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43results about How to "To achieve the purpose of quantitative detection" patented technology
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Parathion-methyl electrochemical biosensor based on sulfonic functionalized grapheme-chitosan
InactiveCN102706934AIncrease response currentAvoid interferenceMaterial electrochemical variablesElectrochemical responseStandard curve
A method adopted by an electrochemical biosensor based on sulfonic functionalized grapheme-chitosan to detect methyl parathion belongs to the technical field of electrochemical sensors and is used for detecting methyl parathion residual quantity in vegetables, fruits and products thereof. The basis of the invention is that methyl parathion can play an inhibition role on acetylcholinesterase, and the acetylcholinesterase can catalyze acetylthiocholine chloride to achieve electrochemical reaction of hydrosulfuryl acetylcholine. Sulfonic functionalized grapheme-chitosan is decorated on the surface of a bare glass carbon electrode, so that not only acetylcholinesterase is effectively fixed on the electrode, but also an electrochemical signal is amplified; and the methyl parathion residual quantity is related with electrochemical summit current, so that a standard curve for quantified detection of methyl parathion is built. The invention aims to provide the methyl parathion quantified detection method with high sensitivity and strong operability and develops the application of novel material graphene.
Owner:JIANGNAN UNIV +1
Gene expression method for simultaneously detecting 11 sports-related genes and 4 internal reference genes in human blood
InactiveCN101851674ATo achieve the purpose of quantitative detectionGood effectMicrobiological testing/measurementHuman bloodPolymerase chain reaction
The invention provides a gene expression method for simultaneously detecting 11 sports-related genes and 4 internal reference genes in human blood. The expression conditions of the 15 genes are detected simultaneously in the same reaction system by applying a multi-PCR, universal primer and nucleic acid hybridization method. The 11 sports-related genes are ciliary neurotrophic factor (CNTF) gene, beta2-adrenergic receptor (beta2-AR) gene, leptin receptor gene, transforming growth factor-beta1 (TGF-beta1) gene, mitochondrial uncoupling protein 2 gene, phospholipase C-gamma1 gene, tumor necrosis factor (TNF) gene, haptoglobin gene, RAD1 homologue gene, hypoxia inducible factor-1alpha (HIF-1alpha) gene and insulin-like growth factor-1 (IGF-1) of a human; and the 4 internal reference genes are histone deacetylase gene, glyceraldehyde-3-phosphate dehydrogenase gene, 18s-rRNA gene and beta-actin gene. Compared with the conventional real-time fluorescent quantitative polymerase chain reaction (PCR) detection method, the method of the invention has the advantages of high throughput, quickness, high automation degree, moderate cost, high accuracy and sensitivity and the like.
Owner:GENERAL HOSPITAL OF PLA
Method and system for detecting local impedance change of objects
InactiveCN102590348AAccurate quantitative identificationEasy to useAnalysing solids using sonic/ultrasonic/infrasonic wavesMeasurement of torque/twisting force while tighteningPattern recognitionObject based
The invention discloses a method and a system for detecting local impedance change of objects based on controlled knocking and a classifier. Classification is carried out by that an object has different local impedances and different response signals after being knocked and by the combination with a pattern recognition method. The method and the system not only can be used for detecting bolt screwing moment, but also can be used for detecting the damage status of composite material. The device adopted by the method is simpler, the method is simple and practical, the detection result is accurate, and multiclass classification can be realized.
Owner:TSINGHUA UNIV
Method and device for detecting bending strength of battery
ActiveCN103674727ATo achieve the purpose of quantitative detectionMaterial strength using steady bending forcesFlexural strengthElectrical and Electronics engineering
The invention discloses a method for detecting the bending strength of a battery. The method comprises the following steps: firstly, supporting a battery to be detected by two supporting points with the span of L; then applying a load P to a middle position between the two supporting points along the direction which is vertical to the surface of the battery to be detected; recording the size P1 of the load P when the battery to be detected has the deformation with the route a or recording the size P2 of the load P when the battery is damaged; and finally, substituting obtained data into a formula to calculate the value of the bending strength to realize the aim of quantitatively detecting the bending strength of the battery. With the adoption of the method disclosed by the invention, a specific numerical value for evaluating the bending strength of the battery can be obtained; a detection result is objective and the different detection results have the comparability. The invention further provides a device for detecting the bending strength of the battery.
