Microfluidic chip, system and method integrating nucleic acid extraction, amplification and detection

Abstract

The invention relates to a microfluidic chip, a system and a method integrating nucleic acid extraction, amplification and detection. The chip comprises a sample inlet, a washing liquid storage cavity, an eluent storage cavity, a nucleic acid extraction cavity, a washing waste liquid receiving part and a constant temperature amplification cavity, wherein the nucleic acid extraction cavity is provided with nucleic acid extraction filter paper; the washing liquid storage cavity and the eluent storage cavity are respectively connected with the nucleic acid extraction cavity through one-way valves; the nucleic acid extraction cavity is connected with a washing waste liquid receiving part and a constant temperature amplification cavity; a washing liquid is pre-stored in the washing liquid storage cavity; an eluent is pre-stored in the eluent storage cavity; the washing liquid storage cavity is used for enabling the washing liquid to break through the one-way valve and flow through the nucleic acid extraction cavity for washing under driving of external force, and waste liquid flows into the washing waste liquid receiving part; and the eluent storage cavity is used for enabling the eluent to break through the one-way valve and enter the nucleic acid extraction cavity for elution under driving of external force, wherein the eluted nucleic acid solution flows into the constant-temperature amplification cavity, and waste liquid flows into an elution waste liquid cavity. The chip can rapidly, simply, stably and reliably complete nucleic acid extraction, amplification and detection.

Description

technical field [0001] The invention relates to the technical field of molecular diagnosis, in particular to a microfluidic chip, system and method integrating nucleic acid extraction, amplification and detection. Background technique [0002] Microfluidic chips are also known as Lab-on-a-Chip (LOC). Since the introduction of Micro-Electro-Mechanical System (MEMS) in the 1990s, related research and technologies have risen rapidly. , has received close attention from the scientific community. Microfluidic chip technology is an experimental technology for precise control of microfluidics in microchannels. Because of its advantages of low consumption, low cost, high throughput, and automatic operation, it is widely used in the field of biomedicine. One of the important The application is molecular diagnostic technology based on microfluidic chips. [0003] Nucleic acid is a molecular biomarker widely used in molecular diagnosis, food safety control and environmental monitorin...

AI Technical Summary

Problems solved by technology

However, like the conventional nucleic acid detection technology, the existing detection devices using this technology cannot get rid of the shortcomings of time-consuming sample preparation and cumbersome operation, and do not meet the needs of rapid and low-cost clinical diagnosis

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