Immunochromatographic kit for quantitative detection of RV (rubella virus) IgG (immunoglobulin G) antibody through quantum dots

Abstract

The invention discloses an immunochromatographic kit for quantitative detection of an RV (rubella virus) IgG (immunoglobulin G) antibody through quantum dots. The immunochromatographic kit comprises a detection strip, series of standards and a sample diluent, wherein the detection strip comprises absorbent paper, a nitrocellulose membrane, a conjugate pad and a sample pad which are bonded to a liner plate sequentially; a detection zone of the nitrocellulose membrane is coated with an RV purified antigen, and a quality control zone of the nitrocellulose membrane is coated with a goat-anti-mouse IgG polyclonal antibody; the conjugate pad is formed by spraying a quantum dot labeled mouse-anti-human IgG monoclonal antibody solution onto a polyester glass fiber and drying the polyester glass fiber into a solid state. The immunochromatographic kit has the advantages as follows: a quantum dot quantification technology and immunochromatography are combined, the operation is simple and rapid, and limit of the sample number is avoided, the sensitivity is higher than that of a colloidal gold immunochromatographic method, RV IgG antibodies in human whole blood, serum and blood plasma can be detected quantitatively, and RV-IgG antibody level detection can be performed on progestational women more conveniently.

Description

technical field [0001] The invention relates to the field of immunoanalysis, in particular to an immunochromatography kit for quantitative detection of RV virus IgG antibody by quantum dots. Background technique [0002] Rubella virus (rubellavirusRV) belongs to the Togaviridae Rubellavirus genus in the herpes-like virus, is the pathogen that causes rubella, is an RNA virus, and humans are its only host. The virus is transmitted through the respiratory tract, and the incubation period is about 2 to 3 weeks. After the virus multiplies, it enters the bloodstream and causes viremia. The clinical manifestations of rubella are similar to measles, but the symptoms are milder than those of measles infection, and there are fewer complications. Adults infected by rubella virus have symptoms like common colds. First, there are general symptoms of colds and swollen lymph nodes behind the ears and under the pillow, followed by facial symptoms. Latent erythematous papules appear and qui...

AI Technical Summary

Problems solved by technology

There are no obvious symptoms during the secondary infection, and only a moderate increase in the serum level of specific IgG can be detected

The ELISA method uses horseradish peroxidase (horseradishperoxidase, abbreviated as HRP) or alkaline phosphatase to label the antibody, and catalyzes the color change of the substrate. It has the characteristics of simple operation and long stable period of the reagent, but the ELISA method requires special Equipment, the reaction time is relatively long, and the detection steps are complicated, including: sample addition, incubation, plate washing, substrate addition, color development, termination, detection and other procedures. The detection time exceeds 90 minutes, which is not conducive to the development of primary hospitals and outpatient clinics.

Colloidal gold immunochromatography is widely used at present, and the operation is simple and fast. However, in this method, the stability of the markers is poor, the color is single, the sensitivity is low, and the background interference of the matrix is ​​large, and it can only be detected qualitatively. do accurate quantification

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