3 results about "EC50" patented technology

The invention discloses an anti-human OX40 inhibitory antibody or an antigen binding fragment thereof, a preparation method and application, and belongs to the technical field of biological immunity. The EC50 of the human-mouse chimeric antibody Y07-L2 and the EC50 of the combination of the humanized antibody Y07-C2 and the human OX40, which are prepared by the invention, are close to those of a targeted antibody drug KHK-4083 for inhibiting the OX40. A heavy chain variable region of the human-mouse chimeric antibody Y07-L2 is as shown in SEQ ID NO.14, and a light chain variable region of the human-mouse chimeric antibody Y07-L2 is as shown in SEQ ID NO.15; the heavy chain variable region of the humanized antibody Y07-C2 is as shown in SEQ ID NO.20, and the light chain variable region of the humanized antibody Y07-C2 is as shown in SEQ ID NO.21; the CDR region of the humanized antibody Y07-C2 is mutated, and the mutant antibodies Y07-C2MH4 and Y07-C2MH4L3 are obviously superior to other antibodies.

The application discloses an inhibitory antibody or antigen-binding fragment thereof against human OX40, a preparation method and application, and belongs to the technical field of biological immunity. The human-mouse chimeric antibody Y07-L2 and the humanized antibody Y07-C2 prepared by the application have similar EC50 in combination with human OX40, and are similar to the targeted antibody drug KHK-4083 in inhibiting OX40. The heavy chain variable region of the human-mouse chimeric antibody Y07-L2 is shown as SEQ ID NO. 14, and the light chain variable region is shown as SEQ ID NO. 15; the heavy chain variable region of the humanized antibody Y07-C2 is shown as SEQ ID NO. 20, and the light chain variable region of the humanized antibody Y07-C2 is shown as SEQ ID NO. 21; the CDR region of the humanized antibody Y07-C2 is subjected to mutation, and the mutant antibody Y07-C2MH4 and Y07-C2MH4L3 are obviously superior to other antibodies.

The invention relates to the technical field of environment detection, in particular to application of pentachlorophenol as a standard reference substance to detection of interference effect of an environment sample on a retinoic acid receptor beta. The pentachlorophenol toxicity equivalent concentration of the triggering value of the interference effect is 10.77 [mu] g / L. A standard reference compound pentachlorophenol for the in-vitro biological test of the retinoic acid receptor beta interference effect of the water environment sample is screened out, the compound is high in detection rate, has a good linear interval on a dose-effect relation curve of the retinoic acid receptor beta interference effect, is beneficial to calculation of the toxicity equivalent, and has a good application prospect in the field of in-vitro biological test of the retinoic acid receptor beta interference effect. The problem that only the EC20 value or the EC50 value of the water environment sample can be calculated in the previous research is solved, and the method is beneficial to visual display of toxicity results and data use of an environment management department. The application also reveals that the pentachlorophenol toxicity concentration corresponding to the background value of the interference effect of the retinoic acid receptor beta of surface water in China is 6.90 [mu] g / L.