Owner:HANGZHOU FUTURE POWER TECH +1
N-terminal brain natriuretic peptide precursor detection kit and detection method thereof
ActiveCN108613977AImprove stabilitySimultaneous detectionMaterial analysis by observing effect on chemical indicatorDisease diagnosisAntigenLatex particle
The invention discloses an N-terminal brain natriuretic peptide precursor detection kit and a detection method thereof. The kit comprises: reagents I: a phosphate buffer solution, a preservative, Tween 20 and polyethylene glycol; reagents II: a phosphate buffer solution, disodium hydrogen phosphate, sodium dihydrogen phosphate of 2 to 5 g / L, sodium chloride, a preservative and sensitized latex particles of a goat anti-human N-terminal-front B type natriuretic peptide antibody; and a calibration product: a phosphate buffer solution, bovine serum albumin, sodium chloride, a preservative and N-terminal-front B type natriuretic peptide antigen. According to the method, the test sensitivity is improved by applying a latex immunoturbidimetric method, the sensitized latex particles suitable for detecting an N-terminal front B type natriuretic peptide precursor are found, and the detection sensitivity is improved; large-batch samples can be detected quantitatively and simultaneously; and the kit comprises the calibration products and the quantitative detection aim is fulfilled.
Owner:SHAOXING SHENG KANG BIOLOGICAL TECH
Method for detecting treponema pallidum, quantum-dot labeled immunochromatography test paper and preparation method thereof
ActiveCN103529214AHigh detection sensitivityNarrow emission peakMaterial analysisCelluloseGlass fiber
The invention relates to a medical immunodetection method and particularly relates to a method for using quantum-dot labeled immunochromatography test paper to detect treponema pallidum by an immunological method. The quantum-dot labeled immunochromatography test paper is characterized in that a plastic board is stuck with a glass cellulose membrane A, a glass cellulose membrane B of a quantum-dot labeled treponema pallidum IgG monoclonal antibody, a nitrocellulose membrane and water absorbing paper from bottom to top in sequence, wherein one end of the nitrocellulose membrane is provided with treponema pallidum polyclonal antibody and rabbit antimouse second antibody so as to form a detecting band T and a quality control band C; the quantum-dot labeled treponema pallidum IgG monoclonal antibody is positioned at one end of the glass cellulose membrane and corresponds to the detecting band T and the quality control band C, and the quantum-dot labeled treponema pallidum IgG monoclonal antibody is positioned at one end of a sampling point. The method has the advantages that high specificity of immunoreactions and the fluorescence characteristic of quantum dots are combined, so that the detection sensitivity is about 1000 times higher than that of the current commonly-used colloidal gold detection method.
Owner:北京华卫骥生物医药有限公司
Gold labeled immunochromatographic strip assay semiquantitative detection test paper for detecting dienestrol
The invention provides gold labeled immunochromatographic strip assay semiquantitative detection test paper for detecting dienestrol. The test paper is characterized in that a sample absorption pad, a colloid gold label pad, a chromatography pad and a water absorption pad are pasted on the back lining of the test paper; and a detection reaction pad is covered by dienestrol antigen serving as a detection line and second antibody serving as a quality control line at the same time. The semiquantitative detection test paper has the advantages that the test paper has strong specificity and can realize semiquantitative detection; the test paper can be used at a temperature of between 4 and 40 DEG C and can obtain a result after 5 minutes; and the test paper is suitable for quickly detecting dienestrol drugs in animal-derived foodstuff samples by sanitation departments, quality inspection departments, customs and livestock farms and foodstuff enterprises.
Owner:INST OF QUALITY STANDARD & TESTING TECH FOR AGRO PROD OF CAAS
Preparation method and application of immunosensor based on titanium dioxide-doped graphene-loaded trepan-shaped gold palladium core-shell nanoparticles
InactiveCN108896638AIncrease the areaLarge specific surface areaMaterial analysis by electric/magnetic meansBiological testingDoped grapheneCore shell nanoparticles
The invention belongs to the technical fields of novel functional materials, immunoassay and biosensing and provides a preparation method and application of an immunosensor based on titanium dioxide-doped graphene-loaded trepan-shaped gold palladium core-shell nanoparticles. A marker-free electrochemical immunosensor is constructed by taking the titanium dioxide-doped graphene-loaded trepan-shapedgold palladium core-shell nanoparticles as a signal amplifying platform, so that the quantitative determination of ovarian cancer markers is realized, and the immunosensor has the advantages of strong specificity, high sensitivity, low detection limit and the like. The specificity has important scientific significances and application values in the detection of ovary diseases.
