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Preparation method and application of immunosensor based on titanium dioxide-doped graphene-loaded trepan-shaped gold palladium core-shell nanoparticles

An immune sensor and aminated graphene technology, applied in the direction of instruments, scientific instruments, material inspection products, etc., can solve the problems of missed diagnosis and misdiagnosis, atypical early symptoms, difficult early diagnosis, etc., achieve rapid detection, increase specific surface area, The effect of improving sensitivity

Inactive Publication Date: 2018-11-27
SHANDONG UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the atypical early symptoms of ovarian cancer clinical symptoms, it is very easy to cause missed diagnosis and misdiagnosis, which is a big problem in early diagnosis

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Embodiment 1. A kind of preparation method based on the immunosensor of titanium dioxide-doped graphene-loaded sea cucumber-shaped gold-palladium core-shell nanoparticles, the operation steps are as follows:

[0039] (1) The glassy carbon electrode with a diameter of 4.0 mm was polished into a mirror surface with 1.0 µm, 0.3 µm and 0.05 µm alumina polishing powder in sequence, and cleaned ultrasonically in absolute ethanol;

[0040] (2) Take 6.0 μL, 1.0 mg / mL of titanium dioxide-doped graphene-loaded sea cucumber-like gold-palladium core-shell nanoparticles dispersion and apply it to the electrode surface, dry it at room temperature, rinse the electrode surface with ultrapure water, and dry it;

[0041] (3) Continue to drop 6.0 µL, 10.0 µg / mL human epididymis protein 4 antibody onto the surface of the electrode, and let it dry in a refrigerator at 4 °C;

[0042] (4) Continue to add 3.0 µL, 1.0 wt% bovine serum albumin solution dropwise to the electrode surface to seal t...

Embodiment 2

[0044] Embodiment 2. A kind of preparation method based on the immunosensor of titanium dioxide-doped graphene-loaded sea cucumber-shaped gold-palladium core-shell nanoparticles, the operation steps are as follows:

[0045] (1) The glassy carbon electrode with a diameter of 4.0 mm was polished into a mirror surface with 1.0 µm, 0.3 µm and 0.05 µm alumina polishing powder in sequence, and cleaned ultrasonically in absolute ethanol;

[0046] (2) Take 6.0 μL, 2.0 mg / mL of titanium dioxide-doped graphene-loaded sea cucumber-shaped gold-palladium core-shell nanoparticles dispersion and apply it to the surface of the electrode, dry it at room temperature, rinse the surface of the electrode with ultrapure water, and dry it;

[0047] (3) Continue to drop 6.0 µL, 10.0 µg / mL human epididymis protein 4 antibody onto the surface of the electrode, and let it dry in a refrigerator at 4 °C;

[0048] (4) Continue to add 3.0 µL, 1.0 wt% bovine serum albumin solution dropwise to the electrode s...

Embodiment 3

[0050] Embodiment 3. A kind of preparation method based on the immunosensor of titanium dioxide-doped graphene-loaded sea cucumber-shaped gold-palladium core-shell nanoparticles, the operation steps are as follows:

[0051] (1) The glassy carbon electrode with a diameter of 4.0 mm was polished into a mirror surface with 1.0 µm, 0.3 µm and 0.05 µm alumina polishing powder in sequence, and cleaned ultrasonically in absolute ethanol;

[0052] (2) Take 6.0 μL, 3.0 mg / mL of titanium dioxide-doped graphene-loaded sea cucumber-shaped gold-palladium core-shell nanoparticles dispersion and apply it to the surface of the electrode, dry it at room temperature, rinse the surface of the electrode with ultrapure water, and dry it;

[0053] (3) Continue to drop 6.0 µL, 10.0 µg / mL human epididymis protein 4 antibody onto the surface of the electrode, and let it dry in a refrigerator at 4 °C;

[0054] (4) Continue to add 3.0 µL, 1.0 wt% bovine serum albumin solution dropwise to the electrode s...

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Abstract

The invention belongs to the technical fields of novel functional materials, immunoassay and biosensing and provides a preparation method and application of an immunosensor based on titanium dioxide-doped graphene-loaded trepan-shaped gold palladium core-shell nanoparticles. A marker-free electrochemical immunosensor is constructed by taking the titanium dioxide-doped graphene-loaded trepan-shapedgold palladium core-shell nanoparticles as a signal amplifying platform, so that the quantitative determination of ovarian cancer markers is realized, and the immunosensor has the advantages of strong specificity, high sensitivity, low detection limit and the like. The specificity has important scientific significances and application values in the detection of ovary diseases.

Description

technical field [0001] The invention belongs to the technical fields of novel nanomaterials, immune analysis and biosensing, and provides a method for preparing an immunosensor based on titania-doped graphene-loaded sea cucumber-shaped gold-palladium core-shell nanoparticles, and an electrochemical sensor constructed by the method. Application of immunosensor in detection of human epididymis protein 4 antigen. Background technique [0002] Ovarian cancer is a common malignant tumor in gynecology, and its mortality rate ranks first in gynecological malignant tumors. Therefore, timely screening and detection in the early stage of ovarian cancer is an important way to reduce mortality. However, due to the atypical early symptoms of ovarian cancer clinical symptoms, it is very easy to cause missed diagnosis and misdiagnosis, which is a big problem in early diagnosis. Therefore, it is very important to develop rapid, simple and sensitive methods for detecting ovarian cancer mar...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/327G01N33/68G01N33/574
CPCG01N27/308G01N27/3275G01N33/57449G01N33/57484G01N33/689
Inventor 刘青颜芹刘会谭召灵董慧董云会
Owner SHANDONG UNIV OF TECH
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