43results about How to "To achieve the purpose of quantitative detection" patented technology

A method adopted by an electrochemical biosensor based on sulfonic functionalized grapheme-chitosan to detect methyl parathion belongs to the technical field of electrochemical sensors and is used for detecting methyl parathion residual quantity in vegetables, fruits and products thereof. The basis of the invention is that methyl parathion can play an inhibition role on acetylcholinesterase, and the acetylcholinesterase can catalyze acetylthiocholine chloride to achieve electrochemical reaction of hydrosulfuryl acetylcholine. Sulfonic functionalized grapheme-chitosan is decorated on the surface of a bare glass carbon electrode, so that not only acetylcholinesterase is effectively fixed on the electrode, but also an electrochemical signal is amplified; and the methyl parathion residual quantity is related with electrochemical summit current, so that a standard curve for quantified detection of methyl parathion is built. The invention aims to provide the methyl parathion quantified detection method with high sensitivity and strong operability and develops the application of novel material graphene.

The invention provides a gene expression method for simultaneously detecting 11 sports-related genes and 4 internal reference genes in human blood. The expression conditions of the 15 genes are detected simultaneously in the same reaction system by applying a multi-PCR, universal primer and nucleic acid hybridization method. The 11 sports-related genes are ciliary neurotrophic factor (CNTF) gene, beta2-adrenergic receptor (beta2-AR) gene, leptin receptor gene, transforming growth factor-beta1 (TGF-beta1) gene, mitochondrial uncoupling protein 2 gene, phospholipase C-gamma1 gene, tumor necrosis factor (TNF) gene, haptoglobin gene, RAD1 homologue gene, hypoxia inducible factor-1alpha (HIF-1alpha) gene and insulin-like growth factor-1 (IGF-1) of a human; and the 4 internal reference genes are histone deacetylase gene, glyceraldehyde-3-phosphate dehydrogenase gene, 18s-rRNA gene and beta-actin gene. Compared with the conventional real-time fluorescent quantitative polymerase chain reaction (PCR) detection method, the method of the invention has the advantages of high throughput, quickness, high automation degree, moderate cost, high accuracy and sensitivity and the like.

The invention discloses a method and a system for detecting local impedance change of objects based on controlled knocking and a classifier. Classification is carried out by that an object has different local impedances and different response signals after being knocked and by the combination with a pattern recognition method. The method and the system not only can be used for detecting bolt screwing moment, but also can be used for detecting the damage status of composite material. The device adopted by the method is simpler, the method is simple and practical, the detection result is accurate, and multiclass classification can be realized.

The invention discloses a method for detecting the bending strength of a battery. The method comprises the following steps: firstly, supporting a battery to be detected by two supporting points with the span of L; then applying a load P to a middle position between the two supporting points along the direction which is vertical to the surface of the battery to be detected; recording the size P1 of the load P when the battery to be detected has the deformation with the route a or recording the size P2 of the load P when the battery is damaged; and finally, substituting obtained data into a formula to calculate the value of the bending strength to realize the aim of quantitatively detecting the bending strength of the battery. With the adoption of the method disclosed by the invention, a specific numerical value for evaluating the bending strength of the battery can be obtained; a detection result is objective and the different detection results have the comparability. The invention further provides a device for detecting the bending strength of the battery.

The invention provides gold labeled immunochromatographic strip assay semiquantitative detection test paper for detecting dienestrol. The test paper is characterized in that a sample absorption pad, a colloid gold label pad, a chromatography pad and a water absorption pad are pasted on the back lining of the test paper; and a detection reaction pad is covered by dienestrol antigen serving as a detection line and second antibody serving as a quality control line at the same time. The semiquantitative detection test paper has the advantages that the test paper has strong specificity and can realize semiquantitative detection; the test paper can be used at a temperature of between 4 and 40 DEG C and can obtain a result after 5 minutes; and the test paper is suitable for quickly detecting dienestrol drugs in animal-derived foodstuff samples by sanitation departments, quality inspection departments, customs and livestock farms and foodstuff enterprises.

