Compatibility method for acquiring optimal combined biological effect by testing binary mixture

A technology that combines biological and biological effects, used in biological testing, chemiluminescence/bioluminescence, analysis by chemically reacting materials, etc.

Inactive Publication Date: 2011-10-26
TONGJI UNIV
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

[0006] The purpose of the present invention is to provide a method for determining the compatibility of a binary mixture to obtain the best combined biological effect. This method solves the complex problem of how to obtain the best combined biological effect of the binary mixed system according to the changing law of the biological effect of the mixed system for the first time.

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  • Compatibility method for acquiring optimal combined biological effect by testing binary mixture
  • Compatibility method for acquiring optimal combined biological effect by testing binary mixture
  • Compatibility method for acquiring optimal combined biological effect by testing binary mixture

Examples

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Effect test

Embodiment 1

[0074] (1) Determination steps of single compound biological effect concentration

[0075] Add 0.25ml of Luminescent bacteria (purchased from Nanjing Institute of Soil Science, Chinese Academy of Sciences) solution (T3) into a volumetric flask containing 5ml of medium, and cultivate for 12 hours to make a shake flask bacteria solution; then take 0.2ml shake flask bacteria solution to 20ml 3% sodium chloride, aerated and stirred for 40 minutes to make a working bacteria solution. Take an appropriate amount of malononitrile and p-nitrobenzaldehyde to make a standard series of logarithmic gradients, with a volume of 0.8ml, add 0.2ml of working bacteria solution, shake well, expose to poison for 15 minutes, and measure the decrease in light value of the system before and after exposure degree, the concentration at which the inhibition rate is 50% is calculated by interpolation method, which is the EC of the substance (or system) 50 value. Calculated EC 50 values ​​are respectiv...

Embodiment 2

[0109] (1) Determination steps of single compound biological effect concentration

[0110]Add 0.25ml of Luminescent Bacteria solution (T3) to a volumetric flask containing 5ml of culture medium, and cultivate for 12 hours to make a shake flask solution; then take 0.2ml of shake flask solution into 20ml of 3% sodium chloride and aerate Stir for 40 minutes to make a working bacteria solution. Take an appropriate amount of phthalonitrile (compound A) and p-dimethylaminobenzaldehyde (compound B) to form a standard series of logarithmic gradients, with a volume of 0.8ml, add 0.2ml of working bacteria solution, shake well, and infect for 15 minutes. Measure the reduction degree of the light value of the system before and after exposure, and use the interpolation method to calculate the concentration when the inhibition rate is 50%, which is the EC of the substance (or system) 50 value. Calculate the EC of phthalonitrile (A compound) and p-dimethylaminobenzaldehyde (B compound) 50...

Embodiment 3

[0131] (1) Determination steps of single compound biological effect concentration

[0132] Add 0.25ml of luminescent bacteria solution (T3) to a volumetric flask containing 5ml of medium, and cultivate for 12 hours to make a shake flask solution; then take 0.2ml shake flask solution into 20ml of 3% sodium chloride, Gas stirring for 40min to make working bacteria liquid. Take an appropriate amount of acetonitrile (substance A) and terephthalaldehyde (substance B) to form a standard series of logarithmic gradients, with a volume of 0.8ml, add 0.2ml of working bacteria solution, shake well, expose for 15 minutes, and measure the system before and after exposure. The reduction degree of light value, the concentration at which the inhibition rate is 50% is calculated by interpolation method, which is the EC of the substance (or system) 50 value. Calculated EC 50 The values ​​are respectively 0.189, 6.892×10 -5 mol / L.

[0133] (2) Determination of the joint biological effect of...

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Abstract

The invention belongs to the field of environmental protection, and discloses a compatibility method for acquiring an optimal combined biological effect by testing a binary mixture. The method comprises the following steps: (1) the testing method is based on GB/T 15441-1995, and photobacterium phosphoreum is used as an indicator organism to test the single toxicity EC50-A and EC50-B of A and B compounds; (2) according to the EC50-A and the EC50-B, a mixed solution with an equivalent effect ratio is prepared by a testing method which is based on GB/T15441-1995, the biological effect concentration of the mixed solution is tested to obtain the concentration CA and CB of A and B in a mixed system when inhibition of the mixed system to the biological effect is 50 percent, and a combined biological effect index TU1:1 is calculated in the equivalent effect ratio; and (3) a combined biological effect regulation of the binary mixed system can be predicted according to the testing result TU1:1 of the combined biological effect, which is calculated in the equivalent effect ratio, and the optimal biological effect point can be acquired. The method can be applied in the aspects of environmental science, medication, pesticide and the like.

Description

technical field [0001] The invention belongs to the field of environmental protection, and in particular relates to a compatibility method for determining the optimal joint biological effect of a binary mixture. Background technique [0002] With the rapid development of modern science and technology and the continuous improvement of industrialization, chemicals are ubiquitous in human life, and often exist in a mixture of multiple chemicals, which have had a profound impact on the environment and human health. [0003] In the past 40 years, the quantitative structure-activity relationship (QSAR) method has been used to study the environmental behavior and biological effects of a single compound in considerable detail, and many corresponding results have been obtained. On this basis, many scholars have studied the biological effects of mixed compounds and proposed some prediction methods. [0004] Sprague (Sprague, J.B. "Lethal levels of mixed copper zinc solutions for juve...

Claims

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Application Information

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IPC IPC(8): G01N33/53G01N21/76
Inventor 林志芬田大勇尹大强曾鸣邹小明
Owner TONGJI UNIV
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