Method for AMF spore surface disinfection

A technique for arbuscular mycorrhizal fungi and surface disinfection, applied in the direction of disinfectants, botany equipment and methods, chemicals for biological control, etc., can solve the problems of immersion, poor disinfection, and difficulty, and achieve The operation is simple and gentle, and the effect of using less disinfectant

Inactive Publication Date: 2011-08-31
NORTHWEST A & F UNIV
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  • Abstract
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  • Claims
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Problems solved by technology

Because the spores of AMF have the characteristic of sticking to the wall, in general utensils, they tend to stick to the wall of the utensil, and it is difficult to make them completely submerged in the disinfectant solution, so they cannot be well disinfected

Method used

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  • Method for AMF spore surface disinfection
  • Method for AMF spore surface disinfection
  • Method for AMF spore surface disinfection

Examples

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Embodiment Construction

[0017] The present invention will be described in further detail below in conjunction with accompanying drawing:

[0018] a. Spare 10 sets of 60mm glass petri dishes, ordinary filter paper and absorbent paper with a diameter of 75mm, take two pieces of filter paper, fold their edges up and lay them on the bottom of the petri dish, leaving a gap between the folded up edge and the petri dish wall For transfer, cut some absorbent paper to fit the bottom of the petri dish, put 4 sheets into the petri dish, put 7 sets of petri dishes in total, and leave two sets of empty petri dishes for transferring double-layer filter paper, wrap the petri dish Strictly, spare 200ml of distilled water and 2 rubber-tip droppers, and sterilize the Petri dish, distilled water and rubber-tip droppers at 121°C for 20 minutes by high-pressure steam;

[0019] b. Prepare 20mL of spore surface disinfectant containing 50g / L chloramine T, 50mg / L chloramphenicol, 100mg / L gentamicin sulfate, 200mg / L streptomy...

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Abstract

The invention discloses a method for AMF spore surface disinfection, with the technical scheme comprising: putting filter paper and absorbent paper respectively inside several culture dishes; preparing spore surface antiseptic solution; putting the filter paper carrying AMF spores between the double-layer filter paper inside the culture dish; absorbing the antiseptic solution for dropping on the upper filer paper in a dropwise manner; shifting the double-layer filter paper inside the culture dish with filter paper for absorbing the adsorbed antiseptic solution via the filter paper; taking outthe upper filter paper and putting the upper filter paper inversely inside another culture dish, and transferring the AMF spores on the filter paper, thereby completing the surface disinfection. The invention realizes the surface disinfection of AMF spores through the combination of double-layer filter paper and culture dishes. The method is easy and mild to operate, consumes relatively low amount of antiseptic solution, and causes no loss of spores before or after disinfection, without affecting the vitality of the spores and without the assistance of other apparatus.

Description

technical field [0001] The invention relates to a surface disinfection method, in particular to a surface disinfection method for arbuscular mycorrhizal fungus spores, which belongs to the field of microorganism cultivation. Background technique [0002] Arbuscular mycorrhizal fungi (AMF) are a type of plant symbiotic bacteria that cannot be purely cultured. In the study of the non-symbiotic stage and physiology and biochemistry of AMF, it is often necessary to disinfect the surface of its spores to exclude the interference of other microorganisms. Because the spores of AMF have the characteristic of sticking to the wall, in general utensils, they tend to be attached to the wall of the utensil, and it is difficult to make it completely submerged in the disinfectant solution, so that it cannot be well sterilized. [0003] The most commonly used AMF spore surface disinfection methods are: "Kitasato beaker" method and "Eppendorf surface sterilized" method. The "Kitasato beaker...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L2/18A01N47/44A01P1/00A01N37/20A01N43/16A01N43/80A61L101/36A61L101/32A61L101/48
Inventor 唐明孙学广陈辉
Owner NORTHWEST A & F UNIV
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