124results about How to "Meet the needs of rapid detection" patented technology

The invention discloses a biological sample signal amplification method adopting the combination of terahertz metamaterials and nanogold particles. The method comprises the following steps: preparing a plurality of biological sample solutions with different concentrations and gold standard avidin solutions with different concentrations; dropwise adding the biological sample solutions onto the surfaces of the terahertz metamaterials, and airing at the room temperature; dropwise adding the gold standard avidin solutions on the surfaces of the terahertz metamaterials, and airing at the room temperature; collecting terahertz time-domain signals of all sample points to be measured on the surfaces of the terahertz metamaterials and reference sample points; calculating the transmissivity or the reflectivity of all the sample points to be measured and the reference sample points according to the terahertz time-domain signals; and calculating to obtain the frequency shift of transmission peaks or reflection peaks according to the frequency value corresponding to the lowest point of the transmissivity or the reflectivity. According to the method, the terahertz metamaterials and the nanogold particles are used for modifying; the sample signals are amplified by using an electric field local enhancement effect of the metamaterials; the electric field distribution effect is changed by nanogold, and the sample signals are further amplified by nanogold modifying; and the detection is high in sensitivity, the operation is convenient and fast, and fast detection requirements increased day by day are met.

The invention relates to a multiplex PCR (polymerase chain reaction) primer, a probe and a gene chip for detecting the bluetongue virus, foot and mouth disease virus and bovine viral diarrhea virus. The multiplex PCR primer and probe have the nucleotide sequences shown by SEQ ID No.1 to SEQ ID and No.9. The gene chip comprises a solid-phase carrier, a sample application quality control probe, a positive hybrid quality control probe and a multiplex PCR primer for detecting the bluetongue virus, foot and mouth disease virus and bovine viral diarrhea virus and the corresponding probe. In the invention, the forward primers of three viruses are marked with fluorescence, a gene chip detection technology carrying three viruses in animal fur is established based on multiplex RT-PCR (reverse transcription-polymerase chain reaction), and the RNA virus in the fur can be sensitively and specifically detected with high flux; the three viruses are screened at the same time in detection once, and the situation that a specific method is required for each virus before is changed, thereby saving the diagnosis time, meeting the needs for quick detection of mass imported/exported fur samples of the exit-entry inspection and quarantine departments and the fur import and export enterprises, and realizing relatively high application values.

The invention discloses a real-time fluorescence quantitative PCR(Polymerase Chain Reaction) detection kit and related primers for thalassemia genes. The kit comprises more than one pair of the following primers: SEQ ID NO.1 and SEQ ID NO.2 aiming at TATA kits nt-28 (A->G) (-28M), -29 (A->G) (-29M) and CAP-40-43 (AACC) (CAPM) genotypes; SEQ ID NO.3 and SEQ ID NO.4 aiming at CD14-15 (+G) (14-15M), CD17 (A->T) (17M) and the initiation codon mutant ATG->AGG(IntM) genotypes; SEQ ID NO.5 and SEQ ID NO.6 aiming at CD26 (G->A)(betaEM) CDs27-28+C(27-28M), IVS1-1(G->T)(IVS1-1M), IVS1-5(G->C)(IVS1-5M) genotypes; SEQ ID NO.7 and SEQ ID NO.8 aiming at CDs41-42-TCTT (41-42M), CD43G->T(43M), 41-42M/41-42M genotypes; SEQ ID NO.9 and SEQ ID NO.10 aiming at CD71-72(+A) (71-72M) genotypes; SEQ ID NO.11 and SEQ ID NO.12 aiming at IVS2-654C->T(654M) and 654M/654M genotypes; and SEQ ID NO.13 and SEQ ID NO.14 aiming at constant spring (CS), Quong Sze (QS) and Westmead (WS) genotypes. The kit has the advantages of good detection specificity and high detection sensitivity.

The invention discloses a molecularly-imprinted polymer-graphene composite material modified electrode. The molecularly-imprinted polymer-graphene composite material modified electrode comprises a molecularly-imprinted polymer-graphene composite material and a glassy carbon electrode, wherein the glassy carbon electrode is used as a base body; the molecularly-imprinted polymer-graphene composite material is modified on the glassy carbon electrode. The invention further provides a preparation method of the molecularly-imprinted polymer-graphene composite material modified electrode and an application of the molecularly-imprinted polymer-graphene composite material modified electrode to detection of aniline and 4,4'-methylenedianiline (MDA). The molecularly-imprinted polymer-graphene composite material modified electrode has the advantages of high responding speed, good selectivity, high sensitivity and the like and is suitable for rapidly detecting the aniline and the MDA in food safety and environmental sanitation.

