59results about How to "Does not affect vitality" patented technology

The invention discloses aryl formyl amino cyclopropanecarboxylic acid serving as a novel plant growth regulator. The compound provided by the invention is shown as a general formula I, wherein in the formula I, R may be R1 or R2; R1 may be phenyl or a benzene ring containing a substituent group; R2 may be pyridyl or a pyridine ring containing a substituent group; and the substituent groups in the benzene ring containing the substituent group or the pyridine ring containing the substituent group may be one or any two of halogen, NO2, alkyl and alkoxy. Experiments prove that the aryl formyl amino cyclopropanecarboxylic acid compound synthesized by the invention has the plant growth regulation activity of inhibiting seed germination by the measurement on the germination activity of wheat seeds.

The invention provides an expansion in vitro purification culture method of mesenchymal stem cells and a cell culture medium used in a culture process. According to the method, mesenchymal stem cells differentiate to osteogenic stem cells. The cell culture medium comprises the following components: 8-12mu g / L of bFGF (Basic Fibroblast Growth Factor), 8-12mu g / L of EGF (Epidermal Growth Factor), 8-12mu g / L of PDGF-BB (Platelet Derived Growth Factor-BB), 10 percent of fetal calf serum, 100U / mL of penicillin and 100mg of L of streptomycin; a solvent is a basic culture medium; and the basic culture medium is a DMEM (Dulbecco Modified Eagle Medium) culture solution, an F12 culture solution or a mixture of the DMEM culture solution and the F12 culture solution. The mesenchymal stem cells obtained by adopting a method comprising the steps of early replacement of the cell culture medium, cell adherence passage and combined addition of cell factors (bFGF, EGF and PDGF-BB) in the culture solution are high in purity and strong in vitro proliferation capacity, and have good capability of differentiation to osteogenic stem cells.

The invention relates to a method for sorting target cells by using avidin / streptavidin magnetic composite particles. In the method, a biotin-labeled monoclonal antibody is added into an experimental system, magnetic particles are bonded with the biotin-labeled monoclonal antibody through avidin / streptavidin, the monoclonal antibody is specifically bonded with corresponding antigen on the surfaceof the cells to make the cells captured by the magnetic particles so as to purify and separate the cells. Because the biotin-avidin and biotin-streptavidin have the characteristics of high system specificity, high stability, wide application range and low experiment cost, the obtained avidin / streptavidin magnetic composite particles have high biotin bonding property; and the labeled amount is improved in the measurement technique, and biomolecules are difficult to denature or inactivate, so that the cell sorting specificity is further improved. The high-purity target cells can be sorted by specifically bonding the biotin with the avidin or streptavidin; and the method has the advantages of no need of expensive instrument, simple operation and time saving.

The present invention is technological scheme of magnetic metal particle process for separating out target cell from cell mixture. The process includes the following steps: 1. fixing monoclonal antibody molecule onto the surface of magnetic metal particle; 2. incubating the magnetic metal particle with fixed monoclonal antibody molecule and the cell mixture to be separated for 15-60 min for combining the cell surface antigen to the monoclonal antibody and combining the cell onto the surface of magnetic metal particle; 3. magnetically separating the target cell from other cells in a magnetic separator; and 4. washing the magnetic metal particle to eliminate non-specifically combined cells. The process has the advantages of short time, low cost, low cell toxicity, no influence on cell activity, etc.

The invention discloses a human osteosarcoma stem-cell related antigenic marker stage-specific embryonic antigen-4 (SSEA-4) and an application thereof in a targeted therapy of osteosarcoma. The SSEA-4 is a specific osteosarcoma stem-cell surface marker which is almost not expressed in each normal adult tissue. The surface marker is easy for antibody labeling without affecting the cell viability, and applicable to the function tests after separation, therefore, a reliable molecular tracer is provided for further researching the function of stem cells in the origin, occurrence and development processes of osteosarcoma, and a foundation for explaining the clinical osteosarcoma drug resistance as well as transferring and looking for cellular and molecular targets for the targeted therapy is laid.

