21 results about "Photobacterium phosphoreum" patented technology

The invention relates to the technical field of microorganisms, in particular to an antibacterial bacillus subtilis subsp. Deserticola strain and application thereof.Bacillus subtilis subsp. Deserticola D7-HSJ9 is obtained from mangrove forest rhizosphere soil by the applicant, and it is detected that the strain has good salt resistance, alkali resistance and antibacterial characteristics, and the bacillus subtilis subsp. Deserticola D7-HSJ9 can be used as a bacillus subtilis subsp. Deserticola strain. According to the present invention, the bacterial strain has characteristics of good antibacterial effect, particularly good antibacterial effect on streptococcus iniae, and good antibacterial effect on a plurality of pathogenic bacteria such as photobacterium dama subspecies, aeromonas salmonicida, staphylococcus aureus and the like, such that the bacterial strain can effectively replace the antibiotic so as to improve the prevention and control of fish diseases, and a good germplasm resource is provided for later preparation of microecological preparation products.

The invention relates to a varied massinia, and in particular relates to a separated varied massinia P2-4 with broad-spectrum phagocytosis activity on pathogenic bacteria, and the varied massinia P2-4 is used for phagocytosis in a bacterium cracking manner, so that the activity of the varied massinia P2-4 is improved. The compound has phagocytosis activity on pathogenic bacteria such as pseudomonas aeruginosa, shewanella alginosa, aeromonas cainea, aeromonas hydrophila and photobacterium damsela; the Marsinia variabilis P2-4 provided by the invention can be applied to inhibition of pathogenic bacteria in an aquaculture water body; by adding the varied maleinia sp. P2-4 or the microbial inoculum thereof into the aquaculture feed, the immunity and / or the oxidation resistance and / or the disease infection resistance of the organism of aquaculture animals can be enhanced; the varied marssonia sp. P2-4 disclosed by the invention can be used as a feed additive for aquaculture or a biocontrol product for aquaculture, and can also be used for preventing and / or treating diseases of aquaculture animals.

The invention relates to an alkali-resistant massinia sp., in particular to a separated alkali-resistant massinia sp. P2-6 with broad-spectrum phagocytosis activity on pathogenic bacteria, and the alkali-resistant massinia sp. P2-6 is used for phagocytosis in a bacterium splitting manner, and can be used for preparing the alkali-resistant massinia sp. P2-6 with broad-spectrum phagocytosis activity on pathogenic bacteria. The compound has phagocytosis activity on pathogenic bacteria such as aeromonas veronii, aeromonas hydrophila, aeromonas cainea, pseudomonas aeruginosa, shewanella alginate or photobacterium damsela and the like; the alkali-resistant Marsinia sp. P2-6 disclosed by the invention can be applied to inhibition of pathogenic bacteria in an aquaculture water body; alkali-resisting marssonia sp. P2-6 or a microbial agent thereof is added into an aquaculture feed, so that the organism immunity and / or oxidation resistance and / or disease infection resistance of aquaculture animals can be enhanced; the alkali-resistant Marsinia sp. P2-6 provided by the invention can be used as a feed additive for aquaculture or a biocontrol product for aquaculture, and can also be used for preventing and / or treating diseases of aquaculture animals.

The invention discloses bacillus velezensis Bve1910, which is preserved in the China Center for Type Culture Collection (CCTCC) and has the preservation number of CCTCC NO. M 20221965. The strain and metabolites thereof have strong antagonistic effects on more than ten types of marine culture animal pathogenic bacteria such as vibrio harveyi, vibrio erwiniae, vibrio canbayi, vibrio parahaemolyticus, vibrio alginolyticus, vibrio anguillarum, vibrio cholerae, vibrio shewanii, vibrio rotifer, pseudoalteromonas and photobacterium damarae, are strong in antibacterial activity and wide in antibacterial spectrum, and can be used for preparing marine culture animal pathogenic bacteria. According to the preparation method, the traditional Chinese medicine composition is simple in preparation method and rich and stable in metabolite, an ammonium sulfate precipitation extract, a hydrochloric acid precipitation methanol extraction extract, an ethyl acetate extraction extract and a chloroform extraction extract all have a broad-spectrum bacteriostatic effect, functional substances playing the broad-spectrum antagonistic bacteriostatic effect are compounded and diversified, and the directional selection effect on bacteria in the environment and ecology is not generated; and pathogenic bacteria are not easy to generate tolerance, so that the method has good application safety and ecological safety properties.

