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Enzyme-free electrochemical immunodetection system for KIM-1 protein and preparation and use method of enzyme-free electrochemical immunodetection system

A KIM-1 and immunoassay technology, applied in the field of protein detection, can solve the problems of high detection cost, complicated operation, and poor specificity

Active Publication Date: 2020-11-27
AFFILIATED HOSPITAL OF ZUNYI UNIV
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  • Application Information

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Problems solved by technology

[0004] In view of the shortcomings of the prior art described above, the object of the present invention is to provide an enzyme-free electrochemical immunoassay system for KIM-1 protein, which is used to solve the problems of high detection cost, complicated operation, and low sensitivity in the prior art. poor specificity

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  • Enzyme-free electrochemical immunodetection system for KIM-1 protein and preparation and use method of enzyme-free electrochemical immunodetection system
  • Enzyme-free electrochemical immunodetection system for KIM-1 protein and preparation and use method of enzyme-free electrochemical immunodetection system
  • Enzyme-free electrochemical immunodetection system for KIM-1 protein and preparation and use method of enzyme-free electrochemical immunodetection system

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[0042] The following is further described in detail through specific implementation methods:

[0043] The English abbreviations in this application correspond to the Chinese respectively:

[0044] PdPtBP: palladium platinum boron phosphorus;

[0045] PdPtBP MNPs: PdPtBP mesoporous nanoparticles;

[0046] Ti 3 C 2 T X MXene: thin layer of titanium carbide;

[0047] Ti 3 C 2 T X MXene-NH 2 : Amino-functionalized Ti 3 C 2 T X MXene;

[0048] Ti 3 C 2 T X MXene dispersion: titanium carbide thin layer dispersion;

[0049] CuCl 2 NWs: copper chloride nanowires;

[0050] PdPtBP MNPs / MXene: PdPtBP mesoporous nanoparticles / mycoene nanocomposite;

[0051] AuNPs / CuCl 2 NWs / GCE: CuCl 2 Electrodes decorated with nanowires and gold nanoparticles;

[0052] Ab1: capture antibody;

[0053] Ab2: labeled antibody;

[0054] The capture antibody Ab1 and the labeled antibody Ab2 are two monoclonal antibodies, both of which can recognize and bind to the antigen to be te...

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Abstract

The invention discloses an enzyme-free electrochemical immunodetection system for KIM-1 protein in the technical field of protein detection, which comprises an upper-layer compound and a lower-layer compound, wherein the upper-layer compound is a PdPtBP mesoporous nano-particle / MXene nano-compound prepared by combining PdPtBP mesoporous alloy nano-particles and a Ti3C2TX MXene dispersion liquid, and a labeled antibody is combined on the PdPtBP mesoporous nano-particle / MXene nano-compound; the lower-layer compound is a modified electrode, the modified electrode is an electrode modified by CuCl2nanowires and gold nanoparticles, and a capture antibody is combined on the modified electrode; and the detection system is a PBS solution containing H2O2. According to the scheme, enzyme-free detection of protein is realized, and the problems of high detection cost, complex operation, low sensitivity, poor specificity and the like in the prior art are solved.

Description

technical field [0001] The invention belongs to the technical field of protein detection, in particular to an enzyme-free electrochemical immunological detection system for KIM-1 protein and its preparation and use method. Background technique [0002] Acute kidney injury (AKI) is a clinical inpatient disease with high treatment cost and high mortality. Currently, the standard diagnostic tools for AKI are monitoring of urine output and serum creatinine (sCr). However, both are markers of kidney function, not kidney damage. In addition, because urine output and sCr are easily affected by factors such as muscle mass, age, sex, and diet, the utility of urine output and sCr in the diagnosis of AKI is limited. Recent evidence indicates that urinary kidney injury molecule-1 (KIM-1) protein is a potential marker of AKI, which can rapidly and noninvasively reflect the process of kidney injury and recovery. Therefore, the development of a reliable and highly sensitive KIM-1 protei...

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Application Information

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IPC IPC(8): G01N27/327G01N27/30G01N33/543G01N33/68
CPCG01N27/3277G01N27/3278G01N27/308G01N33/54346G01N33/5438G01N33/6893G01N2800/347Y02A50/30
Inventor 刘长金丁世家文静闵迅韩道滨
Owner AFFILIATED HOSPITAL OF ZUNYI UNIV
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