Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Recombinant rubella virus E1 protein and uses thereof

A rubella virus and protein technology, applied in the field of genetic engineering, can solve the problems of difficult separation and purification of antigen component E1, no research and development of commercial diagnostic kits, and difficulty in separation of antigen components, so as to meet the needs and sensitivity of clinical diagnosis. High, specific effect

Inactive Publication Date: 2009-04-08
吴丽霞 +2
View PDF0 Cites 8 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the separation of the E1 / E2 / C antigenic components of rubella virus is relatively difficult, especially the separation and purification of the main antigenic component E1, which is basically not used in the research and development of commercial diagnostic kits

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Recombinant rubella virus E1 protein and uses thereof
  • Recombinant rubella virus E1 protein and uses thereof
  • Recombinant rubella virus E1 protein and uses thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] The preparation of embodiment 1 recombinant rubella virus E1 protein

[0034] The amino acid sequence of the recombinant rubella virus E1 protein is designed according to the amino acid sequence of RV envelope glycoprotein E1 (glycoprotein E1), and the sequence is 203-295 amino acids of the amino acid sequence of RV envelope glycoprotein E1 (glycoprotein E1).

[0035] 1.1 Synthesis of recombinant DNA of rubella virus E1 protein

[0036] The amino acid sequence of RV envelope glycoprotein E1 (glycoprotein E1) is analyzed by computer, combined with the confirmed antigenic epitope, and a part of the sequence with strong antigenicity is selected.

[0037] The complete amino acid sequence of RV envelope glycoprotein E1 (glycoprotein E1) is as follows:

[0038] EEAFTYLCTAPGCATQAPVPVRLAGVRFESKIVDGGCFAPWDLEATGACICEIPTDVSCEGLGAWVPAAPCARIWNGTQRACTFWAVNAYSSGGYAQLASYFNPGGSYYKQYHPTACEVEPAFGHSDAACWGFPTDTVMSVFALASYVQHPHKTVRVKFHTETRTVWQLSVAGVSCNVTTEHPFCNTIMPHGDPGQLEV NQQSRWGLGSPNCHG...

Embodiment 2

[0079] Example 2 Preparation and Performance Test of the Kit for Detecting Rubella Virus IgM Antibody Infection

[0080] 2.1 Preparation of a kit for detecting rubella virus IgM antibody infection

[0081] The recombinant rubella virus E1 protein prepared in Example 01 was used as the substrate for the preparation of the enzyme-labeled antigen of the kit, and the RVIgM antibody was detected by ELISA.

[0082] The kit was developed and used as follows:

[0083] 1) The principle of the kit: this strain uses anti-human IgM (μ chain) coated microwell plate, horseradish peroxidase (HRP) labeled recombinant rubella virus E1 as tracer, TMB chromogenic system, application capture The principle of the method is to detect the anti-rubella virus IgM antibody in human serum or plasma.

[0084] 2) The main components of the kit:

[0085] a) Pre-coated plates:

[0086] b) Coating solution: 0.05mol / l, carbonate buffer system with pH9.6

[0087] c) Blocking solution: composed of 0.01mol / ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a recombinant rubella virus E1 protein and an application thereof. The adopted technical proposal is as follows: the amino acid sequence of the recombinant rubella virus E1 is shown in SEQ ID NO: 2, a reagent kit which is prepared by the recombinant rubella virus E1 protein and used for detecting the rubella virus IgM antibody infection is adopted, compared with the similar reagent kits on the market, the reagent kit has the advantages of strong specificity, high sensitivity, and the like, thereby well meeting the needs of clinical diagnosis of rubella virus infection. The invention further provides the application of the recombinant rubella virus E1 protein in the preparation of monoclonal antibodies, polyclonal antibodies and protein chips.

Description

Technical field: [0001] The invention relates to the field of genetic engineering, in particular to a recombinant rubella virus E1 protein and its application. Background technique: [0002] Rubella virus (rubella virus, RV) RV is the only member of the Rubellavirus genus in the Togaviridae family, and humans are its only natural host. Rubella itself is not serious, and acquired infection mainly manifests as acute fever, submandibular lymph node enlargement and other symptoms. The greatest threat to RV is its teratogenicity. Pregnant women infected with RV can cause congenital damage to the fetus, especially if infected with RV in the first 3 months of pregnancy, it can infect the fetus through the placenta, resulting in miscarriage, stillbirth, or a series of organ malformations in the newborn, that is, congenital rubella syndrome. Establishing a rapid and accurate detection method for RV infection is the key to preventing RV infection. [0003] At present, the diagnosti...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12N15/40C07K14/19C12N15/63C12N1/21G01N33/68C07K16/10C12R1/19
Inventor 吴丽霞赵玉红于庭包洪王贵全李凤娣姜禾董亚俊徐田雪
Owner 吴丽霞
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com