Biotin-containing monomers, polymeric materials formed from the
biotin-containing monomers, articles containing the polymeric materials, methods of making the articles, and methods of using the articles are provided. The articles can be used, for example, for affinity capture of
biotin-binding proteins, including
biotin-binding fusion proteins (i.e., a biotin-
binding protein fused to another
biomaterial). Articles that contain captured biotin-binding proteins can be further used for affinity capture of various biotin-containing biomaterials such as biotinylated proteins. The articles can also be used, for example, for affinity capture of biotin-binding fusion proteins where the
fusion protein includes, for example, an
enzyme or
antibody.