The invention discloses extract having bactericidal activity from Fraxinus hubeiensis and a bactericidal composition comprising the same; the extract is prepared by: crushing root, stem, leaf, flower and / or seed of Fraxinus hubeiensis, extracting with a leaching agent at room temperature to obtain extracted material, concentrating the extracted material under reduced pressure until zero solvent is present to obtain crude extract, diluting the crude extract with distilled water, performing multiple extraction with an extracting agent to obtain liquid extract, concentrating the liquid extract, and drying to obtain the bactericidal activity extract from Fraxinus hubeiensis, wherein the leaching agent is selected from water, alcohols, esters, ketones, ethers, chloralkanes, arenes, or alkanes; the extracting agent is selected from esters, ketones, ethers, chloralkanes, arenes, or alkanes. The extract, as a compound, according to the invention has wide-spectral insecticidal and bactericidal activity, and has significant bacterial and inhibitory effects upon wheat scab, rice sheath blight, cotton boll rot, walnut root rot, potato early blight, apple altermaria leaf spot, ginseng black spot, cotton damping-off and the like.