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Kit for measuring acute toxicity of aqueous substances by using large siphonaptera, and manufacturing method and using method thereof

A technology for acute toxicity and large fleas, applied in the field of reagents, can solve the problems of complex experimental measurement procedures, low detection comparability, and inability to flexibly control the measurement time, so as to reduce detection errors, reduce detection errors, and save manpower and economic costs. Effect

Active Publication Date: 2011-09-14
BEIJING DRAINAGE GRP CO LTD
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

This method must ensure that the laboratory always cultures and subcultures Daphnia magna, the experimental measurement procedure is complicated, the measurement time cannot be flexibly controlled, and the detection comparability is not high

Method used

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  • Kit for measuring acute toxicity of aqueous substances by using large siphonaptera, and manufacturing method and using method thereof
  • Kit for measuring acute toxicity of aqueous substances by using large siphonaptera, and manufacturing method and using method thereof
  • Kit for measuring acute toxicity of aqueous substances by using large siphonaptera, and manufacturing method and using method thereof

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Embodiment Construction

[0035] The present invention will be further described below in conjunction with the accompanying drawings and embodiments. Embodiment: The acute toxicity test kit of Daphnia magna to water quality substances is provided with a box shell 1, the upper part of the shell is provided with an upper open box cover 3, and the rear part of the shell is provided with a removable 30-hole 21 test plate 2 and an incubation dish 5. A dropper and a screen 4 are provided between the test plate and the hatching dish, and several diluted jellyfish liquid bottles 8, bait bottles 7, and magna egg bottles 6 are arranged in sequence from left to right in the front of the box.

[0036] The preparation method embodiment of kit of the present invention: make according to the following steps:

[0037] 1) Preparation of pure strain Daphnia magna larvae:

[0038] (1) Pick young fleas: select the young fleas that have been cultivated for more than 3 generations under laboratory conditions and have been ...

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Abstract

The invention discloses a kit for measuring acute toxicity of aqueous substances by using large siphonaptera, and a manufacturing method and a using method thereof. The kit is provided with a shell, a test board, an incubation vessel, a dropper, a screen, a diluted jellyfish liquid bottle, a bait bottle and a large siphonaptera bottle. The manufacturing method of the kit comprises the following steps of: 1, preparing larvae of the pure strain large siphonaptera; 2, feeding the larvae; 3, separating adult siphonaptera; 4, preparing resting eggs; 5, purifying and storing the resting eggs; 6, preparing baits; 7, preparing diluted jellyfish liquid; and 8, manufacturing accessories and placing the products into boxes. The using method of the kit comprises the following steps of: 1, preparing standard diluted water; 2, incubating eggs of the large siphonaptera; 3, according to a result of a preliminary test, determining the concentration range of an official test of measuring the acute toxicity of the aqueous substances by using the large siphonaptera; 4, performing blank control; 5, starting to perform the test of measuring the acute toxicity of the aqueous substances by using the large siphonaptera; and 6, calculating the result, namely estimating semi-effect concentration EC50. The invention is suitable for measuring the acute toxicity of the aqueous substances by using the largesiphonaptera, low in cost, convenient in use and accurate in measurement.

Description

technical field [0001] The invention relates to a reagent, in particular to a kit for measuring the acute toxicity of water substances by using Daphnia magna and its production method and application method. Background technique [0002] At present, the reagents used in the determination of the acute toxicity of water substances by Daphnia magna require that the source of the Daphnia magna (Daphnia magna), that is, the Daphnia magna of the Crustacea Cladocera, is to maintain good culture conditions to make the Daphnia magna Reproduction was controlled under the parthenogenetic state, and young fleas cultured for more than 3 generations under laboratory conditions and born 6 to 24 hours were selected as test fleas. This method must ensure that the laboratory always cultures and subcultures Daphnia magna. The experimental measurement procedure is complicated, the measurement time cannot be flexibly controlled, and the detection comparability is not high. Therefore, there is a...

Claims

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Application Information

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IPC IPC(8): G01N33/18
Inventor 李魁晓宫飞蓬周军甘一萍
Owner BEIJING DRAINAGE GRP CO LTD
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