The invention relates to a method for producing paper pulp by adopting cotton stalk peel as raw material, which adopts an enzyme-ammonium sulfite method. The enzyme-ammonium sulfite method comprises the steps of: firstly, removing pectin of the cotton stalk peel and degrading lignin by using degumming enzyme and then, preparing coarse pulp by an ammonium sulfite boiling step, and finally processing the coarse pulp by a conventional production method to obtain finished product pulp, wherein the degumming enzyme is prepared by compounding pectinase, laccase and lignin enzyme in the weight ratio of 1-3:1:1, and a degumming enzym solution is prepared by compounding the degumming enzyme, urea and water in the weight ratio of 5:5:1000-15:15:1000; the cotton stalk peel is soaked in the sealed degumming enzyme solution for 24-48 hours in the static state at the temperature of 20-30 DEG C. The weight ratio of the ammonium sulfite to the oven dry cotton stalk peel in a boiling solution is 10-13 percent, and the pH value of the boiling solution is 7-9. In the invention, the pectinase is added in the degumming enzyme, which effectively remove pectic substances and solves the problem that subsequent processing is influenced by the existence of the pectin and industrialized production can not be realized. The invention can produce the paper pulp meeting standards. Compared with the traditional chemical pulp producing method, the invention has the advantages of high pulp yield, energy saving, cost reduction and recycled black liquor.

The invention provides a TAT (trans-activating factor) kringle domain-modified nerve transcription factor nenurogenin2 fusion protein, as well as a preparation method and application thereof. Specifically, the fusion protein TAT-nenurogenin2 formed by the nerve transcription factor nenurogenin2 is modified by the protein transduction kringle domain of the human immunodeficiency virus trans-form activation transduction protein TAT. The preparation of the fusion protein provided by the invention comprises the following steps of: (1) selecting a TAT protein transduction domain-containing pTAT-HA expression vector; (2) synthesizing the nerve transcription factor nenurogenin2 target gene segment optimized by prokaryotic expression; (3) connecting T4 ligase with the enzyme-digested product to obtain a pTAT-nenurogenin2 recombinant expression plasmid vector by means of cloning and screening; and (4) transforming the recombinant expression plasmid vector into colon bacillus DE3 competent cell, and inducing protein expression. According to the invention, a large number of TAT-nenurogenin2 fusion protein which is low in cost, high in activity, and capable of inducing the astrocyte to be transformed into the nerve cell with biological activity can be firstly prepared, and the TAT-nenurogenin2 is easy to penetrate through the blood brain barrier (BBB), so that the TAT kringle domain-modified nenurogenin2 fusion protein is wide in the prospect in the clinical treatment of the brain injury disease.

The invention relates to a beauveria bassiana seed coating suspoemulsion which comprises suspoemulsion 1.0 g and corn seeds 50-100 g. The suspoemulsion and the corn seeds are mixed evenly and aired, and the aired corn seeds waiting for being sown. The reciprocal symbiosis relationship between beauveria bassiana and plants is utilized, the seed coating which is high in spore content of coated seeds, high in spore germination rate and capable of being efficiently sown in a mechanized mode is screened, the seed coating can obviously promote the growth of corn and improve the insect resistance of the corn, the growth promotion effect and the insect-resistant effect on the corn are achieved, and mechanized sowing can be achieved.

The invention discloses a method for AMF spore surface disinfection, with the technical scheme comprising: putting filter paper and absorbent paper respectively inside several culture dishes; preparing spore surface antiseptic solution; putting the filter paper carrying AMF spores between the double-layer filter paper inside the culture dish; absorbing the antiseptic solution for dropping on the upper filer paper in a dropwise manner; shifting the double-layer filter paper inside the culture dish with filter paper for absorbing the adsorbed antiseptic solution via the filter paper; taking outthe upper filter paper and putting the upper filter paper inversely inside another culture dish, and transferring the AMF spores on the filter paper, thereby completing the surface disinfection. The invention realizes the surface disinfection of AMF spores through the combination of double-layer filter paper and culture dishes. The method is easy and mild to operate, consumes relatively low amount of antiseptic solution, and causes no loss of spores before or after disinfection, without affecting the vitality of the spores and without the assistance of other apparatus.

The invention relates to a seed cracker, more particularly to a seed cracker of Cassia sophera. The seed cracker comprises a base, a drive assembly and a cracking assembly, and a feed and discharge assembly required by the seed cracker, wherein the drive assembly and the cracking assembly are arranged on the base. By arranging the cracking assembly which comprises a guide rod, a grinding wheel and fan-shaped blades that are mutually matched, cone-shaped bumps on the blades are in contact with seeds to break shells of the seeds, thereby the processing efficiency is increased to a great extent.In addition, because resonance is not easy to occur, the noise during operation is smaller.

