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Four-color TORCH (Toxo, others, RV, CMV and HSV) fluorescent detection kit

A fluorescence detection and kit technology, applied in the field of molecular biology, can solve the problems of long time-consuming, low amplification sensitivity, etc., achieve high detection throughput and reduce screening costs

Inactive Publication Date: 2017-08-18
GUANGZHOU HEAS BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The present invention mainly provides a four-color TORCH fluorescence detection technology, which can solve the problems of low amplification sensitivity and long time consumption in the prior art.

Method used

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  • Four-color TORCH (Toxo, others, RV, CMV and HSV) fluorescent detection kit
  • Four-color TORCH (Toxo, others, RV, CMV and HSV) fluorescent detection kit
  • Four-color TORCH (Toxo, others, RV, CMV and HSV) fluorescent detection kit

Examples

Experimental program
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Effect test

Embodiment 1

[0067] Example 1 The detection operation method of the four-color TORCH fluorescence detection kit:

[0068] 1. Sample processing and DNA extraction

[0069] Add 50µl virus extract to 50ul serum or plasma sample, shake for 60 seconds, fully suspend the cell pellet, and set aside.

[0070] Nucleic acid extraction can also be performed using other known methods;

[0071] Quality control handling

[0072] Take the centrifuge tubes containing the positive quality control and negative quality control, centrifuge at 10,000rpm for 30 seconds, and set aside;

[0073] Nucleic acid amplification reagent preparation (reagent preparation area, operation on ice)

[0074] 1) Take out the TORCH PCR reaction tube from the kit and centrifuge briefly for later use;

[0075] 2) Add 20µl of the extracted nucleic acid of the sample to be tested (if the sample is less than 20µl, please make up 20µl with sterilized purified water before adding the sample), 20µl of the negative quality control su...

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Abstract

The invention discloses a four-color TORCH (Toxo, others, RV, CMV and HSV) fluorescent detection kit, comprising a hot start Taq enzyme system, primers, a fluorescent probe, a negative control and a positive control; the fluorescent probe is a TORCH-specific MGB (minor groove binder) probe. The four-color TORCH fluorescent detection kit is suitable for qualitatively detecting toxoplasma, rubella virus, cytomegalovirus, and herpes simplex virus type I and type II in serum and plasma samples. The four-color TORCH fluorescent detection kit detects toxoplasma, rubella virus, cytomegalovirus, and herpes simplex virus type I and type II in a same tube amplification system by means of multiple fluorescence PCR (polymerase chain reaction) technique. The four-color TORCH fluorescent detection kit has high detection flux and has greatly reduced screening cost.

Description

technical field [0001] The invention belongs to the field of molecular biology and relates to a TORCH fluorescence detection kit, in particular to a four-color TORCH fluorescence detection kit. Background technique [0002] TORCH refers to the pathogen that can cause congenital intrauterine infection and perinatal infection and cause perinatal malformation. The microorganisms that can cause intrauterine and fetal infection before and during pregnancy mainly include Toxoplasma gondii (Toxo) and rubella virus. (Rubella Virus, RV), cytomegalovirus (Cytomegalovirus, CMV), herpes simplex virus type I and type II (Herpes Simplex Virus, HSV), and their harm to eugenics is relatively serious. In 1971, Nalmias specially coined the word "TORCH" based on the first letter of the English names of these microorganisms, To stands for Toxoplasma; R stands for Rubella Virus; C stands for Cytomegalovirus; H stands for Herpes Simplex Type I and Type II Virus. TORCH infection is called "ToRCH...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/70C12Q1/68C12Q1/04C12R1/93C12R1/90
CPCC12Q1/6893C12Q1/701C12Q1/705C12Q2600/16
Inventor 刘淑园陈华云肖湘文丁渭
Owner GUANGZHOU HEAS BIOTECH CO LTD
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