Owner:SHANDONG UNIV OF TECH
Method for detecting Vibrio parahemolyticus based on aptamer identification-molecular motor biosensing
InactiveCN108169483AImprove stabilityReduce manufacturing costFluorescence/phosphorescenceFluorescenceBiotin-binding proteins
The invention provides a method for detecting Vibrio parahemolyticus based on aptamer identification-molecular motor biosensing. According to the method, the interior of a chromatophore is labeled with a pH-sensitive fluorescent dye F1300 so that a signal probe is obtained, an aptamer specifically recognizing Vibrio parahemolyticus is used as a recognition probe, and an epsilon subunit antibody-biotin-avidin-end 5' biotinylated Vibrio parahemolyticus aptamer system connects the recognition probe and an F0F1-ATPase molecule motor so that the F0F1-ATPase molecule motor aptamer sensing system isconstructed. The method has the lowest detection limit of 15cfu / mL to Vibrio parahemolyticus, can be used for detection of Vibrio parahemolyticus in a salmon sample and can produce an accurate and reliable result.
Owner:JIANGNAN UNIV
Method for detecting poliovirus, quantum dot-labeled immunochromatographic test paper and preparation method thereof
InactiveCN103529217AHigh detection sensitivityNarrow emission peakBiological material analysisCelluloseGlass fiber
The invention relates to a medimmune inspection method, and particularly relates to quantum dot-labeled immunochromatographic test paper, and a method for detecting poliovirus by adopting an immunological method. According to the quantum dot-labeled immunochromatographic test paper, a glass cellulose membrane A, a quantum dot-labeled poliovirus IgG monoclonal antibody glass cellulose membrane B, a cellulose nitrate membrane and absorbent paper are sequentially are bonded on a plastic board from bottom to top, wherein a poliovirus polyclonal antibody and a rabbit-anti-mouse secondary antibody are on one end of the cellulose nitrate membrane so as to form an inspection strip T and a quality control strip C; a quantum dot-labeled poliovirus IgG monoclonal antibody is located at one end of the glass cellulose membrane B and corresponds to the inspection strip T and the quality control strip C, and the quantum dot-labeled poliovirus IgG monoclonal antibody is located at one end of a sample feeding point. The inspection sensitivity of the method is higher than of the currently used method by about 1000 times.
Owner:BEIJING HUAWEI BRAVOBIO
Biosensor and method of detecting vibrio parahemolyticus thereby
InactiveCN108120703AImprove stabilityReduce manufacturing costFluorescence/phosphorescenceFluorescenceBiology
The invention provides a method of detecting vibrio parahemolyticus based on an aptamer recognizing quantum dot labelled molecular motor biosensor. The aptamer of vibrio parahemolyticus is specifically recognized as a recognizing probe by labeling a pH sensitive quantum dot fluorescent nanomaterial to the surface of a color carrier. An F0F1 ATPas molecular motor aptamer sensing system is constructed by connecting the recognizing probe to a F0F1-ATPase molecular motor by means of an epsilon subunit antibody-biotin-avidin-5' end biotinylated vibrio parahemolyticus aptamer system. The lowest detection limit to the vibrio parahemolyticus by the method can reach 7cfu / mL, and the method can be applied to detecting vibrio parahemolyticus in shrimp meat samples, and is accurate and reliable in result.
Owner:JIANGNAN UNIV
Method for preparing up-conversion aptamer test strip for rapid detection of ochratoxin A
The invention discloses a method for preparing an up-conversion aptamer test strip for rapid detection of ochratoxin A. The method comprises the following steps: modifying an up-conversion luminescentnano material, to be more specific, adding oleic acid and octadecene to an inorganic salt containing Yb<3+>, Y<3+> and Er<3+>, stirring, introducing an inert gas, and heating to form a uniform solution; dissolving ammonium fluoride and sodium hydroxide in methanol, adding the ammonium fluoride and sodium hydroxide methanol solution in the uniform solution, removing the methanol by heating and evaporation, introducing an inert gas to carry out reaction, adding a reaction product into a condensing reflux device, and heating to carry out reaction; mixing polyacrylic acid and ethanol to obtain apolyacrylic acid and ethanol mixed solution, dispersing the up-conversion luminescent nano material in a chloroform solution, adding the up-conversion luminescent nano material into the polyacrylic acid and ethanol mixed solution, and stirring for reaction to obtain a modified up-conversion luminescent nanomaterial; and preparing an up-conversion aptamer probe. The invention provides a new chromatographic test strip technology which can effectively improve the sensitivity of the test strip, reduce the production cost, and achieve the purpose of quantitative detection.