The invention belongs to the technical field of detection of medical samples, and in particular relates to a mycobacterium tuberculosis sputum specimen liquefier kit and application thereof. The mycobacterium tuberculosis sputum specimen liquefier kit includes a liquefier R1 and a liquefier R2; and the liquefier R1 is ammonia, and the liquefier R2 is a water solution of a sulfydryl reducing agent. The invention also provides application of the above mycobacterium tuberculosis sputum specimen liquefier kit, and the kit can be applied to acid-fast staining microscopic examination of mycobacterium tuberculosis and liquefaction of sputum specimen before isolated culture. Compared with the prior art, the technical scheme provided by the invention has the following advantages: the kit helps to improve smear quality, increases positive detection rate and reading efficiency, optimizes the existing pretreatment technology for sputum specimens, satisfies requirements of multiple detection samples, and is in favor of staining microscopic examination of the sputum specimen smear and automation operation of isolated culture and inoculation.

The invention discloses a seed coating agent for promoting germination and seedling formation of leguminous seeds in saline-alkali soil and a preparation method of the seed coating agent. The seed coating agent mainly comprises a soybean rhizobium solution, a bacillus liquid a, a bacillus liquid b, silk fibroin, peptone and the like. A film is formed outside the seeds before sowing, so that the seeds are helped to resist abiotic stress outside soil, and a protective layer can be formed around rhizosphere during seedling growth, so that the harm of abiotic stress to the seedlings is further reduced, and the leguminous seeds can grow healthily.

The invention discloses application of ganoderma extracts and ganoderma triterpenes monomers for treating autosomal dominant inheritance polycystic kidney diseases. The ganoderma extracts are prepared through a preparation method of ganoderma extracts. The method comprises the steps that an ethanol water solution is used for backflow to extract ganoderma lucidum to obtain an ethanol extract; ethyl acetate is used for extracting the ethanol extract, an ethyl acetate phase is collected to obtain an ethyl acetate extract; a sodium bicarbonate water solution is used for extracting the ethyl acetate extract, and a water phase is collected to obtain a sodium bicarbonate extract; the ethyl acetate is used for extracting the sodium bicarbonate extract, and an ethyl acetate phase is collected to obtain the ganoderma extracts; the ganoderma triterpenes monomers are ganoderic acid C2 ethyl ester, lucidone D or / and ganoderic acid C2. An experiment proves that the ganoderma extracts and the ganoderma triterpenes monomers can restrain formation and growth of kidney vesicae, and can be used for treating the autosomal dominant inheritance polycystic kidney diseases.

The invention discloses a preparation method for TAT-NEP1-40 fusion protein and application in treating the injuries of a central nervous system, CNS. The TAT-NEP1-40 fusion protein is characterized in that the TAT-NEP1-40 fusion protein comprises the protein transduction domain (PTD) of a transactivating transduction protein TAT of the human immunodeficiency virus (HIV) and Nogo extracellular peptide residues 1-40, NEP1-40. The fusion protein retains the selective antagonistic activity of the NEP1-40 to a Nogo receptor, NgR, and has the advantages of high transduction efficiency and easy penetration of BBB and BCSB, thereby overcoming the limitation of the NEP1-40, avoiding the extra injuries on nervous tissues caused by the mode of injecting the NEP1-40 through microinjection or miniature pump implantation, and overcoming the problem that the NEP1-40 can hardly pass BBB and BCSB to reach corresponding biological concentration. The fusion protein can be prepared in a large quantity, has a low cost and high activity, and can be used for treating a plurality of CNS injuries including cerebral ischemia, cerebral anoxia, cerebral hemorrhage, cerebral trauma, spinal core injury, and the like, and promoting the regeneration and functional rehabilitation of nervous tissues.

The invention discloses a compound enzyme and plant extract two-in-one type deodorant formula and a preparation method. The formula includes, by weight, 2.0 to 7.5 parts of plant essential oil, 15.0 to 29.0 parts of a plant extract composition, 10.0 to 23.0 parts of a compound enzyme composition, 1.8-5.2 parts of alkyl glycoside, 9.5-10.0 parts of water-soluble chitosan quaternary ammonium salt, 3.0-10.0 parts of n-octanol and 30.0-250.0 parts of deionized water. The deodorant can effectively absorb and transform ammonia gas and organic amine compounds in air, and can efficiently absorb and convert malodorous gas molecules such as nitrogen compounds and sulfur compounds into non-toxic, harmless and odorless substances. The deodorant is safe, green and efficient, does not generate secondarypollution, and realizes 'zero emission'.

