Fluorescent immunochromatographic detection kit for detecting clostridium perfringens and application thereof

Abstract

The invention relates to a fluorescent immunochromatographic detection kit for detecting clostridium perfringens and application thereof. The fluorescent immunochromatographic detection kit comprises a fluorescent immunochromatographic detection device and a diluted sample solution, wherein the fluorescent immunochromatographic detection device comprises a test strip consisting of a bottom plate, a sample pad, a reagent pad, a nitrocellulose membrane and an absorptive pad; the sample pad, the reagent pad, the nitrocellulose membrane and the absorptive pad are sequentially fixed to the bottom plate in a staggered way; the reagent pad envelopes fluorescent microspheres marked by rabbit anti-clostridium perfringens IgG; a detection line on the nitrocellulose membrane envelops the rabbit anti-clostridium perfringens IgG; a quality control line envelops goat anti-rabbit IgG; the diluted sample solution is a nano silve-containing solution. The fluorescent immunochromatographic detection kit has the sensitivity of 10 pg / L and has the linear range of 40-1000 pg / L; the detection time is short; a special instrument and a professional technician are not required; the fluorescent immunochromatographic detection kit can meet needs for rapidly detecting the clostridium perfringens in hospitals, customs, health and quarantine departments and other units.

Description

technical field [0001] The invention belongs to the field of immunization applications, and relates to a technique for diagnosing or detecting Clostridium perfringens in medical institutions, epidemic prevention and quarantine departments and the like, and in particular to a fluorescent immunochromatographic detection kit for Clostridium perfringens and its application. Background technique [0002] Clostridium perfringens (C. perfringens), formerly known as Clostridium welchii or Clostridium perfringens, is the most common Clostridium among gas gangrene pathogens clinically, because it can decompose muscle and connective tissue Sugar produces a large amount of gas, leading to severe emphysema and large-scale tissue necrosis. In addition, this bacteria can form capsules in the body, so it is called Clostridium aerogenes. It is the main pathogenic bacteria of human gas gangrene. In 1892, American pathologist W.H. Welch et al. isolated this bacterium from a corpse, so it is al...

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Problems solved by technology

At present, this method is widely used in the primary screening of samples, but the disadvantage is that the detection sensitivity is low and it is difficult to carry out quantitative detection

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