Owner:JIANGNAN UNIV
Primer set, probe set and kit for detecting COVID-19 virus, and application of kit
PendingCN111647685AHigh sensitivityHigh specificityMicrobiological testing/measurementAgainst vector-borne diseasesBioinformaticsSingle strand
The invention discloses a primer set for detecting the COVID-19 virus. The primer set comprises an upstream primer and a downstream primer, and the primer set has relatively low sample requirements and strong amplification specificity, and greatly improves the sensitivity of clinical detection results. The invention also discloses a probe set for detecting the COVID-19 virus. The probe set comprises a first probe and a second probe, and the probes can bind to target single-stranded DNA by base complementary pairing respectively, and are captured by magnetic beads, so that the purpose of amplifying optical signals and performing quantitative detection is realized. The invention also discloses a kit for detecting the COVID-19 virus. The kit uses the primer set and the probe set, combines with the magnetic bead capture and optical signal amplification technology, has the characteristics of high sensitivity and high specificity, has simple operation, has low cost, and can be widely used inprimary medical institutions. The kit can be used to detect the COVID-19 virus, has stable and reliable detection results, has wide applicable scenarios, and has great market prospects.
Owner:广州中科抗体生物技术有限公司
A method and device for detecting battery bending strength
ActiveCN103674727BTo achieve the purpose of quantitative detectionMaterial strength using steady bending forcesFlexural strengthUltimate tensile strength
Owner:HANGZHOU FUTURE POWER TECH +1
Quantitative detector for Muscae volitantes
InactiveCN104545784ATo achieve the purpose of quantitative detectionSimple structureEye diagnosticsCamera lensFlexible endoscope
The invention discloses a quantitative detector for muscae volitantes. The quantitative detector is a metal square box device forming an all-closed visual space. Milk white PC plastic is arranged on the inner wall of the square box device, and a lower jaw carrier is mounted at the lower end of the square box device and is connected to a table top through a left stand column and a right stand column. A left eye endoscopy lens and a right eye endoscopy lens are mounted on the outer side of the front end of the square box device. A movable shielding piece is mounted on each of the left eye endoscopy lens and the right eye endoscopy lens. Four inner visual space supplement light sources are mounted at four inner corners of the front end of the square box device. A liquid crystal display screen is mounted at the rear end of the square box device and is connected with a computer of a doctor. The liquid crystal display screen and a screen of the computer of the doctor can sequentially display eight muscae volitantes quantitative detection template pages synchronously. The quantitative detector is simple in structure, convenient to operate and capable of detecting muscae volitantes of patients quantitatively; the muscae volitantes of the patients can be discovered by the doctor in an outpatient department timely, and the objectives of early discovery and early treatment are achieved; the doctor in the outpatient department can acquire accurate and clear quantitative cognition of muscae volitantes symptoms of the patients, and make a quantitative detection report for the muscae volitantes.
Owner:孟庆娜
Microelectrode biosensor of in-situ vivo detection plant miRNA and application of microelectrode biosensor
InactiveCN106248771ASimple and efficient operationEasy to masterMaterial electrochemical variablesMicroelectrodeBiology
The invention relates to microelectrode biosensing technology, and specifically discloses a microelectrode biosensor for in-situ living body detection of plant miRNA and an application thereof. In the present invention, after the gold nanoparticles are electrodeposited on the working electrode, the DNA probes capable of recognizing the target miRNA end-mercaptolated are incubated in the methylene blue MB solution to form a DNA-methylene blue complex, and then the complex is drip-coated to the deposition The working electrode surface of the gold nanoparticles was obtained, and the working electrode modified with the DNA probe was obtained. The microelectrode biosensor containing the working electrode is used to monitor living plants, and the change of peak current value of MB before and after hybridization is detected by electrochemical pulse voltammetry, so as to achieve the purpose of quantitative detection of microRNA without causing essential damage to the tested sample. The obtained data result can reflect the content change of the target miRNA in the plant in real time and dynamically, and the practical application is easy to operate and easy to grasp.
