Method for detecting surviving gene based on graphene-gold composite material electrochemical DNA (Deoxyribose Nucleic Acid) biosensor

A biosensor and composite material technology, applied in the field of detection of osteosarcoma-related gene survivin, can solve the problems of expensive, time-consuming, and low accuracy, and achieve the effects of improving specificity, enhancing sensitivity, and stabilizing the structure

Inactive Publication Date: 2013-04-24
FUJIAN MEDICAL UNIV
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[0006] At present, clinical genetic testing methods include flow cytometry, chromosome analysis, FISH, RT-

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  • Method for detecting surviving gene based on graphene-gold composite material electrochemical DNA (Deoxyribose Nucleic Acid) biosensor
  • Method for detecting surviving gene based on graphene-gold composite material electrochemical DNA (Deoxyribose Nucleic Acid) biosensor
  • Method for detecting surviving gene based on graphene-gold composite material electrochemical DNA (Deoxyribose Nucleic Acid) biosensor

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[0036] In order to make the technical problems, technical solutions and beneficial effects to be solved by the present invention clearer, the present invention will be further described in detail below in conjunction with the embodiments. It should be understood that the specific embodiments described here are only used to explain the present invention, not to limit the present invention.

[0037] The mass fraction of the following concentrated nitric acid is 65%, and the H in the commonly used concentrated sulfuric acid 2 SO 4 The mass fraction of is 98.3%.

[0038] The embodiment of the present invention is realized like this (see figure 1 ), a nano-electrochemical biosensor for detecting osteosarcoma-related gene survivin, the gene to be tested is survivin gene, comprising the following steps:

[0039] (1) Search the survivin gene sequence from the NCBI gene database, select one of them as a synthetic probe, the length of the probe is 30 bases, and synthesize the corresp...

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Abstract

The invention discloses a method for detecting a surviving gene based on a graphene-gold composite material electrochemical DNA (Deoxyribose Nucleic Acid) biosensor. The method comprises the following steps: a specific probe is designed according to a gene segment to be detected; a capture probe is self-assembled on the surface of G-3DAu/GCE through a gold-sulfur bond; the capture probe and a signal probe with the tail end marked by biotin are respectively combined with a target DNA to form a 'sandwich' model in the presence of the target DNA; a horse radish peroxidase marked by avidin can be combined with the biotin marked by the signal probe, so that the HRP (Horse Radish Peroxidase) can be fixed on the surface of an electrode; the electrode is placed in a base solution of 3, 3', 5, 5'-tetramethyl benzidine (TMB) and H2O2, and the H2O2 can oxidize TMB to generate a bidiazotizedbenzidine material under the catalyzing of the HRP, so that an electrochemical signal is generated. The method has the advantages of being simple, quick and green in preparation process, and high in selectivity and sensitivity.

Description

[0001] The invention relates to a method for detecting the osteosarcoma-related gene survivin based on the graphene / three-dimensional nano-gold nanocomposite material electrochemical DNA biosensor. Background technique [0002] In recent years, electrochemical DNA sensors have been widely considered as a promising detection method in the diagnosis of gene-related diseases and other biological analyzes due to their advantages of fast response, high sensitivity, easy operation, miniaturization, and low cost. . In order to further enhance the sensitivity and selectivity of sensors, nanostructured materials such as nanoparticles, nanoporous materials, and nanocarbon materials have been widely introduced into biosensors as transducers for enhanced sensing. [0003] The carbon atom arrangement of graphene is the same as the single atomic layer of graphite. It is a single-layer two-dimensional crystal composed of carbon atoms arranged in a honeycomb lattice with sp2 mixed orbitals. ...

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IPC IPC(8): G01N27/26G01N27/327
Inventor 林新华刘爱林钟光贤陈锦元翁少煌
Owner FUJIAN MEDICAL UNIV
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