The invention discloses a united preparation technology of earthworm elastic (collagen) protein and lumbrukinase. Earthworms are used as raw materials, by a freeze-etching method, a crust layer and anelastin layer are thoroughly separated, and collected earthworm exudate is used as pulp and can be used for producing the lumbrukinase; and the surplus parts are subjected to processing by a countercurrent flow suspension method for refining of elastic collagen protein, and then through the steps of glue cooking, decolorization, membrane separation, concentration, freeze drying and the like, theearthworm elastic collagen protein is obtained. According to the united preparation technology disclosed by the invention, the utilization degree of the earthworms is increased, no additive componentsare used in the process of producing the earthworm elastic collagen protein and the lumbrukinase, the quality of the earthworm elastic (collagen) protein and the lumbrukinase is increased, the production cost of the earthworm elastic (collagen) protein and the lumbrukinase is reduced, besides, the preparation technology is simple, the vitality of the lumbrukinase cannot be influenced, and industrialized production can be realized.

The invention discloses a post-harvest treatment and storage method for a potato minituber produced by a fog culture method, relates to the post-harvest treatment and storage method for the potato minituber and requirements of the potato minituber on an environment during treatment and storage, and mainly solves the problem of difficulty in storage of the potato minituber produced by a fog culture technology or a water culture technology. The post-harvest treatment and storage method comprises the following steps: after harvesting a tuber, timely killing various pathogens on the surface of the tuber, and creating proper environmental conditions for quickly healing wounds on the surface of the tuber, quickly suberifying epidermis, increasing solanine content and laying a foundation for improving resistance to pest and disease damage during storage, keeping freshness and vitality of the minituber and greatly prolonging the storage period; and storing the treated minituber for a long time in a low-temperature high-humidity storage facility. Compared with the conventional storage method, the post-harvest treatment and storage method has the advantages as follows: pest and disease damage is basically not caused during storage, the storage period of the tuber is greatly prolonged to be about 18 months, the water loss of the tuber is very low, and the freshness and the vitality of the minituber are maintained.

The invention relates to a method for preparing S-ademetionine (SAM), in particular to a method for separating S-ademetionine from a yeast fermentation liquor, and belongs to the field of functional foods and preparation methods thereof. The method for separating S-ademetionine from the yeast fermentation liquor comprises the following steps: filtering the yeast fermentation liquor, performing centrifugation on the filtrate, pretreating collected substrate yeast, performing cell disruption treatment according to an extruded cell disruption method, carrying out centrifugation to obtain a supernatant, adding an acetone solvent, carrying out filtering, collecting a precipitate, and freeze-drying at low pressure to obtain the S-ademetionine. According to the preparation method provided by the invention, the disruption rate of yeast cells can reach about 85%, and the extraction rate of the S-ademetionine can reach more than 75%; the preparation method has the characteristics of being simple in process, high in operability, suitable for industrial production and high in recovery rate.

The invention discloses a PEG modified nucleic acid aptamer as well as a preparation method and application thereof. PEG modification is performed by aiming at a GCRV (grass carp reovirus) nucleic acid aptamer; modification is performed by using different molecular weights of PEG at different reaction time and different reaction proportions; the optimal reaction conditions are screened; the cell viability is not influenced; the inhibition effect is achieved on the GCRV infection. The modified GCRV nucleic acid aptamer has application prospects for preparing viral disease treatment medicine, and also has application prospects for preparing GCRV molecular probes and detection reagents.

The invention relates to a dendritic cell-targeted hybrid dendrimer / YTHDF1 siRNA compound as well as a preparation method and application thereof. The preparation method comprises the following steps: preparing G5.NH<2>-Man; preparing G5.NH<2>-Man-PS; preparing {(Au<0>)<25>-G5.NH<2>-Man-PS<20>}DENPs; and preparing the dendritic cell-targeted hybrid dendrimer / YTHDF1 siRNA compound. The method is simple in operation process and mild in reaction conditions; and the prepared compound can be efficiently swallowed by dendritic cells without influencing the activity of the dendritic cells, can transfect the dendritic cells and silence the expression of YTHDF1, and is used for enhancing tumor immunotherapy.

The invention belongs to the field of modern pharmacy of traditional Chinese medicines, and relates to application of a specific monomer leonurine in preparation of a medicine for improving infertility pregnancy outcome. The leonurine is injected into the abdominal cavity during an EMS (acute renal syndrome) modeling period, so that the volume and weight of an ectopic focus can be remarkably reduced, local fibrosis, invasion and transfer are inhibited, and the pregnancy rate of an EMS model mouse can be remarkably increased. The leonurine inhibits proliferation and invasion of endometrium stromal cells by inducing apoptosis of the endometrium stromal cells, adjusts expression balance of a local estrogen-progestin receptor of the ectopic focus, activates EMS model pelvic and abdominal immune cells, enhances phagocytic and killing functions of the cells, reverses poor ecdysis of the EMS model mouse, has similar effects on growth and invasion of ectopic focus ESC of a human EMS patient and expression of the estrogen-progestin receptor, does not affect growth and activity of the endometrium ESC of a normal child-bearing female, hormone receptor expression, ecdysis process and proliferation, apoptosis and cell activity of human early-pregnancy embryo trophoblast, and can be used for preparing an effective prevention and treatment medicine for EMS combined infertility patients.