Owner:BEIJING RES CENT FOR INFORMATION TECH & AGRI
LAMP (loop-mediated isothermal amplification) method for detecting florfenicol resistance of bacteria
InactiveCN103937883AEasy way to judge the resultAvoid pollutionMicrobiological testing/measurementDNA/RNA fragmentationFlorTurbidity
The invention discloses an LAMP (loop-mediated isothermal amplification) method for detecting the florfenicol resistance of bacteria through a series of research work of preparing materials, designing and synthesizing LAMP primers, extracting bacterial plasmid DNA (deoxyribonucleic acid), optimizing an LAMP reaction system and the like, and a corresponding LAMP primer group including an outer primer pair F3 and B3 and an inner primer pair FIP and BIP is designed according to sequences of florfenicol-resistant floR genes of the bacteria. For an unknown sample, the florfenicol resistance of a bacterial sample can be judged by detecting the value of the time when the turbidity value of floR gene amplification is 0.1 only. Specific and sensitive detection results prove that the florfenicol-resistant floR genes of the bacteria subjected to specific amplification by the method is high in sensitivity which is 100 times that of the florfenicol-resistant floR genes of the bacteria subjected to specific amplification by a conventional PCR method, the reaction can be monitored in real time, the copy number of the floR genes can be quantitatively detected, the detection results can be quickly and accurately obtained, and the convenience is brought to quick detection of the florfenicol resistance of the bacteria.
Owner:GUANGXI VETERINARY RES INST
Method and device for automatically detecting fiber content in textiles based on digital images
ActiveCN107860933ARealize automatic countingGuaranteed accuracyMaterial analysisComputer visionDigital image
The invention discloses a method and a device for automatically detecting fiber content in textiles based on digital images, and belongs to the technical field of textile quality inspection. The method comprises the following steps: using the detection device to obtain fiber images for inspecting different focal planes of the textiles; compounding the different focal planes to obtain compounded clear fiber images; according to a shooting route, marking and counting specific fibers one by one through a counting method for the number of digital image fibers; and thus, realizing automatic detection for the fiber content in the textiles. The detection device comprises a computer, a three-dimensional controllable mobile platform and a high-power microscope, wherein the computer is provided withmodules such as a three-dimensional platform control and drive module, a digital image scanning and storage module and a digital image fiber root counting module. The fiber components are inspected by adopting a digital method, and the detection of fiber content in the textile is accurately and efficiently solved through automatic fiber counting.
Owner:信融源大数据科技(北京)有限公司 +1
Method for detecting 146S antigen in foot-and-mouth disease vaccine based on capillary electrophoresis method and application thereof
ActiveCN112034028AAvoid structureAvoid lostMaterial analysis by electric/magnetic meansAntigenDisease
The invention provides a method for detecting a 146S antigen in a foot-and-mouth disease vaccine based on a capillary electrophoresis method and application thereof. The method comprises the followingsteps of introducing a water-phase sample of the foot-and-mouth disease vaccine into a capillary tube by adopting pressure sample introduction, carrying out electrophoretic separation, detecting andrecording a characteristic peak of a 146S antigen in the water-phase sample, then integrating to obtain a peak area of the characteristic peak, and then obtaining the concentration of the 146S antigenaccording to a quantitative standard curve. The method provided by the invention can be used for quantitatively detecting multiple serotype 146S antigens at the same time, can be used for detecting monovalent, bivalent or trivalent foot-and-mouth disease vaccines, has the advantages of low sample size, high sensitivity, high detection speed and the like, has important significance for realizing rapid sampling inspection of market vaccines and improving the market detection efficiency, and meanwhile, has huge application value in the aspects of product research and development and quality supervision of multivalent foot-and-mouth disease vaccines.
