20results about How to "Improve electron transfer efficiency" patented technology

The invention relates to the technical field of wastewater treatment, in particular to a hydrolytic acidification wastewater treatment process for strengthening extracellular electron transfer, which comprises the following steps: S1, constructing a hydrolytic acidification reactor, taking carbon fiber suspension filler as an anode, taking a steel wire mesh frame as a cathode, connecting the carbon fiber suspension filler in series by a stainless steel wire rope, and taking the stainless steel wire rope as a cathode; the carbon fiber suspension filler is connected with the steel wire mesh frame through an electrochemical workstation; s2, inoculating hydrolytic acidification bacteria into a reactor, controlling culture conditions, and domesticating microorganisms in a micro-current environment until a biological membrane is formed on the carbon fiber suspension filler; s3, inputting wastewater to be treated into the reactor, controlling treatment conditions, monitoring an effluent result, and discharging after the effluent result reaches the standard. Compared with traditional interspecific electron transfer, the wastewater treatment method has higher efficiency and speed, the oxygenolysis process of organic matter is accelerated, and the overall efficiency of wastewater treatment is improved.

The invention discloses an acrylamide monomer solution production method which comprises the following steps: adding acrylonitrile, water and immobilized nitrile hydratase into a reaction kettle for hydration reaction to prepare an acrylamide monomer; the reaction kettle comprises a kettle body, a kettle cover is arranged at the upper end of the kettle body, a discharging pipe is arranged at the bottom of the kettle body, a first feeding pipe, a second feeding pipe, a third feeding pipe and a stirring motor are arranged on the kettle cover, a stirring shaft is arranged on an output shaft of the stirring motor, and stirring paddles are distributed on the stirring shaft; an annular distributor is further arranged in the kettle body and comprises a distribution cavity, the upper end of the distribution cavity is connected with an adjusting cavity, a spraying head is arranged in the adjusting cavity and provided with spraying holes communicated with the distribution cavity, the distribution cavity is communicated with the bottom of the kettle body through a circulating pipe, and a circulating pump is arranged on the circulating pipe. According to the method disclosed by the invention, acrylonitrile is hydrolyzed by using the immobilized nitrile hydratase with high stability, so that the problems of relatively poor enzyme stability and relatively high production cost in preparation of acrylamide by an existing biological catalysis method are solved.

The invention relates to the technical field of water pollution control, in particular to a method for rapidly starting a dye-organic wastewater anaerobic co-metabolism system based on S-nZVI, which comprises the following steps: S1, preparing S-nZVI, and S2, optimizing an anaerobic treatment method of dye-organic wastewater by S-nZVI. According to the method, the sulfur-doped nano-iron is applied to a dye-organic wastewater anaerobic co-metabolism system for the first time, the dispersity of the nano-iron is improved through sulfur doping, the hydrogen evolution reaction is inhibited through sulfur doping, and reduction damage of cell membranes is avoided; meanwhile, the formation of FeS endows the nano-iron with stronger conductivity, electron transfer pathways of microorganisms are enriched, the electron transfer efficiency is improved, the optimization method not only realizes efficient degradation of the dye, but also effectively overcomes acid inhibition in the anaerobic digestion process under dye stress, and the degradation efficiency of the dye is improved. And quick start and methane recovery of the reactor can be realized without domesticating the sludge.

The invention relates to the technical field of sewage treatment, in particular to a sulfur autotrophic denitrification biological filler and a preparation method thereof, a sulfur autotrophic denitrification fluidized bed, a sulfur autotrophic denitrification filter tank device and application. The sulfur autotrophic denitrification biological filler comprises a macromolecular base material, a sulfur autotrophic denitrification nutrition auxiliary material, a conductive functional auxiliary material and an electrophilic functional auxiliary material in a mass ratio of (90-100): (1-5): (0.5-3): (0.5-3); the macromolecular base material comprises at least one of polyethylene and polypropylene; the sulfur autotrophic denitrification nutrition auxiliary material comprises sulfur; the conductive functional auxiliary material comprises sludge-based biochar; the electrophilic functional auxiliary material comprises cationic starch. The sulfur autotrophic denitrification biological filler has the advantages of being high in mass transfer efficiency, capable of improving the electron transfer efficiency between microbial species, high in biocompatibility and the like.