The invention relates to a tunnel section measurement device and a measurement method thereof. The measurement device comprises a measurement mechanism and a shooting mechanism, wherein the measurement mechanism comprises a moving component, as well as a rotating component, ranging devices, a transmission device, a control device and a power supply device which are arranged on the moving component; the rotating component comprises a displacement rotary table arranged on the measurement mechanism and a rotating shaft controlled by the displacement rotary table; the end part of the rotating shaft is provided with a mounting seat and extends out of the measurement mechanism; a plurality of ranging devices are arranged on the mounting seat according to a set distribution state, and can generate ranging light spots during ranging; the transmission device is used for generating arc profile light rays on the inner wall of a tunnel; and the shooting mechanism comprises a tripod and a camera arranged on the tripod, and is arranged at a certain distance to the measurement mechanism. Compared with the prior art, the tunnel section measurement device has the characteristics of relatively highprecision, relatively high speed, low cost, convenience in operation and the like, and can meet the requirement of quickly detecting a tunnel under the condition of ensuring certain precision.

The invention discloses a method for rapidly detecting biogenic amine by thin layer chromatography in one step and belongs to the technical field of food analysis and safety. The method disclosed by the invention comprises the following steps: (1) preparing an activated silica gel; (2) preparing an activated thin layer chromatography plate; and (3) analyzing the thin layer chromatography in the biogenic amine in a food sample. The method for rapidly detecting biogenic amine by thin layer chromatography in one step, disclosed by the invention, can be used for rapidly measuring the content of biogenic amine in products such as aquatic products, meat, diary products, pickled vegetables, spirits and esters. Compared with other analytical methods, according to the method, a derivating agent is directly fixed on a medium during activation, and the extracted and purified sample needs not to be derived, is diluted to a calibrated range and can be directly dispensed to be expanded for measurement; moreover, no expensive analytical instruments are required, so that the method is low in expense and simple and rapid to operate, and can effectively lower the monitoring cost in food production and circulation.

The invention relates to a ceramic tile flatness on-line detection method including a device and a detection method. The device includes a detection support fixed on a conveyor belt framework, non-contact distance measuring sensors, and a computer. A detection plane in parallel with a conveyor belt is arranged on the detection support. Five sensors are fixed on the detection plane and face the conveyor belt. The sensors synchronously collect distance data from conveyed ceramic tiles. The computer connected to the sensors calculates slopes and compares the slopes with a standard slope so as to judge whether the ceramic tiles are qualified or not. A photoelectric switch is arranged on the conveyor belt framework. A photoelectric switch signal controls the collection action of the sensors after being sent to the computer. The non-contact distance measuring sensors make the conveyor belt nonstop on-line detection possible. The on-line detection efficiency is improved, and vibration errors will not be caused by frequent pause and starting, and the fast detection demand of a production line can be satisfied.

The invention provides a method for determining antibiotic in an environment water body through synchronization of liquid chromatography and mass spectrometry. The method comprises the following steps: 1) reducing background interference from an experiment vessel; 2) preparing a sample; 3) stably preserving the sample; 4) performing solid-phase extraction and enrichment on a to-be-measured objectin a water phase; 5) purifying the sample and eluting the sample; 6) performing concentration and enrichment; and 7) determining the material by using the high-performance liquid chromatography-tandemmass spectrometry. The method absorbs good enrichment capability of solid-phase extraction and good separating capability of a liquid phase, and characteristic of accurate quantification of added standard internal standard substances, and the method has obvious effect for detecting the trace-quantity antibiotic in different types of the environment water bodies. The method performs exploration and test on a solid-phase extraction elution solvent, a liquid-phase separating mobile phase, and mass spectrum conditions.

The invention provides a ratio type fluorescent molecularly imprinted paper chip, a preparation method and application thereof and belongs to the technical field of material science and engineering and micro-fluidic chips. The method comprises the following steps that: 1, the surface of cellulose paper is sequentially modified with CDs and an APTES-NBD conjugate fluorescent material; difenoconazole imprinted holes are formed in the surface of the cellulose paper through a surface imprinting technology, so that fluorescent molecularly imprinted paper can be prepared; and the fluorescent molecularly imprinted paper is fixed on a microfluidic substrate of a three-dimensional structure, so that the ratio type fluorescent molecularly imprinted paper chip can be obtained. The microfluidic rapiddetection paper chip of the present invention can realize the rapid detection of difenoconazole and has the advantages of low toxicity, portability, high efficiency, economical efficiency and the like.

