Parathion-methyl electrochemical biosensor based on sulfonic functionalized grapheme-chitosan

A methyl parathion electric and biosensor technology, applied in the field of ultra-sensitivity and easy operability, can solve the problems of expensive, high cost, bulky and bulky instruments, and achieve the advantages of improving accuracy and sensitivity, preventing interference, and improving response current. Effect

Inactive Publication Date: 2012-10-03
JIANGNAN UNIV +1
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  • Abstract
  • Description
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Problems solved by technology

Although the traditional methyl parathion detection methods, chromatography and color-mass spectrometry, are sensitive and accurate, they require cumbersome pretreatment of samples, a

Method used

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  • Parathion-methyl electrochemical biosensor based on sulfonic functionalized grapheme-chitosan
  • Parathion-methyl electrochemical biosensor based on sulfonic functionalized grapheme-chitosan
  • Parathion-methyl electrochemical biosensor based on sulfonic functionalized grapheme-chitosan

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Embodiment 1

[0016] The prepared electrochemical enzyme sensor was placed in the methyl parathion standard solution for reaction, and the concentration of the standard solution was as follows: 0ng / mL, 0.01ng / mL, 0.05ng / mL, 0.1ng / mL, 0.5ng / mL mL, 1ng / mL, 5ng / mL, 10ng / mL, 50ng / mL, 100ng / mL, the working voltage is 0.2V~1.0V, the scan rate is 100mV / s, and the working buffer system is NaH 2 PO 4 -Na 2 HPO 4 (pH=7.0), each concentration was inhibited for 16 min respectively; after the electrode was taken out, the surface of the electrode was cleaned with ultrapure water to remove residual pesticides, and then the electrode was placed in a reaction pool with a concentration of 5 mM acetylcholine chloride to react for 2 min. Formula A%=(I 0 -I p ) / I 0 ×100% (A% represents enzyme inhibition rate, 1 0 and I p Represent not being inhibited by pesticides and the peak current value of the reaction after being inhibited by pesticides) Calculate the inhibition rate of immobilized enzyme, and estab...

Embodiment 2

[0018] Tear off the cabbage leaves and spread out, spray the pesticide evenly on the leaves, then chop and mix well. Weigh 10.00g of the mixed sample, place it in a 250mL Erlenmeyer flask with stopper, add 30g of anhydrous sodium sulfate for dehydration, and shake vigorously; then add 0.25g of activated carbon for decolorization; then add 70mL of dichloromethane, and shake Shake for 30 min, and filter the mixture with filter paper. Measure 35mL of the filtrate, volatilize naturally in a fume hood at room temperature until nearly dry, grind and wash the residue 3 times with a small amount of dichloromethane, transfer to a 5mL graduated centrifuge tube, and dilute to 2.0mL for later use. The selected working voltage is 0.2V~1.0V, the scanning speed is 100mV / s, and the working buffer system is NaH 2 PO 4 -Na 2 HPO 4 (pH=7.0), the prepared enzyme electrode was placed in the extract solution for 16min, after taking out the electrode, the surface of the electrode was cleaned wit...

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Abstract

A method adopted by an electrochemical biosensor based on sulfonic functionalized grapheme-chitosan to detect methyl parathion belongs to the technical field of electrochemical sensors and is used for detecting methyl parathion residual quantity in vegetables, fruits and products thereof. The basis of the invention is that methyl parathion can play an inhibition role on acetylcholinesterase, and the acetylcholinesterase can catalyze acetylthiocholine chloride to achieve electrochemical reaction of hydrosulfuryl acetylcholine. Sulfonic functionalized grapheme-chitosan is decorated on the surface of a bare glass carbon electrode, so that not only acetylcholinesterase is effectively fixed on the electrode, but also an electrochemical signal is amplified; and the methyl parathion residual quantity is related with electrochemical summit current, so that a standard curve for quantified detection of methyl parathion is built. The invention aims to provide the methyl parathion quantified detection method with high sensitivity and strong operability and develops the application of novel material graphene.

Description

technical field [0001] The invention relates to the detection of methyl parathion by using an electrochemical sensor, and is characterized by the application of sulfonic acid functionalized graphene, and the ultra-sensitive and easy-to-operate detection method. Background technique [0002] Methyl parathion is a highly toxic organophosphate insecticide with a broad spectrum. efficient. It has the advantages of small amount, multiple modes of action, convenient use, and short half-life. It has been widely used in agricultural production and played an important role in the control of various pests on various crops. However, its residues not only cause serious pollution and harm to the ecological environment, but also can accumulate in the human body through the food chain, causing various diseases and endangering human health. Therefore, it has been banned in many countries, but highly toxic compounds such as methyl parathion are still important pollutants for routine detect...

Claims

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Application Information

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IPC IPC(8): G01N27/26G01N27/327
Inventor 王周平张伟龙贾飞何良兴魏云潇金旭忠
Owner JIANGNAN UNIV
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