The invention belongs to the field of plant diseases, and particularly relates to a luminous labeled recombinant bacterium Xap-LUX and a construction method and application thereof. According to the present invention, a luxCDABE operon of a photobacterium phorbia luminescens is controlled by a constitutive promoter, and the luxCDABE operon is inserted into a chromosome of a peach bacterial perforation bacterium Xanthomonas arbocola pv.pruni so as to construct the stably expressed constitutive luminescence labeled recombinant bacterium Xap-LUX; the number of the bacteria can be evaluated conveniently, quickly and reliably based on the bioluminescence value of the bacteria. Therefore, the bioluminescence values of the luminescent labeled recombinant bacteria Xap-LUX in different medicaments are further quantitatively determined on a large scale, the antibacterial effects of different medicaments are evaluated, and the application of the strain and the reliability of the determination method are proved by using a conventional method.

The invention belongs to the technical field of microbiological detection, and particularly relates to a detection nucleotide sequence of photobacterium damsela subspecies and application of the detection nucleotide sequence. Aiming at the ureC gene of the photobacterium damsela subspecies, the invention designs a nucleotide sequence for fluorescent quantitative PCR detection, the detection sensitivity to a target gene in detection application is 7.10 copies / reaction, and the nucleotide sequence has the advantages of specificity, rapidness and stable result.

The present invention belongs to the technical field of bioengineering. Disclosed are Photobacterium rosenbergii and a use thereof in the preparation of a carrageenan oligosaccharide. The Photobacterium rosenbergii is specifically Photobacterium rosenbergii GDSX-4 with a 16S rDNA sequence as set forth in SEQ ID NO: 1. The Photobacterium rosenbergii GDSX-4 provided by the present invention can degrade κ-carrageenan to obtain a κ-carrageenan oligosaccharide. A crude enzyme liquid is prepared by means of the Photobacterium rosenbergii GDSX-4, and a reaction is carried out in a substrate solution containing κ-carrageenan at 40°C for 0.5-72 hours to prepare κ-neocarratetraose, κ-neocarrahexaose, and κ-neocarrabiose. The present invention provides a method for preparing a carrageenan oligosaccharide, wherein the reaction is mild and the product is uniform.

The invention belongs to the technical field of aquatic product pathogen gene editing, and particularly relates to a target gene knockout method for photobacterium damsela subsp. Piscicida (PDP), which is characterized in that the target gene knockout method for the photobacterium damsela subsp. Piscicida (PDP) is disclosed. The method comprises the following steps: obtaining a DNA fusion fragment with deletion of a target region fragment of a target gene (MshL) by adopting overlapping PCR (Polymerase Chain Reaction), constructing a recombinant vector by using the obtained DNA fusion fragment, and obtaining the target gene knocked-out photobacterium damnieri subspecies fish by taking escherichia coli S17-1 lambda pir as a donor bacterium of a recombinant plasmid and PDP as a recipient bacterium. Specific knockout of the PDP specific gene is achieved for the first time, the knockout efficiency is high (the single-exchange positive rate is 29.2%, and the double-exchange positive rate is 30.8%), the cost is low, and the method is suitable for construction of a PDP specific gene deleted strain and functional research of the specific gene.

The present invention belongs to the technical field of aquatic pathogen diagnosis, and specifically relates to a multiplex fluorescence quantitative PCR detection kit for common pathogens in aquaculture, and its application and detection method. The kit contains primers and probes for detecting common pathogens in aquaculture, and the 5' end of each probe is modified with a corresponding fluorescent marker; wherein the common pathogens are Edwardsiella piscicida (Ep), Photobacterium mermanii (Pd), Vibrio rotifer (Vr) and Pseudomonas fluorescens (Pf). The method realizes the simultaneous identification and quantitative detection of Ep, Pd, Vr and Pf, has high sensitivity and good specificity, can greatly improve the detection efficiency, save the detection cost, and is suitable for large-scale rapid detection of clinical samples.

