Pomfret photobacterium damsela strain and inactivated vaccine

A technology of photobacteria and inactivated vaccines, applied in the direction of bacteria, antibacterial drugs, bacterial antigen components, etc., can solve the problems of destroying ecological balance, drug residues, anorexia, etc., to curb occurrence and epidemic, simple preparation process, reduce stress stimulating effect

Active Publication Date: 2019-06-14
NINGBO UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the treatment of silver pomfret bacterial disease mainly depends on antibiotics. Although it has a certain effect, it cannot fundamentally control the disease, and the fish body is prone to drug resistance, resulting in drug residues in the environment, destroying ecological balance, and threatening the environment. human health and safety
In addition, fish schools are prone to anorexia symptoms during illness, so the method of feeding bait is not ideal

Method used

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  • Pomfret photobacterium damsela strain and inactivated vaccine
  • Pomfret photobacterium damsela strain and inactivated vaccine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 2

[0016] Example 2: Screening of strains

[0017] 1. Sample collection and processing

[0018] 1) The diseased silver pomfret was taken from Gangwan Aquatic Breeding Company in Xiangshan County, Ningbo City, Zhejiang Province. The abdomen of the silver pomfret was cut with sterilized dissection tools, the intestine was taken out, and the fat and blood on the outer wall of the intestine were washed with PBS buffer. clean. Slowly squeeze out the contents of the gastrointestinal tract, and then cut the gastrointestinal tract longitudinally with sterile ophthalmic scissors, and wash several times with PBS buffer to remove the residual intestinal contents. Then use a rubber spatula to slowly scrape off the mucosa of the inner wall of the intestine. Mix the separated endolysate and mucous membrane together, put them in a 10 mL centrifuge tube containing PBS buffer, and mix well.

[0019] 2) Separate contents and mucous membranes for further separation: Use ophthalmological scissors to cut...

Embodiment 3

[0041] Example 3: Detection of the effect of the inactivated vaccine against Photobacterium mermaid CGMCC NO.16907

[0042] Randomly select 60-day-old healthy silver pomfret, soak it in 8% NaCl solution for 5 minutes, and then immediately transfer to the inactivated vaccine prepared in Example 1, and then dilute the silver pomfret with water at a volume ratio of 1:5. The vaccine was obtained by obtaining the Photobacterium sylvestris mermaid vaccine solution, and then the scorpion pomfret was aerated and soaked in the spheroidea mermaid vaccine solution for 1 h. A week later, the vaccine was boosted according to the aforementioned method. After that, the diet was fed normally, and healthy silver pomfret without vaccine soaking was used as a control. After 28 days, soak the inactivated vaccine and the silver pomfret that has not been immersed to 1.5×10 8 The live bacteria of Photobacterium mermaid with a concentration of CFU / ml were challenged. The water was changed every day to r...

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Abstract

The invention provides pathogenic bacteria separated from pomfret intestinal tract affected with photobacterium damsela, wherein the pathogenic bacteria are photobacterium damselae. The photobacteriumdamsela was deposited on December 7, 2018 in China General Microbiological Culture Collection Center located at No. 1 Beichen West Road, Chaoyang District, Beijing China. A process for preparing an inactivated vaccine against pomfret photobacterium damsela is simple, the yield is stable, and the cost is low, and a large number of inactivated vaccines having a high immune effect can be acquired. The inactivated vaccine prepared by the method has an immune protection rate of 70%. The vaccine is a pure biological preparation, has the advantages of safety and environmental protection and does notcause drug residue and environmental pollution, and the method can fundamentally prevent the occurrence and prevalence of the pomfret photobacterium damsela, and provides technical support for the large-scale preparation of the inactivated vaccine against the pomfret photobacterium damsela.

Description

Technical field [0001] The invention belongs to the field of microbial vaccine preparation, and in particular relates to a kind of Photobacterium silver pomfret and an inactivated vaccine prepared by using the strain. Background technique [0002] Silver pomfret (Pampus argenteus) belongs to the order Perciformes, Stromateidae, and Pampus. It is not only one of the main economic fishes along the coast of my country, but also popular among consumers, but also in India and Kuwait. , Iran and other countries also account for a large proportion of the fishery economy. In the past 20 years, due to overfishing and environmental pollution, wild pomfret resources have been shrinking, and the size of the fished pomfret tends to be smaller. Therefore, it is particularly important to carry out artificial breeding and nursery of silver pomfret. Due to the late start of domestic breeding of pomfret, although the bottleneck of artificial reproduction has been broken, it is still a long way fr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A61K39/02A61P31/04C12R1/01
Inventor 余卓龠王亚军张曼杨阳胡佳宝顾玮玮孙弋博匡思雯余娜曹小欢
Owner NINGBO UNIV
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