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United preparation technology of earthworm elastin and lumbrukinase

A joint preparation and lumbrokinase technology, which is applied to the preparation method of peptides, enzymes, hydrolytic enzymes, etc., can solve the problems of low utilization and waste of earthworms, and achieve the effects of improving utilization, reducing production costs and improving quality

Active Publication Date: 2019-12-24
倪成 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the traditional preparation process can only extract a single product from earthworms, which will inevitably cause a certain waste and make the utilization of earthworms low.

Method used

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  • United preparation technology of earthworm elastin and lumbrukinase
  • United preparation technology of earthworm elastin and lumbrukinase
  • United preparation technology of earthworm elastin and lumbrukinase

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0056] a) Freeze etching

[0057] 1) Low-temperature quick-freezing: after evacuating the sundries in the abdominal cavity of earthworms, after cleaning, place them at -45°C for 4 hours to obtain frozen earthworms;

[0058] 2) Constant temperature thawing: transfer the frozen earthworms obtained in step 1) to thaw at 70°C, and collect the earthworm exudate;

[0059] 3) Repeat step 1), step 2) 7 times, collect and merge the earthworm exudate, and collect the remaining part of the earthworm;

[0060] b) Separation of elastin by countercurrent suspension method

[0061] The remaining material after the earthworm exudate is collected in step a) is subjected to countercurrent suspension and separation, and the temperature of the deionized water used is 4° C. to remove impurities to obtain earthworm elastin;

[0062] c) Extraction of earthworm elastic (collagen) protein

[0063] Add deionized water to the earthworm elastin obtained in step b), the mass ratio of earthworm elastin ...

Embodiment 2

[0070] a) Freeze etching

[0071] 1) Low-temperature quick-freezing: After emptying out the sundries in the abdominal cavity of earthworms, clean them and place them at -45°C for 8 hours to obtain frozen earthworms;

[0072] 2) Constant temperature thawing: transfer the frozen earthworms obtained in step 1) to thaw at 90°C, and collect the earthworm exudate;

[0073] 3) Repeat step 1), step 2) 10 times, collect and merge the earthworm exudate, and collect the remaining part of the earthworm;

[0074] b) Separation of elastin by countercurrent suspension method

[0075] The material remaining after the earthworm exudate is collected in step a) is subjected to countercurrent suspension and separation, and impurities are removed to obtain earthworm elastin;

[0076] c) Extraction of earthworm elastic (collagen) protein

[0077] Add deionized water to the earthworm elastin obtained in step b), keep it warm in a water bath at 90° C. for 2 hours, and then filter under vacuum nega...

Embodiment 3

[0084] a) Freeze etching

[0085] 1) Low-temperature quick-freezing: after evacuating the sundries in the abdominal cavity of earthworms, after cleaning, place them at a temperature of -45°C for 6 hours to obtain frozen earthworms;

[0086] 2) Constant temperature thawing: transfer the frozen earthworms obtained in step 1) to thaw at 75°C, and collect the earthworm exudate;

[0087] 3) Repeat step 1), step 2) 8 times, collect and merge the earthworm exudate, and collect the remaining part of the earthworm at the same time;

[0088] b) Separation of elastin by countercurrent suspension method

[0089] The material remaining after the earthworm exudate is collected in step a) is subjected to countercurrent suspension and separation, and impurities are removed to obtain earthworm elastin;

[0090] c) Extraction of earthworm elastic (collagen) protein

[0091] Add deionized water to the earthworm elastin obtained in step b), heat it in a water bath at 85° C. for 2 hours, and th...

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Abstract

The invention discloses a united preparation technology of earthworm elastic (collagen) protein and lumbrukinase. Earthworms are used as raw materials, by a freeze-etching method, a crust layer and anelastin layer are thoroughly separated, and collected earthworm exudate is used as pulp and can be used for producing the lumbrukinase; and the surplus parts are subjected to processing by a countercurrent flow suspension method for refining of elastic collagen protein, and then through the steps of glue cooking, decolorization, membrane separation, concentration, freeze drying and the like, theearthworm elastic collagen protein is obtained. According to the united preparation technology disclosed by the invention, the utilization degree of the earthworms is increased, no additive componentsare used in the process of producing the earthworm elastic collagen protein and the lumbrukinase, the quality of the earthworm elastic (collagen) protein and the lumbrukinase is increased, the production cost of the earthworm elastic (collagen) protein and the lumbrukinase is reduced, besides, the preparation technology is simple, the vitality of the lumbrukinase cannot be influenced, and industrialized production can be realized.

Description

technical field [0001] The invention relates to the field of earthworms, in particular to a joint preparation process of earthworm elastin (collagen) protein and lumbrokinase. Background technique [0002] Ancient medical classics contain that earthworms are cold in nature and non-toxic, and return to the liver, lung, and kidney meridians. It has the effects of expelling wind and relieving heat, diuretic and anti-inflammatory, and relieving asthma and activating collaterals; the traditional Chinese medicine prescription with earthworms as an adjuvant has been passed down in official documents and folklore, and has been used in ancient and modern times. Today, there are a large number of institutions and organizations dedicated to introducing the efficacy of earthworms into social productivity; around the research and development hotspots of earthworm medicine and health care, earthworm functional food, and earthworm protein feed, the scientific and technological category of ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/435C07K1/14C07K1/34C12N9/64
CPCC07K14/43504C12N9/6405
Inventor 倪成倪宝谦
Owner 倪成
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