The invention provides a method for detecting Vibrio parahemolyticus based on aptamer identification-molecular motor biosensing. According to the method, the interior of a chromatophore is labeled with a pH-sensitive fluorescent dye F1300 so that a signal probe is obtained, an aptamer specifically recognizing Vibrio parahemolyticus is used as a recognition probe, and an epsilon subunit antibody-biotin-avidin-end 5' biotinylated Vibrio parahemolyticus aptamer system connects the recognition probe and an F0F1-ATPase molecule motor so that the F0F1-ATPase molecule motor aptamer sensing system isconstructed. The method has the lowest detection limit of 15cfu/mL to Vibrio parahemolyticus, can be used for detection of Vibrio parahemolyticus in a salmon sample and can produce an accurate and reliable result.

The invention provides a method of detecting vibrio parahemolyticus based on an aptamer recognizing quantum dot labelled molecular motor biosensor. The aptamer of vibrio parahemolyticus is specifically recognized as a recognizing probe by labeling a pH sensitive quantum dot fluorescent nanomaterial to the surface of a color carrier. An F0F1 ATPas molecular motor aptamer sensing system is constructed by connecting the recognizing probe to a F0F1-ATPase molecular motor by means of an epsilon subunit antibody-biotin-avidin-5' end biotinylated vibrio parahemolyticus aptamer system. The lowest detection limit to the vibrio parahemolyticus by the method can reach 7cfu/mL, and the method can be applied to detecting vibrio parahemolyticus in shrimp meat samples, and is accurate and reliable in result.

The invention discloses a method for preparing an up-conversion aptamer test strip for rapid detection of ochratoxin A. The method comprises the following steps: modifying an up-conversion luminescentnano material, to be more specific, adding oleic acid and octadecene to an inorganic salt containing Yb<3+>, Y<3+> and Er<3+>, stirring, introducing an inert gas, and heating to form a uniform solution; dissolving ammonium fluoride and sodium hydroxide in methanol, adding the ammonium fluoride and sodium hydroxide methanol solution in the uniform solution, removing the methanol by heating and evaporation, introducing an inert gas to carry out reaction, adding a reaction product into a condensing reflux device, and heating to carry out reaction; mixing polyacrylic acid and ethanol to obtain apolyacrylic acid and ethanol mixed solution, dispersing the up-conversion luminescent nano material in a chloroform solution, adding the up-conversion luminescent nano material into the polyacrylic acid and ethanol mixed solution, and stirring for reaction to obtain a modified up-conversion luminescent nanomaterial; and preparing an up-conversion aptamer probe. The invention provides a new chromatographic test strip technology which can effectively improve the sensitivity of the test strip, reduce the production cost, and achieve the purpose of quantitative detection.

The invention discloses a primer set for detecting the COVID-19 virus. The primer set comprises an upstream primer and a downstream primer, and the primer set has relatively low sample requirements and strong amplification specificity, and greatly improves the sensitivity of clinical detection results. The invention also discloses a probe set for detecting the COVID-19 virus. The probe set comprises a first probe and a second probe, and the probes can bind to target single-stranded DNA by base complementary pairing respectively, and are captured by magnetic beads, so that the purpose of amplifying optical signals and performing quantitative detection is realized. The invention also discloses a kit for detecting the COVID-19 virus. The kit uses the primer set and the probe set, combines with the magnetic bead capture and optical signal amplification technology, has the characteristics of high sensitivity and high specificity, has simple operation, has low cost, and can be widely used inprimary medical institutions. The kit can be used to detect the COVID-19 virus, has stable and reliable detection results, has wide applicable scenarios, and has great market prospects.

The invention relates to microelectrode biosensing technology, and specifically discloses a microelectrode biosensor for in-situ living body detection of plant miRNA and an application thereof. In the present invention, after the gold nanoparticles are electrodeposited on the working electrode, the DNA probes capable of recognizing the target miRNA end-mercaptolated are incubated in the methylene blue MB solution to form a DNA-methylene blue complex, and then the complex is drip-coated to the deposition The working electrode surface of the gold nanoparticles was obtained, and the working electrode modified with the DNA probe was obtained. The microelectrode biosensor containing the working electrode is used to monitor living plants, and the change of peak current value of MB before and after hybridization is detected by electrochemical pulse voltammetry, so as to achieve the purpose of quantitative detection of microRNA without causing essential damage to the tested sample. The obtained data result can reflect the content change of the target miRNA in the plant in real time and dynamically, and the practical application is easy to operate and easy to grasp.