The invention provides an OTA (Ochratoxin A) chemiluminescence detection method based on a label-free aptasensor. According to the technical scheme, the OTA chemiluminescence detection method comprises: fixing an aptamer capturing probe on the surface of a magnetic microsphere; combining the capturing probe and OTA with a competition relationship of an OTA aptamer respectively; obtaining a quantitative relationship between the quantity of magnetic microsphere surface detection probes and the concentration of OTA to be detected based on the fact that the specific combining capability of the aptamer is stronger than the binding force of complementary strands; finally, carrying out chemiluminescence detection on the aptasensor. A chemiluminescence detection mechanism of the OTA chemiluminescence detection method is realized based on a principle that a guanine base (G) on an OTA aptamer strand and a chemiluminescence reagent phenylglyoxal (PG) are subjected to an instantaneous derivative reaction to generate chemiluminescence, so that a labeling link of other chemiluminescence markers is omitted; operation steps are simplified on the basis of guanrateeing the sensitivity and the detection time is shortened; a chemiluminescence anslysis method has the advantages of wide linear range, simple equipment and easiness of operation, so that full-automatic detection is hopeful to realize.

The invention discloses a low-speed denial of service (LDoS) attack detection method based on multi-flow characteristics and an Adaboost (MF-Ada) algorithm, and belongs to the field of network security. The method comprises the following steps: in unit time, capturing all related data messages in a network key routing node to form a training sample and a test sample; performing feature extractionand feature selection on the training sample and the test sample to obtain optimal feature data of the training sample and optimal feature data of the test sample; training an Adaboost classificationmodel by using the optimal feature data of the training sample, so that the Adaboost classification model learns and memorizes the features of the LDoS attack to obtain a model capable of being used for LDoS attack detection; and detecting the optimal feature data of the test sample by using the trained Adaboost classification model; and judging whether the LDoS attack occurs in the unit time corresponding to the optimal feature data or not according to a judgment criterion. The detection method based on the MF-Ada algorithm provided by the invention has the advantages of relatively low falsealarm rate and missing report rate and self-adaptive parameter adjustment, and is an LDoS attack detection method with relatively good detection performance.

The invention discloses a pressure sensor detection system and a pressure sensor detection method. The system is characterized by comprising a master control chip, wherein the master control chip is provided with a first reference input end, a detection input end and a second reference input end; the first reference input end is connected with a first reference pressure sensor; a pressure detection port of the first reference pressure sensor is communicated with the atmosphere; the second reference input end is connected with a second reference pressure sensor; a pressure detection port of the second reference pressure sensor is communicated with a negative pressure source; and the master control chip is connected with an LCD display. The pressure sensor detection system has the advantages of simple structure, low cost, and capacities of finishing accurate detection of static flow and dynamic flow and also meeting the requirements on rapid detection in motorcycle industrialization.

The invention discloses a method for detecting sulfur dioxide in food, which comprises the following steps: 1) simply pretreating samples; 2) starting up, entering a main detection interface, clicking on a food quality analysis module and then entering a 'quantitative detection' interface, setting a 'detection item' as sulfur dioxide, and setting a 'product type' according to the actual product type; 3) operating according to the steps of sulfur dioxide detection instructions on the 'quantitative detection' interface of a instrument software; and 4) directly reading detection results. The invention is characterized in that: detecting personnel only need to simply pretreat the samples, and then carry out blank correction and sample detection so as to quickly obtain higher-accuracy quantitative analysis results of the index of sulfur dioxide in food. The method has simple operation, rapid detection speed, accurate detection results and high detection efficiency.

The invention provides a method for identifying a nitrogen-containing compound in kaoliang spirit, and belongs to the technical field of analysis and detection. The method comprises the following steps: extracting the nitrogen-containing compound in the kaoliang spirit; detecting and analyzing the obtained nitrogen-containing compound in the kaoliang spirit by using a gas chromatography-nitrogen phosphorus detector-mass spectrometry coupled technology; qualitatively and quantitatively analyzing the nitrogen-containing compound. The nitrogen-containing compound in a kaoliang spirit sample is analyzed by headspace solid-phase microextraction and the gas chromatography-nitrogen phosphorus detector-mass spectrometry coupled technology, and the qualitative and quantitative analysis method for the nitrogen-containing compound in the kaoliang spirit is formed. The method is simple to operate, feasible, and wide in applicability.