Owner:INST OF PROCESS ENG CHINESE ACAD OF SCI
Electrochemical biosensor for detecting bisphenol-A, and preparation method and application thereof
InactiveCN103278541BIncrease response currentTo achieve the purpose of quantitative detectionMaterial electrochemical variablesElectrochemical biosensorSurface modification
Owner:NINGBO UNIV
Fluorescent detection reagent and detection method for dichlorinated-1, 1'-dimethyl-4, 4'-dipyridyl
InactiveCN103952149AHigh selectivityIncreased sensitivityFluorescence/phosphorescenceLuminescent compositionsFluorescenceQuenching
The invention relates to a fluorescent detection reagent and a detection method for dichlorinated-1, 1'-dimethyl-4, 4'-dipyridyl, relating to a fluorescent detection reagent and method for a dipyridyl herbicide and aiming to solve the technical problems of difficulty in obtaining detection substances and complex operation in the traditional fluorescent detection method for paraquat. A fluorescent dye CXT is used as a fluorescent molecular probe in the reagent provided by the invention. The reagent is a liquid composed of water, a buffer liquid and the fluorescent dye CXT. Qualitative detection is realized through judging whether fluorescence quenching of CXT appears before and after the fluorescent detection reagent is added into a sample to be tested; and quantitative detection is realized by using a standard curve method. According to the invention, the fluorescent dye CXT is used as the fluorescent molecular probe, so that the fluorescent detection reagent can be used for detecting a DBPD herbicide in an actual environmental water sample such as lake water, sewage and the like.
Owner:QIQIHAR UNIVERSITY
Preparation method for quantum dot labelled immunochromatographic test strip
ActiveCN103543268BHigh detection sensitivityNarrow emission peakBiological material analysisGlass fiberCellulose
The invention relates to a medical immunodetection method and in particular relates to a method for detecting a Japanese encephalitis virus (JEV) by an immunological method by using a quantum dot labelled immunochromatographic test strip. The quantum dot labelled immunochromatographic test strip is characterized in that a glass cellulose membrane A, a glass cellulose membrane B of a quantum dot labelled JEV IgG (immunoglobulin G) monoclonal antibody, a nitrocellulose membrane and absorbent paper are stuck to a plastic board from bottom to top in sequence, wherein one end of the nitrocellulose membrane has a JEV polyclonal antibody and a rabbit anti-mouse second antibody, thereby forming a detection zone T and a quality control zone C; the quantum dot labelled JEV IgG monoclonal antibody is arranged at the other end of the glass cellulose membrane B, corresponds to the detection zone T and the quality control zone C and is arranged at one end of a sampling point. The detection sensitivity of the method is about 1000 times higher than that of the detection method frequently used at present.
Owner:北京华卫骥生物医药有限公司
A composite film sensor and method for detecting vibrio parahaemolyticus
ActiveCN108226135BTo achieve the purpose of quantitative detectionHigh sensitivityRaman scatteringAptamerShrimp
The invention provides a method for detecting vibrio parahemolyticus by an AU-PDMS composite membrane on the basis of aptamer identification. A specific recognition vibrio parahemolyticus aptamer is fixed on the surface of nanogold, a Raman signal module is modified as a signal probe, the specific recognition vibrio parahemolyticus is fixed on an APT-AU-PDMS composite membrane formed by a charge effect to serve as a detection substrate, and a 'sandwich' structure detection sensor is established. The lowest detection limit of the method for the vibrio parahemolyticus can be 12cfu / mL, in addition, the method can be applied to the detection of the vibrio parahemolyticus in shrimp samples, and a result is accurate and reliable.
Owner:JIANGNAN UNIV
A detection kit for N-terminal brain natriuretic peptide precursor
ActiveCN108613977BImprove stabilitySimultaneous detectionMaterial analysis by observing effect on chemical indicatorDisease diagnosisAntigenPolyethylene glycol
The invention discloses a N-terminal brain natriuretic peptide precursor detection kit and a detection method thereof; the kit includes: reagent one: phosphate buffer, preservative, Tween 20, polyethylene glycol 6000; reagent two: Phosphate buffer, disodium hydrogen phosphate, 2-5g / L sodium dihydrogen phosphate, sodium chloride, preservatives, sensitized latex particles of goat anti-human N-terminal-pre-B type natriuretic peptide antibody; calibrator: phosphoric acid Saline buffer solution, bovine serum albumin, sodium chloride, preservatives, N-terminal-pro-B-type natriuretic peptide antigen; method The latex immunoturbidimetric method was used to improve the sensitivity of the test, and to find a suitable detection method for the N-terminal pro-BNP Sensitized latex particles to improve detection sensitivity; through different automatic biochemical analyzers with different reagent-sample ratios, a large number of samples can be quantitatively detected at the same time; the kit contains calibrators to achieve the purpose of quantitative detection.