The invention discloses an anaerobic dynamic membrane bioreactor. The reactor comprises a reactor shell, a cathode, an anode and a power source, the cathode is an electroactive dynamic membrane assembly and comprises a membrane frame, a current collecting net and a conductive net base material, and an electroactive biological membrane selectively formed on a conductive macroporous base material under the dual effects of electric field stimulation and filtering separation serves as a secondary filtering layer. According to the reactor, in a continuous flow mode, the power supply device is used for adjusting the potential of the electroactive dynamic membrane cathode assembly, and an electroactive microorganism dynamic membrane with a specific function is selectively formed, so that degradation and conversion of pollutants in low-organic-strength sewage are accelerated, and improvement of the recovery efficiency and quality purification of biomass gas are realized. Meanwhile, the dynamic membrane component with low price is used as the cathode membrane, so that membrane pollution can be relieved, and the operation cost of the anaerobic membrane bioreactor is reduced.

The invention discloses a CSTR-based three-dimensional electrode MEC-fluidized bed reactor for anaerobic fermentation of cow dung and application, and belongs to the technical field of microbial electrochemistry. The reactor comprises a reactor main body, a feed port, a discharge port, a gas outlet and a stirring device, an anode and a cathode are arranged in the reactor main body, a conductive three-dimensional particle electrode filler is filled between the anode and the cathode to form a three-dimensional electrode system, and the anode and the cathode are connected with an external power supply. The three-dimensional electrode system is constructed, so that the reaction specific surface area and the electron transfer efficiency are greatly increased, the stimulation effect of a low-voltage electric field is amplified to the whole reaction space, and the technical bottlenecks of low mass transfer efficiency, low methane yield, easiness in system instability and the like in the traditional cow dung anaerobic digestion are solved. Compared with the traditional anaerobic digestion and two-dimensional microbial electrolysis cell, the two-dimensional microbial electrolysis cell disclosed by the invention can obviously improve the methane yield and the system operation stability, and has an excellent engineering application prospect.

The invention discloses a dual-MOFs derived composite carbon-based loaded Co-MnO2 catalyst, a preparation method and application, and relates to the technical field of nano catalyst preparation. According to the catalyst, firstly, a Ce-BTC nanorod is synthesized to serve as a carrier, after the carrier is activated through a hydrochloric acid solution, Co < 2 + > ions are immobilized through the electrostatic adsorption effect of carbonyl groups on the surface of the carrier, then a continuous bridging reaction is triggered through the carrier and a 2-methylimidazole ligand, small-size ZIF-67 nanoparticles are formed on the surface of Ce-BTC in situ, and a Ce-BTC ZIF-67 core-shell composite material is obtained; mn < 2 + > ions are doped in an ion exchange mode, and roasting is performed in an inert atmosphere to form the dual-MOFs derivative composite carbon-based loaded Co-MnO2 catalyst (CeO2-C-coated NC / Co-MnO2) with a one-dimensional hollow nano core-shell structure, namely the catalyst disclosed by the invention. The catalyst has good magnetic separation performance, and shows relatively high catalytic activity, selectivity and stability in a catalytic reaction for reducing nitrobenzene substances into amine substances, so that the catalyst has a relatively good application prospect in the field of nano catalysis.

