Method for detecting rubella virus, quantum dot-labeled immunochromatographic test paper and preparation method thereof

An immunochromatographic test strip and rubella virus technology, applied in the field of medical immunodetection, can solve the problems of low accuracy and low sensitivity, and achieve the effects of narrow emission peak, good luminescence stability and symmetrical peak shape.
CN103529211AActive Publication Date: 2014-01-22魏县聚邦新材料科技有限公司
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
魏县聚邦新材料科技有限公司
Publication Date
2014-01-22

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Abstract

The invention relates to a medimmune inspection method, and particularly relates to quantum dot-labeled immunochromatographic test paper, and a method for detecting rubella virus by adopting an immunological method. According to the quantum dot-labeled immunochromatographic test paper, a glass cellulose membrane A, a quantum dot-labeled rubella virus IgG monoclonal antibody glass cellulose membrane B, a cellulose nitrate membrane and absorbent paper are sequentially bonded on a plastic board from bottom to top, wherein a rubella virus polyclonal antibody and a rabbit-anti-mouse secondary antibody are arranged on one end of the cellulose nitrate membrane so as to form an inspection strip T and a quality control strip C; a quantum dot-labeled rubella virus IgG monoclonal antibody is located at the other end of the glass cellulose membrane B and corresponds to the inspection strip T and the quality control strip C, and the quantum dot-labeled rubella virus IgG monoclonal antibody is located at one end of a sample feeding point. The inspection sensitivity of the method is higher than of the currently used method by about 1000 times.
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Description

technical field

[0001] The invention relates to a medical immunological detection method, in particular to a method for immunologically detecting rubella virus by using quantum dot-labeled immunochromatographic test paper. Background technique

[0002] Rubella virus is an RNA virus belonging to the Togavirus genus. Rubella virus polyclonal antibody structure is quite stable, there is only one polyclonal antibody type, no subtype. It only infects humans and can grow in rabbit kidney, suckling vole kidney and green monkey kidney cells. The shape is rough and spherical, with a diameter of 50-70nm. It consists of a single-stranded RNA genome and a lipid shell, which contains an electron-dense core and is covered with two layers of loose coats. The virus is heat-labile and quickly loses its activity at 37°C and room temperature. It is cold-resistant and can be stored for a short period of time at -20°C and relatively stable for several months at -60°C. Viability outside the hu...

Claims

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