The invention discloses an LAMP (loop-mediated isothermal amplification) method for detecting the florfenicol resistance of bacteria through a series of research work of preparing materials, designing and synthesizing LAMP primers, extracting bacterial plasmid DNA (deoxyribonucleic acid), optimizing an LAMP reaction system and the like, and a corresponding LAMP primer group including an outer primer pair F3 and B3 and an inner primer pair FIP and BIP is designed according to sequences of florfenicol-resistant floR genes of the bacteria. For an unknown sample, the florfenicol resistance of a bacterial sample can be judged by detecting the value of the time when the turbidity value of floR gene amplification is 0.1 only. Specific and sensitive detection results prove that the florfenicol-resistant floR genes of the bacteria subjected to specific amplification by the method is high in sensitivity which is 100 times that of the florfenicol-resistant floR genes of the bacteria subjected to specific amplification by a conventional PCR method, the reaction can be monitored in real time, the copy number of the floR genes can be quantitatively detected, the detection results can be quickly and accurately obtained, and the convenience is brought to quick detection of the florfenicol resistance of the bacteria.

The invention discloses a method and a device for automatically detecting fiber content in textiles based on digital images, and belongs to the technical field of textile quality inspection. The method comprises the following steps: using the detection device to obtain fiber images for inspecting different focal planes of the textiles; compounding the different focal planes to obtain compounded clear fiber images; according to a shooting route, marking and counting specific fibers one by one through a counting method for the number of digital image fibers; and thus, realizing automatic detection for the fiber content in the textiles. The detection device comprises a computer, a three-dimensional controllable mobile platform and a high-power microscope, wherein the computer is provided withmodules such as a three-dimensional platform control and drive module, a digital image scanning and storage module and a digital image fiber root counting module. The fiber components are inspected by adopting a digital method, and the detection of fiber content in the textile is accurately and efficiently solved through automatic fiber counting.

The invention provides a method for detecting a 146S antigen in a foot-and-mouth disease vaccine based on a capillary electrophoresis method and application thereof. The method comprises the followingsteps of introducing a water-phase sample of the foot-and-mouth disease vaccine into a capillary tube by adopting pressure sample introduction, carrying out electrophoretic separation, detecting andrecording a characteristic peak of a 146S antigen in the water-phase sample, then integrating to obtain a peak area of the characteristic peak, and then obtaining the concentration of the 146S antigenaccording to a quantitative standard curve. The method provided by the invention can be used for quantitatively detecting multiple serotype 146S antigens at the same time, can be used for detecting monovalent, bivalent or trivalent foot-and-mouth disease vaccines, has the advantages of low sample size, high sensitivity, high detection speed and the like, has important significance for realizing rapid sampling inspection of market vaccines and improving the market detection efficiency, and meanwhile, has huge application value in the aspects of product research and development and quality supervision of multivalent foot-and-mouth disease vaccines.

The invention relates to a fluorescent detection reagent and a detection method for dichlorinated-1, 1'-dimethyl-4, 4'-dipyridyl, relating to a fluorescent detection reagent and method for a dipyridyl herbicide and aiming to solve the technical problems of difficulty in obtaining detection substances and complex operation in the traditional fluorescent detection method for paraquat. A fluorescent dye CXT is used as a fluorescent molecular probe in the reagent provided by the invention. The reagent is a liquid composed of water, a buffer liquid and the fluorescent dye CXT. Qualitative detection is realized through judging whether fluorescence quenching of CXT appears before and after the fluorescent detection reagent is added into a sample to be tested; and quantitative detection is realized by using a standard curve method. According to the invention, the fluorescent dye CXT is used as the fluorescent molecular probe, so that the fluorescent detection reagent can be used for detecting a DBPD herbicide in an actual environmental water sample such as lake water, sewage and the like.