The invention belongs to the technical field of sewage treatment, and particularly relates to a biological filler as well as a preparation method and application thereof. The biological filler comprises a carrier, a denitrification accelerant and a polyphenol-metal network structure, the carrier has a porous structure; the polyphenol-metal network structure is loaded on the surface and inside the carrier, and the polyphenol-metal network structure coats the denitrification accelerant on the surface of the carrier, inside the carrier and in a polyphenol-metal network. Phenolic hydroxyl groups in polyphenol and metal ions are co-deposited, a polyphenol-metal network structure is formed on the surface of the carrier and the surface of the porous structure, and the denitrification accelerant is coated on the surface of the carrier, the pore surface of the porous structure and in the polyphenol-metal network structure by the polyphenol-metal network structure. According to the present invention, the raw material loss and the secondary pollution caused by the dissolution of the denitrification accelerator are avoided, the loading capacity and the loading strength of the denitrification accelerator are improved, the number and the types of the functional groups on the carrier surface are enriched, and the denitrification rate during the biological denitrification process can be further improved.

The invention belongs to the technical field of biochemical engineering, and particularly relates to application of a P450 enzyme mutant and engineering bacteria thereof in synthesis of calcifediol, and the mutant is obtained by mutating one or more sites in an amino acid sequence based on a wild type P450 enzyme Vdh; the amino acid sequence of the wild type P450 enzyme Vdh corresponds to the accession number of GenBank, namely CP069288.1. Through systematic molecular dynamics simulation, conservative analysis and substrate binding pocket engineering on the Vdh enzyme, a plurality of key mutation sites are successfully screened and obtained. The constructed combined mutant (such as I144R / N173M / Q310R, Vdh-M3) shows extremely high hydroxylation activity, the pure enzyme activity of the combined mutant can reach 9.8 times that of a wild type enzyme, and the conversion rate from vitamin D3 to calcifediol is greatly increased.

The invention discloses a preparation method of a biological selective porous iron-sulfur autotrophic denitrification filler. The preparation method comprises the following steps: respectively grinding pyrite and sulfur in a sampling machine to-74 microns, and then carrying out ball milling; carrying out aerobic biological activation on the ball-milled pyrite powder in a 9K basic culture medium, and then carrying out solid-liquid separation to obtain aerobic activated ore; after a fresh culture medium is replaced, sulfur is added, nitrogen is used for blowing air to conduct anaerobic biological activation, then solid-liquid separation is conducted, and anaerobic activated ore is obtained; and cleaning the surface of the obtained anaerobic activated ore with diluted hydrochloric acid, mixing with sodium bicarbonate and activated carbon, ball-milling, pelletizing and sintering at low temperature to obtain the biological selective porous iron-sulfur autotrophic denitrification filler. According to the preparation method of the biological selective porous iron-sulfur autotrophic denitrification filler, the raw materials are wide in source and low in cost, adsorption growth of iron-sulfur autotrophic microorganisms can be selectively promoted, and the ideal effects of advanced sewage treatment and nitrogen and phosphorus removal can be achieved.

This invention discloses a method for improving methanogenesis from lignocellulose based on a mixed-culture fermentation strategy, belonging to the field of anaerobic fermentation technology. The method includes the following steps: (1) inoculating activated *Trichoderma echinosporum* into a culture medium containing lignocellulose and loofah sponge as a biological carrier material for fermentation to obtain a fermentation broth; (2) activating activated sludge using a revitalizing solution; (3) inoculating the activated sludge from step (2) into the fermentation broth of *Trichoderma echinosporum* from step (1) for anaerobic fermentation to produce methanogens. This method achieves a methane yield of 262 mL / g VS, which is more than 30% higher than that of traditional anaerobic fermentation for methanogenesis, and is of great significance for improving the bioconversion of lignocellulose resources.

