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Mixed flora and purpose thereof, microbial electrogenesis system containing mixed flora, and microbial fuel cell

A technology of mixed flora and microorganisms, applied in biochemical fuel cells, microorganisms, methods based on microorganisms, etc., can solve the problems of complex system, poor system stability and repeatability, high cost, etc., and achieve the goal of simple system and enhanced electron transfer efficiency Effect

Active Publication Date: 2017-05-31
TIANJIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The stability and repeatability of the system are poor, the duration of power generation is short, and dozens of mineral and vitamin solutions need to be added, which is costly and complicated

Method used

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  • Mixed flora and purpose thereof, microbial electrogenesis system containing mixed flora, and microbial fuel cell
  • Mixed flora and purpose thereof, microbial electrogenesis system containing mixed flora, and microbial fuel cell
  • Mixed flora and purpose thereof, microbial electrogenesis system containing mixed flora, and microbial fuel cell

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0062] Embodiment 1 mixed flora

[0063] Construction of Bacillus subtilis with high riboflavin production: Through genetic manipulation, the prs and ywlF genes in Bacillus subtilis were overexpressed, and the Pur operon and PurR regulatory genes (glyA, guaC, pbuG, xpt-pbuX, yqhZ-folD, and pbuO).

[0064] Construct Escherichia coli that produces small molecular acids using five-carbon sugars and six-carbon sugars as carbon sources: use λ-Red homologous recombination technology to knock out the pflB gene in Escherichia coli, and then introduce the ldhE gene (lactic acid-producing gene, derived from Lactobacillus, Synthesized by GENWIZE company), the method is: by EcoRI and PstI digestion, connected to the pSB1C plasmid, and then the plasmid connected with the ldhE gene is introduced into the above-mentioned Escherichia coli with the pflB gene knocked out, and the chloramphenicol resistance is screened, and the correct transformants. Take the constructed Bacillus subtilis with...

Embodiment 2

[0067] Embodiment 2 mixed flora

[0068] Construction of Bacillus subtilis with high riboflavin production: Through genetic manipulation, the prs and ywlF genes in Bacillus subtilis were overexpressed, and the Pur operon and PurR regulatory genes (glyA, guaC, pbuG, xpt-pbuX, yqhZ-folD, and pbuO).

[0069] Construct Escherichia coli that produces small molecular acids using five-carbon sugars and six-carbon sugars as carbon sources: use λ-Red homologous recombination technology to knock out the pflB gene in Escherichia coli, and then introduce the ldhE gene (lactic acid-producing gene, derived from Lactobacillus, Synthesized by GENWIZE company), the method is: by EcoRI and PstI digestion, connected to the pSB1C plasmid, and then the plasmid connected with the ldhE gene is introduced into the above-mentioned Escherichia coli with the pflB gene knocked out, and the chloramphenicol resistance is screened, and the correct transformants.

[0070] Take the constructed Bacillus subt...

Embodiment 3

[0073] Embodiment 3 mixed flora

[0074] Construction of Escherichia coli with high riboflavin production 1: Introducing the ribABDEC gene cluster into Escherichia coli;

[0075] Construction of Escherichia coli 2 that uses five-carbon sugars and six-carbon sugars as carbon sources to produce small molecular acids: the pflB gene was knocked out in Escherichia coli using λ-Red homologous recombination technology, and then the ldhE gene (lactic acid-producing gene, derived from Lactobacillus , synthesized by GENWIZE company), the method is: digested with EcoRI and PstI, connected to the pSB1C plasmid, and then the plasmid with the ldhE gene is introduced into the above-mentioned Escherichia coli with the pflB gene knocked out, and the resistance to chloramphenicol is screened to screen out correct transformants.

[0076] Take the constructed Escherichia coli 1 with high riboflavin production, the constructed Escherichia coli 2 that uses five-carbon sugars and six-carbon sugars ...

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Abstract

The invention relates to the field of microbial power generation, in particular to a mixed flora and a purpose thereof, a microbial electrogenesis system containing the mixed flora, and a microbial fuel cell. Through construction of an engineered mixed flora, the characteristic of definite division of labor is realized; zymophyte 1 provides carrier riboflavin of electron transfer for the system; and zymophyte 2 converts most common cheap glucose into a small molecule acid and provides the small molecule acid for electrochemically active microorganisms, so that the spectrum of carbon sources of the electrochemically active microorganisms is expanded. According to the mixed flora and the purpose thereof, the microbial electrogenesis system containing the mixed flora and the microbial fuel cell, two zymophytes with different purposes are constructed by performing methods, such as genetic engineering alteration, on type strains of escherichia coli or bacillus subtilis-protokaryotic gram negative bacteria and gram-positive bacteria; and therefore, the spectrum of carbon sources of the microbial fuel cell is expanded and the electron transfer efficiency of the electrochemically active microorganisms is enhanced. A stable, reasonable and efficient functional mixed flora compound electrogenesis system is constructed from three important angles of a material flow, an energy flow and an information flow.

Description

technical field [0001] The invention relates to the field of microbial electricity generation, in particular to mixed flora and its application, a microbial electricity generation system containing the mixed flora and a microbial fuel cell. Background technique [0002] Microbial Fuel Cell (MFC) is a device that uses microorganisms to directly convert chemical energy in organic matter into electrical energy. Its basic working principle is: in the anaerobic environment of the anode chamber, the organic matter decomposes under the action of microorganisms and releases electrons and protons. It is effectively transmitted between the anode and the electrode, and is transmitted to the cathode through an external circuit to form a current, while the proton is transmitted to the cathode through the proton exchange membrane, and the oxidant gets electrons at the cathode and is reduced and combined with the proton to form water. [0003] Compared with other existing technologies tha...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/21C12N1/20H01M8/16C12R1/125C12R1/19C12R1/01
CPCY02E60/50Y02P70/50
Inventor 元英进刘悦赵学明陈涛白雪
Owner TIANJIN UNIV
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