The invention discloses a high-throughput micro-fluidic LAMP chip for detecting livestock respiratory tract pathogens and a detection method, and belongs to the technical field of biochips. The LAMP chip comprises an LAMP amplification reagent, a DNA template, an LAMP quality control positive control, an LAMP quality control negative control, a detection chip, a chip probe and a microfluidic LAMPchip analyzer, wherein the detection chip is arranged in the isothermal amplification microfluidic LAMP chip analyzer; and the chip probe comprises nucleotide sequences as shown in SEQ ID NO: 1-23. The LAMP chip is used for detecting specific nucleic acid fragments of mannheimia haemolytica, pasteurella multocida, mycoplasma, arcanobacterium pyogenes and klebsiella pneumoniae. The LAMP chip provided by the invention can complete detection within 50 minutes, has a detection limit of 1.5x10<-2> ng/mu L, can monitor main livestock respiratory tract pathogens in real time, has the characteristicsof simple operation, rapid detection, high sensitivity and strong specificity, and brings convenience to detection of the livestock respiratory tract pathogens.

The invention discloses a TiO2 (titanium dioxide) nanowire biosensor chip and system for fast detecting Enteropathogenetic Escherichia coli (EPEC). The TiO2 nanowire biosensor chip is that TiO2 nanowires are connected between two parallel metal electrodes; the TiO2 nanowires are in a coaxial layered nano-structure; and the surface of the TiO2 nanowires is bonded with hydroxyl. A sample feeding container and a sample discharging container of the biosensor of a system for fast detecting EPEC are communicated by a microfluidic channel; the TiO2 nanowires in the TiO2 nanowire biosensor chip are disposed in the microfluidic channel; the two parallel metal electrodes in the TiO2 nanowire biosensor chip are respectively disposed at two sides of the microfluidic channel; and the TiO2 nanowires are disposed in an alternating magnetic field; a first peristaltic pump of the system is communicated with the sample feeding container by a bacteria feeding tube; a second peristaltic pump is communicated with the sample feeding container by a cleaning solution feeding tube; and an impedance detection circuit is respectively and electrically connected to the two metal electrodes, the first peristaltic pump and the second peristaltic pump. In the invention, complicated pre-processes for bacteria samples are not needed; and the bacteria detection time is shortened.

The invention aims at providing a Photobacterium leiognathi YL bacterial strain deriving from marine environment and quantitative detection on environmental pollutants by using the bacterial strain. In the invention, the luminous bacterial YL deriving from the marine environment is obtained by separating and purifying, is Gram-negative bacilli, and is rhabditiform. The luminous bacterial YL is identified to be Photobacterium leiognathi according to a phylogenetic tree established by a 16SrDNA sequencing result and a physiology and biochemistry result. The Photobacterium leiognathi YL is preserved in China Center for Type Culture Collection (CCTCC in short) with a collection number of M 206139. The 16SrDNA gene sequence of the luminous bacterial is submitted to GenBank nucleotide sequence database with the accession number of EF017227. The bacterial strain has the characteristics of rapid growth, stable luminous property, low requirements for nutrients, and sensibility to the environmental pollutants, and the like; the luminous intensity thereof is confirmed to form a maximum peak at 474 nm by fluorescence scanning; and the luminous intensity inhibition ratio thereof is in direct ratio to the concentration of multiple tested environmental pollutants, thereby the quantitative detection of the tested environmental pollutants can be realized. The invention has the characteristics of low cost, simple and convenient operation, high sensitivity and the like on detecting the environmental pollutants, , thereby satisfying the requirements on rapid detection of the pollutants.

The invention provides a method for identifying nitrogen-containing compounds in Moutai, which belongs to the technical field of analysis and detection, and includes a step of extracting nitrogen-containing compounds in Moutai; a method for detecting and analyzing the nitrogen-containing compounds in Moutai; The steps of qualitative and quantitative analysis of the nitrogen-containing compounds in the above obtained Moutai liquor. The present invention uses headspace solid-phase microextraction combined with gas chromatography-nitrogen and phosphorus detector-mass spectrometry to analyze nitrogen-containing compounds in Moutai liquor samples, forming a set of qualitative and quantitative analysis methods for nitrogen-containing compounds in Moutai liquor. Simple, easy to implement, and widely applicable.