Detection of environmental pollutants by using Photobacterium leiognathi YL bacterial strain

Abstract

The invention aims at providing a Photobacterium leiognathi YL bacterial strain deriving from marine environment and quantitative detection on environmental pollutants by using the bacterial strain. In the invention, the luminous bacterial YL deriving from the marine environment is obtained by separating and purifying, is Gram-negative bacilli, and is rhabditiform. The luminous bacterial YL is identified to be Photobacterium leiognathi according to a phylogenetic tree established by a 16SrDNA sequencing result and a physiology and biochemistry result. The Photobacterium leiognathi YL is preserved in China Center for Type Culture Collection (CCTCC in short) with a collection number of M 206139. The 16SrDNA gene sequence of the luminous bacterial is submitted to GenBank nucleotide sequence database with the accession number of EF017227. The bacterial strain has the characteristics of rapid growth, stable luminous property, low requirements for nutrients, and sensibility to the environmental pollutants, and the like; the luminous intensity thereof is confirmed to form a maximum peak at 474 nm by fluorescence scanning; and the luminous intensity inhibition ratio thereof is in direct ratio to the concentration of multiple tested environmental pollutants, thereby the quantitative detection of the tested environmental pollutants can be realized. The invention has the characteristics of low cost, simple and convenient operation, high sensitivity and the like on detecting the environmental pollutants, , thereby satisfying the requirements on rapid detection of the pollutants.

Description

technical field

[0001] The invention relates to a microorganism, in particular to a marine luminescent bacterium Photobacterium leiognathi YL strain which can be used for the detection of environmental pollutants. Background technique

[0002] According to the inventor's information and literature search, there are only three known genera of marine luminescent bacteria, which are Vibrio, Photobacterium, and Shewanella. On land, there are different short bacillus (Xenorhabdus). In an aerobic natural environment, luminescent bacteria can produce luciferase (LE), catalyze flavin mononucleotide (FMNH 2 ) and long-chain aliphatic aldehydes undergo oxidation reactions to emit blue-green fluorescence.

[0003] At present, Photobacterium phosphoreum is widely used in our country. There are some reports on the use of Photobacterium phosphoreum to detect environmental pollutants. Except for Luminescent bacteria, there is no report on using other species of luminescent bacteria to d...

Images