The invention relates to an immobilized plasma Z-type NiCuBi2O4 / Bi / NiO composite film photocatalyst as well as a preparation method and application thereof. The NiCuBi2O4 film photocatalyst is prepared by taking nickel foil as a substrate and adopting a coprecipitation method, a spin-coating method and a high-temperature calcination method. The NiCuBi2O4 / Bi thin film photocatalyst is prepared through an in-situ reduction method. And finally, preparing the plasma Z-type NiCuBi2O4 / Bi / NiO composite film photocatalyst through a sol-gel method, a spin-coating method and a high-temperature calcination method. The immobilized plasma Z-type NiCuBi2O4 / Bi / NiO composite film photocatalyst with the Ni foil as the substrate is constructed aiming at the problems of agglomeration and recovery in powder photocatalyst reaction, non-noble metal Bi nano particles are introduced to serve as conductive channels, the low-cost plasma resonance effect is achieved, the enhanced catalytic degradation activity is obtained under sunlight, and the photocatalytic degradation effect is improved. Meanwhile, the practical problem that precious metal depends on and powder catalysts are difficult to separate and recover is solved.

The invention belongs to the field of food detection. The invention provides an electrochemical sensor, a preparation method thereof and a method for detecting heavy metal ions in a compound seasoning by using the electrochemical sensor. An electrode material in the electrochemical sensor comprises a composite material obtained by coating an amino-functionalized alkaline MXene material with a nitrogen-doped carbon nanotube. The coordination of an MXene material and heavy metal ions is facilitated through alkalization and amino functionalization, the enrichment of the heavy metal ions is promoted, the electron transfer efficiency of the carbon nanotubes is improved through nitrogen doping, and active sites are provided for the heavy metal ions. The obtained composite material forms a three-dimensional stacked structure and a built-in electric field, provides a larger electrode surface area and stronger electron transfer capability, enhances the detection performance of the obtained electrochemical sensor, has a good linear range, lower detection limit and satisfactory anti-interference capability, repeatability, stability and recovery rate, and can be applied to the field of electrochemical sensors. A new strategy is provided for rapid detection of the heavy metal ions in the compound seasoning.

The invention discloses a biochar electroactive bacterium complex for enhancing degradation of hydrophobic organic pollutants and a preparation method of the biochar electroactive bacterium complex. The method comprises the following steps: (1) pyrolyzing lignocellulose-based biomass under a high-temperature anaerobic condition to obtain original biochar; (2) under an ice bath condition, mixing the original charcoal, NaNO3, concentrated sulfuric acid and KMnO4, carrying out a first oxidation reaction, and keeping continuous stirring; (3) adding a sulfuric acid solution for a second oxidation reaction of the biochar, and after the temperature is reduced by 60 DEG C, adding an H2O2 solution to terminate the reaction; and (4) cleaning the modified biochar material with a mixed solution of sulfuric acid and H2O2, an HCl solution and deionized water until the pH value is neutral, and finally obtaining the modified biochar material. According to the method, the dual advantages of physical adsorption and biodegradation are combined, and an efficient, green and sustainable solution is provided for removal of hydrophobic organic pollutants in a water body.

The invention belongs to the technical field of electrochemical sensors, and relates to a perfluorinated compound electrochemical sensor based on a two-parameter molecular imprinting composite coating as well as a preparation method and application of the perfluorinated compound electrochemical sensor. The perfluorinated compound electrochemical sensor comprises a PFOA molecular imprinting sensing area, a PFOS molecular imprinting sensing area, a glass substrate, a circuit board, an external electrode and the like. [Fe (CN) 6] < 3- / 4-> is taken as a probe molecule, the hole effect on a molecularly imprinted membrane is utilized, that is, a target molecule can be adsorbed to a binding site through electrostatic attraction or hydrogen-bond interaction, after the originally exposed binding site is occupied by the target molecule, the surface conductivity of an electrode is inhibited, a relatively low signal response is generated, and the detection sensitivity is improved. And the concentrations of PFOA and PFOS are quantified according to the change of a current signal generated on the surface of the working electrode. The electrochemical sensor disclosed by the invention is successfully applied to detection of PFOA and PFOS in a water sample, has the advantages of rapid response, high sensitivity, strong specificity, good stability and good anti-interference performance, and can realize simultaneous detection of double targets (PFOA and PFOS) and in-situ cleaning of the electrode in one set of sensor; the volume of the needed sample is obviously reduced, tedious and repeated manual operation is reduced, the cost is low, carrying is easy, the requirement for on-site rapid detection of the actual sample can be met, and marketization is more convenient.

