3 results about "Photobacterium phosphoreum" patented technology

The invention belongs to the technical field of aquatic product pathogen gene editing, and particularly relates to a target gene knockout method for photobacterium damsela subsp. Piscicida (PDP), which is characterized in that the target gene knockout method for the photobacterium damsela subsp. Piscicida (PDP) is disclosed. The method comprises the following steps: obtaining a DNA fusion fragment with deletion of a target region fragment of a target gene (MshL) by adopting overlapping PCR (Polymerase Chain Reaction), constructing a recombinant vector by using the obtained DNA fusion fragment, and obtaining the target gene knocked-out photobacterium damnieri subspecies fish by taking escherichia coli S17-1 lambda pir as a donor bacterium of a recombinant plasmid and PDP as a recipient bacterium. Specific knockout of the PDP specific gene is achieved for the first time, the knockout efficiency is high (the single-exchange positive rate is 29.2%, and the double-exchange positive rate is 30.8%), the cost is low, and the method is suitable for construction of a PDP specific gene deleted strain and functional research of the specific gene.

This invention discloses a method for detecting *Proteobacterium melanogaster* subspecies *Melanobacterium merantii*, comprising extracting DNA from the sample to be tested, using the extracted DNA as a template, performing a LAMP amplification reaction using primers, and interpreting the amplification results. The fluorescent LAMP detection kit provided by this invention for *Proteobacterium melanogaster* subspecies *Melanobacterium merantii* exhibits high sensitivity and specificity, facilitating rapid diagnosis of diseases caused by *Proteobacterium melanogaster* subspecies *Melanobacterium merantii* in aquatic animals, and is of great significance for the prevention and treatment of related diseases.

The invention belongs to the technical field of environmental microorganisms, and particularly relates to a photobacterium strain and application thereof in straw degradation. The invention discloses a photobacterium strain Photobacterium sedimentimangrovi GSS17 which is preserved in Guangdong Microbial Culture Collection Center on January 11, 2023, and the preservation number of the photobacterium strain Photobacterium sedimentimangrovi GSS17 is GDMCC (China General Microbiological Culture Collection Center) No. 62977. Compared with the prior art, the strain can secrete various active enzymes related to carbohydrate degradation and can accelerate rapid conversion of cellulose and hemicellulose substances so as to promote straw degradation.