Biological sample signal amplification method adopting combination of terahertz metamaterials and nanogold particles

A technology of nano-gold particles and biological samples, which is applied in the field of signal amplification of biological samples combined with terahertz metamaterials and nano-gold particles. problems, to meet the needs of rapid detection, high detection sensitivity, and improve the effect of detection sensitivity

Active Publication Date: 2015-10-14
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the disadvantages of low energy of terahertz wave source and limited sensitivity of direct detection, it is difficult to use this technology for rapid detection of trace samples.

Method used

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  • Biological sample signal amplification method adopting combination of terahertz metamaterials and nanogold particles
  • Biological sample signal amplification method adopting combination of terahertz metamaterials and nanogold particles
  • Biological sample signal amplification method adopting combination of terahertz metamaterials and nanogold particles

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] (1) Metamaterial cleaning;

[0048] Pick up a complete metamaterial with tweezers, wash it with deionized water, phosphate buffer (Sigma company), and deionized water three times, and dry it with nitrogen;

[0049] (2) Configuration of gold-labeled avidin;

[0050] Put on clean gloves, pipette 300 μL of ordinary avidin (pH about 7) with a concentration of 1 mg / mL into a clean centrifuge tube, and then pipette with a pipette gun with a concentration of 20 nmol / L Nano-gold solution (pH about 10) 0.5mL, mix the two (the ratio of ordinary avidin to nano-gold is about 2500:1), shake it on a shaker at room temperature for 15 minutes, and put it in the refrigerator Store in a medium temperature of 4°C, and the storage time is greater than or equal to 0.5 hours;

[0051] (3) Gold-labeled avidin extraction;

[0052]Take out the centrifuge tube containing the gold-labeled avidin from the refrigerator, take another centrifuge tube of the same type and fill it with the same amou...

Embodiment 2

[0068] (1) Metamaterial cleaning;

[0069] Pick up a complete metamaterial with tweezers, wash it with deionized water, phosphate buffer (Sigma company), and deionized water three times, and dry it with nitrogen;

[0070] (2) Configuration of gold-labeled avidin;

[0071] Put on clean gloves, pipette 300 μL of ordinary avidin (pH about 5) with a concentration of 0.8 mg / mL into a clean centrifuge tube, and then pipette with a pipette gun with a concentration of 20 nmol / L The nano-gold solution (pH is about 9) 0.5mL, the two are mixed (the ratio of the amount of ordinary avidin to nano-gold is about 2000:1), shaken on a shaker at room temperature for 15 minutes, and Store in the refrigerator, the storage temperature is 0°C, and the storage time is greater than or equal to 0.5 hours;

[0072] (3) Gold-labeled avidin extraction;

[0073] Take the gold-labeled avidin out of the refrigerator, take another centrifuge tube of the same type and inject the same amount of deionized wa...

Embodiment 3

[0091] (1) Metamaterial cleaning;

[0092] Pick up a complete metamaterial with tweezers, wash it with deionized water, phosphate buffer (Sigma company), and deionized water three times, and dry it with nitrogen;

[0093] (2) Escherichia coli antibody-coupled gold nanoparticles;

[0094] Put on clean gloves, pipette 2 μL of Escherichia coli antibody (pH about 9) with a concentration of 1 mg / mL into a clean centrifuge tube, and then pipette with a pipette gun with a concentration of 20 nmol / L nano Gold solution (pH about 8) 0.5mL, mix the two (the ratio of the amount of E. coli antibody to nano-gold substance is about 10:1), shake on a shaker at room temperature for 15 minutes;

[0095] (3) Extraction of Escherichia coli antibody-coupled gold nanoparticles;

[0096] Take the Escherichia coli antibody-conjugated gold nanoparticles out of the refrigerator, take another centrifuge tube of the same type and fill it with the same amount of deionized water, balance it, and centrifuge...

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Abstract

The invention discloses a biological sample signal amplification method adopting the combination of terahertz metamaterials and nanogold particles. The method comprises the following steps: preparing a plurality of biological sample solutions with different concentrations and gold standard avidin solutions with different concentrations; dropwise adding the biological sample solutions onto the surfaces of the terahertz metamaterials, and airing at the room temperature; dropwise adding the gold standard avidin solutions on the surfaces of the terahertz metamaterials, and airing at the room temperature; collecting terahertz time-domain signals of all sample points to be measured on the surfaces of the terahertz metamaterials and reference sample points; calculating the transmissivity or the reflectivity of all the sample points to be measured and the reference sample points according to the terahertz time-domain signals; and calculating to obtain the frequency shift of transmission peaks or reflection peaks according to the frequency value corresponding to the lowest point of the transmissivity or the reflectivity. According to the method, the terahertz metamaterials and the nanogold particles are used for modifying; the sample signals are amplified by using an electric field local enhancement effect of the metamaterials; the electric field distribution effect is changed by nanogold, and the sample signals are further amplified by nanogold modifying; and the detection is high in sensitivity, the operation is convenient and fast, and fast detection requirements increased day by day are met.

Description

technical field [0001] The invention relates to a method for amplifying terahertz signals of biological samples, in particular to a method for amplifying signals of biological samples in combination with terahertz metamaterials and gold nanoparticles. Background technique [0002] With the development of detection technology, spectral detection technology has gradually attracted the attention of scholars at home and abroad because of its rapid and simple detection. Terahertz spectroscopy, as an emerging spectroscopy technology, has gradually attracted people's attention. Since the vibration and rotational energy levels of many macromolecules fall in the terahertz band, terahertz waves are considered to be a very potential band for the detection of biological samples. For fields where terahertz spectroscopy has great application prospects, such as safety, biology, medicine, agriculture, and material characterization, there is a demand for trace or even ultra-trace nondestruc...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/3586G01N33/543
CPCG01N21/3586
Inventor 应义斌徐文道谢丽娟
Owner ZHEJIANG UNIV
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