N-terminal brain natriuretic peptide precursor detection kit and detection method thereof

A technology for detection kits and brain natriuretic peptides, applied in biological testing, measuring devices, material testing products, etc., can solve the problem that latex immune turbidimetry is difficult to use with automatic biochemical analyzers.

Active Publication Date: 2018-10-02
SHAOXING SHENG KANG BIOLOGICAL TECH
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Problems solved by technology

[0011] However, no ideal sensitized latex particles have been found for the detection of N-terminal BNP precursors. The present invention solves such a problem; at present, it is difficult for latex immunoturbidimetry to be used in combination with a fully automatic biochemical analyzer in clinical practice. Batch samples, and the results are mostly qualitative; the present invention solves such problems

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  • N-terminal brain natriuretic peptide precursor detection kit and detection method thereof
  • N-terminal brain natriuretic peptide precursor detection kit and detection method thereof
  • N-terminal brain natriuretic peptide precursor detection kit and detection method thereof

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Embodiment Construction

[0114] The present invention will be specifically introduced below in conjunction with the accompanying drawings and specific embodiments.

[0115] A detection kit for N-terminal BNP precursor, comprising:

[0116] Reagent one:

[0117] Phosphate buffer (PH=6.5) 100-150mmol / L,

[0118] Preservatives 0.005%-0.06%,

[0119] Tween 20 0.5-2.0mL / L,

[0120] Polyethylene glycol 6000 25-60g / L;

[0121] Reagent 2:

[0122] Phosphate buffer (PH=8.0) 100-150mmol / L,

[0123] Disodium hydrogen phosphate 20-60g / L,

[0124] Sodium dihydrogen phosphate 2-5g / L,

[0125] Sodium chloride 10-50g / L,

[0126] Preservatives 0.005%-0.06%,

[0127] Sensitized latex particles of goat anti-human N-terminal-pre-B-type natriuretic peptide antibody 1-5ml / L;

[0128] Calibrator:

[0129] Phosphate buffer (PH=8.0) 100-150mmol / L,

[0130] Bovine serum albumin 10-50g / L,

[0131] Sodium chloride 10-50g / L,

[0132] Preservatives 0.005%-0.06%,

[0133] N-terminal-pre-B-type natriuretic peptide ant...

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Abstract

The invention discloses an N-terminal brain natriuretic peptide precursor detection kit and a detection method thereof. The kit comprises: reagents I: a phosphate buffer solution, a preservative, Tween 20 and polyethylene glycol; reagents II: a phosphate buffer solution, disodium hydrogen phosphate, sodium dihydrogen phosphate of 2 to 5 g / L, sodium chloride, a preservative and sensitized latex particles of a goat anti-human N-terminal-front B type natriuretic peptide antibody; and a calibration product: a phosphate buffer solution, bovine serum albumin, sodium chloride, a preservative and N-terminal-front B type natriuretic peptide antigen. According to the method, the test sensitivity is improved by applying a latex immunoturbidimetric method, the sensitized latex particles suitable for detecting an N-terminal front B type natriuretic peptide precursor are found, and the detection sensitivity is improved; large-batch samples can be detected quantitatively and simultaneously; and the kit comprises the calibration products and the quantitative detection aim is fulfilled.

Description

technical field [0001] The invention relates to the field of in vitro diagnosis, in particular to an N-terminal brain natriuretic peptide precursor detection kit and a detection method thereof. Background technique [0002] In vitro diagnostic reagents refer to: they can be used alone or in combination with instruments, appliances, equipment or systems, and are used in the process of disease prevention, diagnosis, treatment monitoring, prognosis observation, health status evaluation and genetic disease prediction. Reagents, kits, calibrators (materials), quality control products (materials), etc. for in vitro detection of samples (various body fluids, cells, tissue samples, etc.). [0003] According to the level of product risk, in vitro diagnostic reagents are divided into the third category, the second category, and the first category of products. The third category of products: reagents related to the detection of pathogenic pathogen antigens, antibodies, and nucleic aci...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/82G01N33/68
CPCG01N21/82G01N33/6893G01N2021/825G01N2800/32
Inventor 黄争上吕伟军俞媛媛杨春超
Owner SHAOXING SHENG KANG BIOLOGICAL TECH
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