Method for predicating embryotoxicity of non-steroidal anti-inflammatory drug type novel pollutants on early-phase life stage of zebra fish

Abstract

The invention discloses a method for predicating the embryotoxicity of non-steroidal anti-inflammatory drug type novel pollutants on an early-phase life stage of zebra fish. The method comprises the following steps: exposing zebra fish embryos in the non-steroidal anti-inflammatory drug type novel pollutants which have equal logarithm space concentrations; recording the death rate and the aberration rate of the zebra fish embryos 7 days after exposing; and calculating by using SPSS software to obtain corresponding LC50 and teratogenetic EC50, which are used for evaluating the toxicity of the non-steroidal anti-inflammatory drug type novel pollutants. With the adoption of the method, the toxicity feature and the toxicity level of the non-steroidal anti-inflammatory drug type novel pollutants are subjected to an analytical test and quantitative description; and meanwhile, the method also can be used as an index for monitoring and evaluating the wastewater biological toxicity of non-steroidal anti-inflammatory drugs, and reference is provided for risk predication and evaluation of the potential biological toxicity of the pollutants in a water body.

Description

Technical field

[0001] The invention belongs to the technical field of environmental toxicology, and in particular relates to a method for predicting the embryo toxicity of novel non-steroidal anti-inflammatory drugs to zebrafish in the early life stage. Background technique

[0002] The widely used non-steroidal anti-inflammatory drugs (NSAIDs) not only relieve human suffering, but also bring many environmental pollution and hazards. Exposure to lower concentrations will cause adverse effects on aquatic organisms, and they may also be synergistic and additive. Such interactions lead to increased joint toxicity when a large amount of coexistence occurs, and the potential risks cannot be ignored. However, the current toxicological studies of non-steroidal anti-inflammatory drugs are still mainly focused on the acute toxicity of short-term high-dose exposures simulated in the laboratory, and there are few studies on the toxicity that may be caused by exposure to their actual enviro...

Examples

Embodiment 1

[0020] Example 1: Evaluation of three typical non-steroidal anti-inflammatory drugs and novel pollutants (diclofenac, acetaminophen and ibuprofen) on the embryo toxic effects of zebrafish in early life stages

Technical field

[0001] The invention belongs to the technical field of environmental toxicology, and in particular relates to a method for predicting the embryo toxicity of novel non-steroidal anti-inflammatory drugs to zebrafish in the early life stage. Background technique

[0002] The widely used non-steroidal anti-inflammatory drugs (NSAIDs) not only relieve human suffering, but also bring many environmental pollution and hazards. Exposure to lower concentrations will cause adverse effects on aquatic organisms, and they may also be synergistic and additive. Such interactions lead to increased joint toxicity when a large amount of coexistence occurs, and the potential risks cannot be ignored. However, the current toxicological studies of non-steroidal anti-inflammatory drugs are still mainly focused on the acute toxicity of short-term high-dose exposures simulated in the laboratory, and there are few studies on the toxicity that may be caused by exposure to their actual enviro...

Examples

Embodiment 1

[0020] Example 1: Evaluation of three typical non-steroidal anti-inflammatory drugs and novel pollutants (diclofenac, acetaminophen and ibuprofen) on the embryo toxic effects of zebrafish in early life stages

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