The invention relates to the field of microbial power generation, in particular to a mixed flora and a purpose thereof, a microbial electrogenesis system containing the mixed flora, and a microbial fuel cell. Through construction of an engineered mixed flora, the characteristic of definite division of labor is realized; zymophyte 1 provides carrier riboflavin of electron transfer for the system; and zymophyte 2 converts most common cheap glucose into a small molecule acid and provides the small molecule acid for electrochemically active microorganisms, so that the spectrum of carbon sources of the electrochemically active microorganisms is expanded. According to the mixed flora and the purpose thereof, the microbial electrogenesis system containing the mixed flora and the microbial fuel cell, two zymophytes with different purposes are constructed by performing methods, such as genetic engineering alteration, on type strains of escherichia coli or bacillus subtilis-protokaryotic gram negative bacteria and gram-positive bacteria; and therefore, the spectrum of carbon sources of the microbial fuel cell is expanded and the electron transfer efficiency of the electrochemically active microorganisms is enhanced. A stable, reasonable and efficient functional mixed flora compound electrogenesis system is constructed from three important angles of a material flow, an energy flow and an information flow.