are no obvious symptoms during the secondary infection, and only a moderate increase in the serum level of specific IgG can be detected
The ELISA method uses horseradish peroxidase (horseradishperoxidase, abbreviated as HRP) or alkaline phosphatase to label the antibody, and catalyzes the color change of the substrate. It has the characteristics of simple operation and long stable period of the reagent, but the ELISA method requires special Equipment, the reaction time is relatively long, and the detection steps are complicated, including: sample addition, in

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  • Immunochromatographic kit for quantitative detection of RV (rubella virus) IgG (immunoglobulin G) antibody through quantum dots
  • Immunochromatographic kit for quantitative detection of RV (rubella virus) IgG (immunoglobulin G) antibody through quantum dots
  • Immunochromatographic kit for quantitative detection of RV (rubella virus) IgG (immunoglobulin G) antibody through quantum dots

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Embodiment Construction

[0019] The present invention will be described in more detail below in combination with specific embodiments.

[0020] Quantum dots of the present invention quantitatively detect the immunochromatography kit of RV virus IgG antibody, comprise detection test strip, serial standard product and sample diluent, such as figure 1 As shown, the detection test strip is composed of absorbent paper 2, nitrocellulose membrane 3, binding pad 4, and sample pad 5 that are pasted on the PVC liner 1 in sequence: the nitrocellulose membrane detection line 6 is coated with the purified antigen of RV virus The quality control line 7 is coated with goat anti-mouse IgG polyclonal antibody; the binding pad 4 is sprayed with quantum dot-labeled mouse anti-human IgG monoclonal antibody solution on polyester glass fiber and dried to a solid state. The series of standards in this kit include negative calibrator and high, medium and low positive calibrator, the negative calibrator includes PBS buffer, t...

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Abstract

The invention discloses an immunochromatographic kit for quantitative detection of an RV (rubella virus) IgG (immunoglobulin G) antibody through quantum dots. The immunochromatographic kit comprises a detection strip, series of standards and a sample diluent, wherein the detection strip comprises absorbent paper, a nitrocellulose membrane, a conjugate pad and a sample pad which are bonded to a liner plate sequentially; a detection zone of the nitrocellulose membrane is coated with an RV purified antigen, and a quality control zone of the nitrocellulose membrane is coated with a goat-anti-mouse IgG polyclonal antibody; the conjugate pad is formed by spraying a quantum dot labeled mouse-anti-human IgG monoclonal antibody solution onto a polyester glass fiber and drying the polyester glass fiber into a solid state. The immunochromatographic kit has the advantages as follows: a quantum dot quantification technology and immunochromatography are combined, the operation is simple and rapid, and limit of the sample number is avoided, the sensitivity is higher than that of a colloidal gold immunochromatographic method, RV IgG antibodies in human whole blood, serum and blood plasma can be detected quantitatively, and RV-IgG antibody level detection can be performed on progestational women more conveniently.

Description

technical field [0001] The invention relates to the field of immunoanalysis, in particular to an immunochromatography kit for quantitative detection of RV virus IgG antibody by quantum dots. Background technique [0002] Rubella virus (rubellavirusRV) belongs to the Togaviridae Rubellavirus genus in the herpes-like virus, is the pathogen that causes rubella, is an RNA virus, and humans are its only host. The virus is transmitted through the respiratory tract, and the incubation period is about 2 to 3 weeks. After the virus multiplies, it enters the bloodstream and causes viremia. The clinical manifestations of rubella are similar to measles, but the symptoms are milder than those of measles infection, and there are fewer complications. Adults infected by rubella virus have symptoms like common colds. First, there are general symptoms of colds and swollen lymph nodes behind the ears and under the pillow, followed by facial symptoms. Latent erythematous papules appear and qui...

Claims

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Application Information

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IPC IPC(8): G01N21/64G01N33/577
Inventor 李奎李静辉陶占领王菁付青山刘功成付光宇吴学炜
Owner AUTOBIO DIAGNOSTICS CO LTD
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