Method for detecting and evaluating tetracycline environmental risk by using paramecium biomarkers and IBR

A technology of biomarkers and tetracyclines, applied in biological testing, measuring devices, material inspection products, etc., can solve the problems of long cycle, insufficient quantification, and low degree of standardization

Active Publication Date: 2021-12-10
HARBIN INST OF TECH SHENZHEN GRADUATE SCHOOL
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] (1) In the past, the species of tetracycline biological detection were not sensitive enough or the cost was high, the cycle was long, and the degree of standardization was not high;
[0009] (2) The types of biomarkers used in the past are few, qualitative and quantitative;
[0010] (3) The detection concentration range and the actual concentration in the environment are not highly targeted, and the practicability is low;
[0011] (3) Lack of comprehensive analysis and evaluation of IBR
[0014] However, for the environmental concentration and environmental risk assessment of specific substances, a large amount of research is needed to establish, how to quickly and accurately assess the environmental concentration and environmental risk of tetracycline is the problem to be solved by the present invention

Method used

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  • Method for detecting and evaluating tetracycline environmental risk by using paramecium biomarkers and IBR
  • Method for detecting and evaluating tetracycline environmental risk by using paramecium biomarkers and IBR
  • Method for detecting and evaluating tetracycline environmental risk by using paramecium biomarkers and IBR

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Embodiment 1

[0054] 1. Materials and methods

[0055] 1.1 Indicator organisms

[0056]Paramecia (Parameci μm ca μdat μm), Cilia, Oligomembrane, Hymenostomy, Paramecia, Paramecia genus. Widely distributed in still water bodies rich in organic matter. It is sensitive to the stimulation of environmental pollutants, so it is often used in the evaluation research of environmental pollutants.

[0057] 1.2 Cultivation of Paramecium

[0058] It can be collected in fresh water bodies rich in plankton, pick single cells, wheat grain culture medium, establish monoclonal culture lines in a constant temperature and humidity incubator at 20-25°C, starvation-induced conjugation, large-scale pure culture, and logarithmic growth Phase individuals are used for tetracycline testing.

[0059] 1.3 Setting of detection concentration range of tetracycline

[0060] According to various literature reports and environmental monitoring reports, the experimental concentration of tetracycline in the water and soi...

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Abstract

The invention belongs to the technical field of biological monitoring and evaluation of antibiotic environmental pollution, and particularly relates to a method for detecting and evaluating tetracycline environmental risk by using paramecium biomarkers and IBR, wherein tetracycline environmental concentration and environmental risk are rapidly evaluated by using various biomarkers of sensitive indicator organisms and IBR integrated analysis.

Description

technical field [0001] The invention belongs to the technical field of biological monitoring and evaluation of antibiotic environmental pollution, and in particular relates to a method for detecting and evaluating tetracycline environmental risks by using paramecium biomarkers and IBR. Background technique [0002] The test species, detection concentration range, detection parameters and whether IBR is used for integrated analysis in the tetracycline biological detection method reported in the prior art are as follows: [0003] [0004] [0005] For example, CN201110271631.3 is an evaluation method for the soil environment safety of transgenic insect-resistant rice, which uses the soaking solution of transgenic insect-resistant rice straw to cultivate paramecium, and determines whether the transgenic rice has no effect on the growth of paramecium by counting the cell density of the cultured paramecium. , reproduction, etc.; then use comet electrophoresis technology to ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/50G01N33/15
CPCG01N33/5008G01N33/15
Inventor 陈瑛任南琪尹笑宇
Owner HARBIN INST OF TECH SHENZHEN GRADUATE SCHOOL
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