Method for determining antibiotic in environment water body through synchronization of liquid chromatography and mass spectrometry

Abstract

The invention provides a method for determining antibiotic in an environment water body through synchronization of liquid chromatography and mass spectrometry. The method comprises the following steps: 1) reducing background interference from an experiment vessel; 2) preparing a sample; 3) stably preserving the sample; 4) performing solid-phase extraction and enrichment on a to-be-measured objectin a water phase; 5) purifying the sample and eluting the sample; 6) performing concentration and enrichment; and 7) determining the material by using the high-performance liquid chromatography-tandemmass spectrometry. The method absorbs good enrichment capability of solid-phase extraction and good separating capability of a liquid phase, and characteristic of accurate quantification of added standard internal standard substances, and the method has obvious effect for detecting the trace-quantity antibiotic in different types of the environment water bodies. The method performs exploration and test on a solid-phase extraction elution solvent, a liquid-phase separating mobile phase, and mass spectrum conditions.

Description

technical field [0001] The present invention relates to an antibiotic detection method, in particular to a method for synchronous determination of antibiotics in environmental water by high-performance liquid chromatography mass spectrometry. The method can realize efficient and accurate detection of trace antibiotics in different types of environmental water, and especially provides A kind of sample pretreatment by solid phase extraction, based on high performance liquid chromatography tandem mass spectrometry (LC-MS / MS), simultaneously detects macrolides, sulfonamides, trimethoprim, quinolones, and tetracyclines in environmental water Methods of 17 antibiotics in 5 categories. Background technique [0002] Antibiotics are widely used as effective drugs to treat various diseases. In China, the annual consumption of antibiotics is about 210,000 tons, and more than 80% of antibiotics are transported to the environment in their original form through industrial wastewater, med...

AI Technical Summary

Problems solved by technology

However, as more and more types of antibiotics are discharged into the water environment in recent years, they only target certain types or a certain type of compounds, which is not suitable for the analysis and detection of complex matrices.

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