The invention relates to a method and a device for producing medium-chain fatty acid through electric fermentation by using an oxide high-entropy ceramic modified electrode. Through the method and the device provided by the invention, the electrode with high electron transfer efficiency, good biocompatibility, low cost and excellent electrochemical characteristics can be obtained, and the production efficiency of medium-chain fatty acid is improved.

The invention discloses a method for strengthening the denitrification of an Anammox-DAMO coupling system, and belongs to the technical field of sewage microbial denitrification. The method of the invention uses magnetite-loaded activated carbon and growth factors as reinforcing agents to strengthen the denitrification of the Anammox-DAMO coupling system, and has the advantages of small amount of reinforcing agent addition and convenient operation; and can effectively improve the utilization efficiency of methane, strengthen the interspecies electron transfer process of microorganisms, facilitate the coupling of DAMO microorganisms and Anammox bacteria, and improve the growth rate and activity of DAMO microorganisms and Anammox bacteria, and has good application prospects.

The invention belongs to the technical field of biomass dark fermentation hydrogen production, and particularly relates to a method and a device for producing hydrogen by intensifying dark fermentation through thermal modified tourmaline, and the method for producing hydrogen by intensifying dark fermentation through thermal modified tourmaline comprises the following steps: step 1, preparing a dark fermentation process culture medium; 2, dissolving the inoculated sludge into deionized water to prepare sewage, and carrying out heat treatment; step 3, mixing the culture medium, the sewage, the straw and the thermal modified tourmaline to prepare a mixed solution, and performing fermentation preparation; 4, adjusting the pH value of the mixed solution, and starting dark fermentation; and 5, after the primary hydrogen production is ended, adjusting the pH value of the mixed solution again, and starting secondary fermentation until the fermentation is ended. The invention further discloses a thermal modified tourmaline reinforced dark fermentation hydrogen production device applying the method. The tourmaline which is a self-generating field material is applied to dark fermentation, flora reproduction is accelerated by utilizing an electromagnetic enhancement principle, straw biomass can be efficiently degraded, and hydrogen generation is accelerated.

The invention discloses a constructed wetland filler and a preparation method and application thereof, and relates to the field of environmental treatment, and the filler is composed of pyrite quantum dots PQD and carbon dots CDs. The preparation method comprises the following steps: preparing carbon dots CDs into powder, dissolving the powder in distilled water, continuously magnetically stirring and boiling to 100 DEG C, immediately adding pyrite quantum dots PQD of which the mass is equal to that of the carbon dots CDs, stirring and centrifuging under a dark condition, washing with distilled water, and performing vacuum freeze-drying to obtain the PQD-CDs composite material. The filler disclosed by the invention can obviously enhance the pollutant removal efficiency, and has important significance on the treatment of farmland wastewater. The innovative method makes contribution to improvement of efficiency and sustainable development in the field of water treatment.

The application provides an electrochemical aptamer sensor for detecting streptomycin and a preparation method thereof, and the preparation method comprises the following steps: S1, pretreating a glassy carbon electrode; S2, preparing Cu-ZIF-8@AuNPs; performing nitrogen plasma treatment on the Cu-ZIF-8@AuNPs; S3, dropping the plasma modified Cu-ZIF-8@AuNPs suspension on the pretreated glassy carbon electrode and drying; S4, modifying the plasma modified Cu-ZIF-8@AuNPs / GCE composite electrode by using streptomycin aptamer to obtain a modified electrode; S5, performing blocking treatment by using BSA, and then performing washing and drying to obtain the electrochemical aptamer sensor; the plasma modified Cu-ZIF-8@AuNPs can significantly improve the electron transfer efficiency, and can enhance the streptomycin detection sensitivity, selectivity and stability of the electrochemical aptamer sensor.