Recombinant rubella virus protein and uses thereof

A rubella virus and protein technology, applied in the fields of genetic engineering, vaccine development and diagnostic reagents

Active Publication Date: 2009-08-19
北京英诺特生物技术股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, most of the RV protein antigens used in detection kits are derived from virus culture or recombinantly expressed single protein fragments, or due to low purity and poor specificity of protein antigens, false positives are caused and the detection specificity is reduced.
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Method used

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  • Recombinant rubella virus protein and uses thereof
  • Recombinant rubella virus protein and uses thereof
  • Recombinant rubella virus protein and uses thereof

Examples

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Embodiment Construction

[0017] The invention discloses a nucleotide sequence encoding rubella virus protein as shown in sequence 1, a method for preparing rubella virus protein by using the nucleotide sequence, and the rubella virus containing the amino acid sequence shown in sequence 2 prepared by the method The protein, as well as the composition and kit containing the protein, also disclose their application in detecting human rubella virus infection. In addition, the rubella virus protein of the present invention and the nucleic acid of the present invention can also be used in the preparation of rubella virus vaccines.

[0018] One embodiment of the present invention relates to a nucleotide sequence as shown in Sequence 1 encoding a rubella virus protein. The bases are numbered in the conventional 5' to 3' order.

[0019] The nucleotide sequence shown in Sequence 1 can be prepared by conventional methods in the art, preferably based on the DNA sequence of the target fragment, that is, the cDNA ...

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Abstract

The invention relates to a rubella virus protein for clinical diagnosis of rubella virus infection and a nucleotide sequence encoding the protein. The protein comprises plasmids of the nucleotide sequence and procaryotic host cells. The invention also comprises a method for preparing the protein and application thereof.

Description

technical field [0001] The invention relates to the fields of genetic engineering, vaccine development, diagnostic reagents and the like, in particular to a recombinant rubella virus protein and its application. Background technique [0002] Rubella virus is the only member of the genus Rubellavirus in the Togaviridae family. The spherical rubella virus (RUBELLA VIRUS, RUV) is the pathogen of rubella, and humans are its only host. Rubella is an acute respiratory infectious disease with fever, respiratory tract sputum and rash as the main symptoms. Rubella virus infection in pregnant women, especially in early pregnancy, can lead to fetal congenital rubella syndrome (CONGENITAL RUBELLASYNDROME, CRS). Rubella and CRS occur all over the world, and the infection rate is high in Asia. After pregnant women suffer from rubella, especially within the first 3 to 4 months of pregnancy, rubella virus can invade the placenta and infect the fetus, causing congenital rubella as high as ...

Claims

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Application Information

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IPC IPC(8): C12N15/40C12N15/63C12N15/70C12N15/74C12P21/02C12Q1/70C12Q1/68A61K39/20A61K48/00A61P31/14C12R1/93C12R1/19
Inventor 陈廷友孔祥菊王健张小刚
Owner 北京英诺特生物技术股份有限公司
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