The invention provides a preparation method and application of a pomegranate rind extract, and the preparation method comprises the following steps: crushing pomegranate rind by a crusher, sieving, soaking in double distilled water, boiling with big fire, boiling with small fire for 20-60 minutes, and concentrating with big fire to obtain the pomegranate rind extract. Compared with the prior art, the pomegranate peel single Chinese herbal medicine aqueous extract prepared by the invention has a remarkable inhibition effect on apostichopus japonicus skin ulcer pathogenic bacteria photobacterium damsonii, is relatively low in toxicity, and can be used for preventing and controlling apostichopus japonicus skin ulcer syndrome caused by the photobacterium damsonii. Moreover, a proper amount of pomegranate peel aqueous extract is mixed with bait to feed the apostichopus japonicus, so that the capability of resisting photobacterium damsonii subspecies infection of the apostichopus japonicus can be remarkably enhanced, and the survival rate of the apostichopus japonicus is increased.

The invention provides a genetically engineered bacterium as well as a preparation method and application thereof. The genetically engineered bacterium contains an orfZ gene which is introduced from an external source; the genetically engineered bacterium is selected from photobacterium LN01. When the genetically engineered bacterium is fermented by taking cane sugar and gamma-butyrolactone as precursors, the P3HB4HB can be efficiently synthesized, and the content of 4HB in the P3HB4HB is high. The genetically engineered bacterium is expected to be applied to industrial production of bio-based plastics, efficient production of P3HB4HB with high content of 4HB is realized, and the genetically engineered bacterium is of great significance to promotion of wide application of biopolyester and reduction of negative effects of petroleum-based plastics on the environment.

The present invention provides a primer-probe set, a system, a primer composition and their applications for detecting Photobacterium damselae based on the LFD-RPA technology. The primer-probe set includes primer RAA-yidC-F, primer RAA-yidC-R and probe RAA-yidC-P. The primer-probe set of the present invention has a rapid reaction and a wide temperature range, enabling rapid detection of the target gene. Moreover, the primer-probe set has good sensitivity and specificity for clinical simulated samples, can effectively detect Photobacterium damselae, and provides a rapid detection method available for in vitro diagnosis of Photobacterium damselae. The primer-probe set of the present invention has strong specificity; high detection sensitivity; high accuracy and reliability; simple and rapid operation, suitable for on-site detection, and has a wide range of application scenarios.

The invention relates to a detection method of highly pathogenic photobacterium damsonii subspecies, and belongs to the technical field of detection. The Latin name of the high-pathogenicity photobacterium damsela subspecies is Photobacterium damsela subspecies, and the high-pathogenicity photobacterium damsela subspecies contains a characteristic virulence gene, namely, a hemolysin gene sequence; the method comprises the following steps: detecting a hemolysin gene sequence of the highly pathogenic photobacterium damselysin subspecies by using an enzymatic isothermal amplification (ERA) technology; the primer sequence of the ERA is Pdd-F1; r < d-R < 1 >; according to the direction from the 5'tail end to the 3 'tail end, the nucleotide sequence of the Pdd-F1 is a sequence number (ID): 1; the nucleotide sequence of the Pdd-R1 is shown as a sequence number (ID): 2. The detection method provided by the invention has the characteristics of high specificity, high sensitivity, rapidness and convenience.

The invention provides an indicator gene for the pathogenicity of pomfret infected photobacterium damsonae. The indicator gene comprises a complete nucleic acid sequence and an amino acid sequence. The pomfret proinflammatory factor IL-8 obtained by the invention can be used as an important gene for indicating photobacterium dama infection, and can be applied to preliminary detection of aquaculture bacterial diseases. The pomfret-derived proinflammatory factor IL-8 gene for indicating photobacterium damsonii can be used as an aquatic disease detection preparation for disease prevention and control through a corresponding detection technology.

The invention belongs to the technical field of marine organisms, and particularly relates to application of a gene cluster in detection of photobacterium damsela and a detection primer probe set. The primer probe group is a complete set of primer combination corresponding to three gene loci, and consists of upstream and downstream primers and probe primers; according to the invention, corresponding RPA (recombinase polymerase amplification) detection primers are designed according to three specific gene loci obtained by screening aiming at the existing known photobacterium damsela, and a test strip RPA detection system and method of the photobacterium damsela are established; the technology has an important value for on-site rapid screening and detection of the photobacterium damsonii involved in aquaculture, import and export trade and bathing beaches, and has a wide market application prospect.