The invention discloses a preparation method of a porphyrin photosensitizer with visible photodynamic therapy properties. The method comprises reacting water-soluble porphyrin molecules and soluble nitrates under the conditions of acidity, light avoidance and room temperature to obtain a porphyrin photosensitizer containing The reaction system of the porphyrin photosensitizer, after the system is processed, the porphyrin photosensitizer is obtained, which is a nano-aggregate and has the characteristics of cell imaging and photodynamic therapy. In addition, the preparation method provided by the present invention has a simple process, does not require complicated synthesis steps and post-processing steps, is easy to operate, and has relatively low cost, and therefore, is easy to popularize in production.

The invention discloses a breeding box. The breeding box is characterized in that the breeding box comprises a first classification box, a second classification box and a third classification box which are in sequential suit installation from top to down; the volume of the three classification boxes increases in turn, and openings are opened on the upper ends of all the three classification boxes; the opening of the second classification box is set to be adapted to the size of the bottom of the first classification box; the opening of the third classification box is set to be adapted to the size of the bottom of the second classification box; the first classification box extends out from the opening of the second classification box to be fixedly mounted in the second classification box; afirst fish barrel horizontally running through the second classification box is disposed on the bottom end of the side wall of the first classification box; both sides of the upper ends of both the second classification box and the third classification box are provided with hinged sealing cover plates; only a valve body needs opening to let out all water when breeding is completed and fish need taking out; to remove the fish from the three boxes in an orderly manner, only the sealing cover plates need opening, and thus, the breeding box is easy to use.

The invention discloses a propagule (including individual, organ, tissue, cell and other biological sexual, asexual and parthenogenetic propagation materials, maternal, young, ovum and germ cells, and animal tissue, cell, spore, hypha, sporocarp and other sexual or asexual propagation fungus materials for division reproduction, germination reproduction, rupture reproduction and the like) extract and an application thereof. The propagule extract is obtained by extracting a required propagule; when in use, the extract is uniformly mixed with a solvent to prepare a dormancy period prolonging agent. The surface of the propagule can be covered with a storage life prolonging agent to form a film by means of spraying, soaking, brushing and the like, the treated propagule can be stored by means of packaging, sustained-release microcapsules and the like after being aired, or the storage life prolonging agent is combined with other storage methods to prolong the dormancy period. According to the propagule (including the individual, the organ, the tissue, the cell and the other biological sexual, asexual and parthenogenetic propagation materials, the maternal, the young, the ovum and the germ cells, and the animal tissue, the cell, the spore, the hypha, the sporocarp and the other sexual or asexual propagation fungus materials for division reproduction, germination reproduction, rupture reproduction and the like) extract and the application thereof, The dormancy period of the propagule can be obviously prolonged, the reproductive capacity of the propagule subjected to activating treatment is recovered, and the development of the subsequent propagule is not influenced.

The invention relates to an application of Resistin bind polypeptide to prepare the drug that accelerates insulin secretion, wherein the invention provides a relative application method, to clear the function of polypeptide.

The invention relates to a seed cracker, more particularly to a seed cracker of Cassia sophera. The seed cracker comprises a base, a drive assembly and a cracking assembly, and a feed and discharge assembly required by the seed cracker, wherein the drive assembly and the cracking assembly are arranged on the base. By arranging the cracking assembly which comprises a guide rod, a grinding wheel and fan-shaped blades that are mutually matched, cone-shaped bumps on the blades are in contact with seeds to break shells of the seeds, thereby the processing efficiency is increased to a great extent.In addition, because resonance is not easy to occur, the noise during operation is smaller.

The invention belongs to the field of seed treatment, and in particular relates to a surface sterilization technology of spruce seeds. The specific technical scheme is: use 20% to 30%, w / v, of H 2 o 2 Soak the seeds in the solution for 5-30 minutes. The sterilization operation is carried out by using a specific petri dish, and the petri dish includes at least two layers, one of which is a treatment layer that can hold the seeds to be sterilized, and the liquid circulation between each layer is controllable. The invention provides a surface sterilization method applicable to spruce seeds for the first time, and the sterilization effect is as high as 100%; and based on the method, a seed germination method is provided, and the highest germination rate is also as high as 100%. In order to sterilize more conveniently and accurately, the invention also provides a petri dish, which can carry out multi-gradient and multi-repeat sterilization tests at the same time, effectively improving the sterilization effect.