Owner:SHAOXING SHENG KANG BIOLOGICAL TECH
Preparation method for quantum-dot labeled immunochromatography test paper
ActiveCN103529216BHigh detection sensitivityNarrow emission peakBiological material analysisGlass fiberCellulose
Owner:北京华卫骥生物医药有限公司
Gas-assisted detection device
The invention relates to the technical field of safety protection and discloses a gas auxiliary detection device, which includes a bracket and a box body. The box body is fixed on the bracket. The box body is provided with an inner cavity and an air inlet rod; the air inlet rod is equipped with an annular cavity. There is an air inlet on the cavity, a high-pressure air box is fixed on the bracket, baffles and air bags are provided on both sides of the inner cavity, a first sprocket is fixed on the rotating shaft, and an infrared light is installed on the bottom of the baffle; There is an opening and a detection box. The detection box is provided with a first glass plate. There is a photoresistor in the detection box. The photoresistor is electrically connected to an indicator light. The bracket is provided with an internal meshing ratchet structure. The internal meshing ratchet mechanism includes an outer ratchet and an active gear. The pawl, the non-return pawl and the driving wheel. A second sprocket is fixed inside the driving wheel. A turntable is fixed on the outer edge of the outer ratchet. A second glass plate is provided on the turntable, and a transparent tape is provided on the second glass plate. The sticky side of the clear tape faces the lumen. The invention has a simple structure and can detect air without filtering particles.
Owner:重庆博创知识产权运营有限公司
Preparation method for quantum-dot-marked immunochromatographic test paper
ActiveCN103543269BHigh detection sensitivityNarrow emission peakMaterial analysisCelluloseGlass fiber
Owner:CHINA BEIJING BEIDA JUBANG SCI & TECH CO LTD
Method for detecting rubella virus, quantum dot-labeled immunochromatographic test paper and preparation method thereof
ActiveCN103529211BHigh detection sensitivityNarrow emission peakBiological material analysisCelluloseGlass fiber
The invention relates to a medimmune inspection method, and particularly relates to quantum dot-labeled immunochromatographic test paper, and a method for detecting rubella virus by adopting an immunological method. According to the quantum dot-labeled immunochromatographic test paper, a glass cellulose membrane A, a quantum dot-labeled rubella virus IgG monoclonal antibody glass cellulose membrane B, a cellulose nitrate membrane and absorbent paper are sequentially bonded on a plastic board from bottom to top, wherein a rubella virus polyclonal antibody and a rabbit-anti-mouse secondary antibody are arranged on one end of the cellulose nitrate membrane so as to form an inspection strip T and a quality control strip C; a quantum dot-labeled rubella virus IgG monoclonal antibody is located at the other end of the glass cellulose membrane B and corresponds to the inspection strip T and the quality control strip C, and the quantum dot-labeled rubella virus IgG monoclonal antibody is located at one end of a sample feeding point. The inspection sensitivity of the method is higher than of the currently used method by about 1000 times.
Owner:魏县聚邦新材料科技有限公司
Preparation method and application of an immunosensor based on titanium dioxide-doped graphene-loaded sea cucumber-shaped gold-palladium core-shell nanoparticles
InactiveCN108896638BIncrease the areaLarge specific surface areaMaterial analysis by electric/magnetic meansBiological testingDiseaseImmune profiling
Owner:SHANDONG UNIV OF TECH
LAMP method for detecting bacterial resistance to florfenicol
InactiveCN103937883BHigh sensitivityEasy way to judge the resultMicrobiological testing/measurementDNA/RNA fragmentationFlorTurbidity
The invention discloses an LAMP (loop-mediated isothermal amplification) method for detecting the florfenicol resistance of bacteria through a series of research work of preparing materials, designing and synthesizing LAMP primers, extracting bacterial plasmid DNA (deoxyribonucleic acid), optimizing an LAMP reaction system and the like, and a corresponding LAMP primer group including an outer primer pair F3 and B3 and an inner primer pair FIP and BIP is designed according to sequences of florfenicol-resistant floR genes of the bacteria. For an unknown sample, the florfenicol resistance of a bacterial sample can be judged by detecting the value of the time when the turbidity value of floR gene amplification is 0.1 only. Specific and sensitive detection results prove that the florfenicol-resistant floR genes of the bacteria subjected to specific amplification by the method is high in sensitivity which is 100 times that of the florfenicol-resistant floR genes of the bacteria subjected to specific amplification by a conventional PCR method, the reaction can be monitored in real time, the copy number of the floR genes can be quantitatively detected, the detection results can be quickly and accurately obtained, and the convenience is brought to quick detection of the florfenicol resistance of the bacteria.
Owner:GUANGXI VETERINARY RES INST
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