Primer probe combination for specific detection of measles virus and rubella virus and kit

A measles virus, rubella virus technology, applied in the field of oligonucleotide sequence combination, can solve the problems of insufficient primer specificity, false positive results, false negative results, etc., and achieve the effect of saving detection costs

Active Publication Date: 2012-08-15
JIANGSU UNINOVO BIOLOGICAL TECH
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

If the primers are not specific enough, it may lead to false

Method used

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  • Primer probe combination for specific detection of measles virus and rubella virus and kit
  • Primer probe combination for specific detection of measles virus and rubella virus and kit
  • Primer probe combination for specific detection of measles virus and rubella virus and kit

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[0027] Preferred implementations of the present invention will be described in detail below in conjunction with specific examples. It should be pointed out that the examples listed here are for the purpose of illustration only, and should not be construed as any limitation on the scope of the present invention. The reagent kits, buffers and other reagents used therein are only reagents specifically selected in this specific example. It should be understood that those skilled in the art can select corresponding reagents from other companies as needed to achieve the purpose of the present invention.

[0028] 1. Design and synthesis of primers and TaqMan probes

[0029] Use the Blast tool to analyze all the measles virus and rubella virus genome sequences in Genbank and domestic and foreign literature, select their stable conserved regions as detection target sequences, and design and synthesize multiple sets of primers and Probes (see Table 1). The primers and probes were synt...

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Abstract

The invention relates to an oligonucleotide sequence combination for specific detection of nucleic acid of measles virus and rubella virus in a sample by utilizing a fluorescent PCR (polymerase chain reaction) technology, as well as a kit including the combination. The kit can sensitively detect and identify the nucleic acid of the measles virus and the rubella virus in the sample, the detection lower limit is 20 copies per reaction system, and the kit further has an important application value in the fields of disease monitoring, clinical diagnosis and the like.

Description

technical field [0001] The invention relates to an oligonucleotide sequence combination for specifically detecting measles virus and rubella virus nucleic acid in a sample by using fluorescent PCR technology, and a kit comprising the combination. technical background [0002] Measles is a systemic eruptive acute respiratory infectious disease caused by measles virus. It is highly contagious and easy to cause outbreaks. It is one of the serious infectious diseases that endanger children's life and health. Measles remains the deadliest disease among vaccine-preventable diseases. WHO has listed measles as the next disease to be eliminated in polio-free areas. All member countries in the Western Pacific Region of WHO have committed to eliminate measles in 2012. The Ministry of Health of my country has organized the "National Measles Elimination Action Plan 2006-2012". In recent years, with the development of planned immunization work, after the general application of live atten...

Claims

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Application Information

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IPC IPC(8): C12Q1/70C12Q1/68C12N15/11C12R1/93
Inventor 文锋
Owner JIANGSU UNINOVO BIOLOGICAL TECH
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