The invention belongs to the technical field of microorganisms, and particularly relates to bacillus isothermophilus and application thereof in inhibition of edwardsiella ictaluri and photobacterium damsonii. The invention relates to a strain of bacillus straticola, which is a strain K of bacillus straticola, the strain is preserved in China General Microbiological Culture Collection Center (CGMCC) on February 19, 2025, and the preservation number of the strain is CGMCC No.33567. The invention further discloses a preparation method of the bacillus straticola. The strain can be used as a feed additive for preventing and treating edwardsiella ictaluri and photobacterium damsonii in aquaculture, or can be directly prepared into a feed, and the antibacterial effect is remarkable when the bacterial feed is fed in the aquaculture, or the strain can be added into a water body to be used as a water body disinfectant.

This invention discloses a method for detecting *Proteobacterium melanogaster* subspecies *Melanobacterium merantii*, comprising extracting DNA from the sample to be tested, using the extracted DNA as a template, performing a LAMP amplification reaction using primers, and interpreting the amplification results. The fluorescent LAMP detection kit provided by this invention for *Proteobacterium melanogaster* subspecies *Melanobacterium merantii* exhibits high sensitivity and specificity, facilitating rapid diagnosis of diseases caused by *Proteobacterium melanogaster* subspecies *Melanobacterium merantii* in aquatic animals, and is of great significance for the prevention and treatment of related diseases.

The invention relates to the technical field of biology, and discloses a multiplex PCR (Polymerase Chain Reaction) detection primer combination, a kit and a method for simultaneously detecting multiple marine pathogenic bacteria. Specific primer pairs are respectively designed for marine pathogenic bacteria such as vibrio alginolyticus, vibrio vulnificus, photobacterium dama subspecies, vibrio harveyi and vibrio candersonii, a freeze-drying technology is combined, and the amplification efficiency and specificity are improved through primer design and optimization of an amplification system and detection program parameters. In addition, the method has relatively high accuracy, and shows an efficiency advantage, a relatively short detection period and a convenient result judgment characteristic when being used for detecting various marine pathogenic bacteria. Aiming at the field of detection of diseases caused by various marine pathogenic bacteria, the invention provides a reliable detection way, which is of great significance in practical application scenes such as aquaculture disease monitoring, prevention and control, marine organism health assessment and the like, and is helpful for promoting development and perfection of related disease detection technologies.

The invention belongs to the technical field of environmental microorganisms, and particularly relates to a photobacterium strain and application thereof in straw degradation. The invention discloses a photobacterium strain Photobacterium sedimentimangrovi GSS17 which is preserved in Guangdong Microbial Culture Collection Center on January 11, 2023, and the preservation number of the photobacterium strain Photobacterium sedimentimangrovi GSS17 is GDMCC (China General Microbiological Culture Collection Center) No. 62977. Compared with the prior art, the strain can secrete various active enzymes related to carbohydrate degradation and can accelerate rapid conversion of cellulose and hemicellulose substances so as to promote straw degradation.

The invention belongs to the technical field of microbiological detection, and particularly relates to an isothermal amplification detection primer of photobacterium damsonii subspecies and application of the isothermal amplification detection primer. The primer for loop-mediated isothermal amplification detection of the strain is designed aiming at the ureC gene of the photobacterium damsela subspecies, a quantitative analysis result can be provided by detection, and the primer has the advantages of rapidness, specificity and on-site detection.

The invention provides a method for detecting the mermaid luminescent bacillus mermaid subspecies, comprising extracting DNA from a single colony of the mermaid luminescent bacillus mermaid subspecies or extracting DNA from a fish tissue to be detected, and designing specific primers based on the subspecies level, taking the DNA of the sample to be detected as a template, performing PCR amplification detection through an upstream primer P1 and a downstream primer P2 pair, and performing electrophoresis analysis. The invention can accurately distinguish the mermaid luminescent bacillus mermaid subspecies and the piscivorous subspecies in clinical practice, and has high application value in aquaculture.