44results about How to "Broad-spectrum bactericidal activity" patented technology

The invention relates to a disinfectant prepared from plant extracts. The disinfectant prepared from the plant extracts comprises the following raw materials in percentage by weight: 0.05 to 0.5 percent of chlorogenic acid, 0.1 to 1 percent of gallic acid, 0 to 1 percent of sophocarpidine, 0 to 0.8 percent of clove oil, 0 to 1 percent of peppermint oil, 0 to 1 percent of moxa volatile oil or spearmint oil, 0.1 to 2 percent of tween 80, 5 to 50 percent of ethanol, 0.1 to 1 percent of sodium citrate, 0 to 0.8 percent of lauryl sodium sulfate, 0 to 0.5 percent of fragrance regulator and the balance of pure water. The invention also provides a preparation method for the disinfectant. The disinfectant prepared from the plant extracts and the preparation method for the disinfectant have the beneficial effects that: the disinfectant belongs to a pure Chinese medicine compound disinfectant preparation, is safe for human and livestock to use, does not have corrosivity and toxic or side effect, is insusceptible to medicine resistance after a long-time use, has good disinfection effect, avoids environmental pollution, has wide application, can be used for disinfecting air and can also be used for disinfecting the surfaces of skin, clothes, tableware, furniture and the like, and meets universal requirements of people.

The invention discloses an application of alpha-mangostin in controlling plant diseases. Tests prove that alpha-mangostin has agricultural fungicidal activity. The compound has very strong fungistatic action on mango anthracnose pathogens, diplodina sp., botrytis cinerea, and exserohilum turcicum when the testing concentration is 100 mg/L, and has an EC50 value of 18 mg/L-35 mg/L; the compound has certain spore germination inhibition effect on exserohilum turcicum and botrytis cinerea, and the EC50 values are 66.20 mg/L and 50.43 mg/L respectively; meanwhile, in-vivo test results show that the compound has very good protection and treatment effects on rubber tree powdery mildew when the testing concentration is 500 mg/L, and the protection and treatment effects on rubber tree powdery mildew are 75.61% and 70.12% respectively, which are higher than those of a control drug of prochloraz. The above results indicate that alpha-mangostin has broad-spectrum fungistatic action on plant pathogenic fungi, which lays the foundation for the development of novel pollution-free plant-derived pesticides by further using mangosteen shells.

The invention belongs to the technical field of pathogenic bacterium control, and particularly relates to an N'-substituted benzal-1,2,3,4-tetrahydroquinoline base-1-(sulfo)hydrazide compound and a preparation method and application thereof. By substituting 1,2,3,4-tetrahydroquinoline fragments excellent in biological activity into a substituted benzaldehyde thiosemicarbazone compound, the N'-substituted benzal-1,2,3,4-tetrahydroquinoline base-1-(sulfo)hydrazide compound novel in structure is obtained. The structure of the compound is seen in the formula I (please see the formula in the specification). The preparation method is easy to operate and free of harmful by-products, and raw materials are easy to obtain. The compound has broad-spectrum bactericidal activity, particularly has an obvious restraining effect on Valsa mali, rhizoctonia solani and gaeumannomyces gramini, and can be used as a bactericide for preventing and curing plant diseases.

The invention discloses a fatty acid complex. The fatty acid complex comprises the following components in parts by weight: 2.2-3 parts of mixed fatty acid, 16-40 parts of a surfactant and 40-60 partsof auxiliary acid, wherein the mixed fatty acid is a mixture of octanoic acid, nonoic acid and capric acid; the auxiliary acid is lauric acid or a mixture of the lauric acid and citric acid. The fatty acid complex has broad-spectrum bactericidal and antiviral activity, can kill polioviruses, foot-and-mouth disease viruses, porcine reproductive and respiratory syndrome viruses and avian influenzaviruses, has the staphylococcus aureus and escherichia coli killing rate as high as 99.99999%; the fatty acid complex has excellent storage stability under a diluted condition, is applied to a food disinfectant, has an excellent food preserving function, is free of cleaning during disinfection, free of metal corrosion, practically non-toxic and non-irritant to skin, and is unlikely to volatilize;the fatty acid complex has acidic pH and a light coconut scent, disinfecting cleaning sewage does not pollute the environment, and the fatty acid complex can be reused in some disinfecting scenes.

The invention specifically discloses ferment leaf fertilizer for green vegetables. The ferment leaf fertilizer can improve the absorption rate of a leaf surface for nutrients, promotes photosynthesis, enables the leaf to grow vigorously, and has an antibacterial insect repelling efficacy. The ferment leaf fertilizer is prepared by mixing a solution A and a solution B according to a weight ratio of 1: (1.0 to 1.3), the pH value of the mixed solution is adjusted to 6.0 to 6.5 by adding acid, wherein the solution A is prepared by fermenting the following components for 3 to 5 months at a temperature of 25 to 35 DEG C and removing the ferment residues: brown sugar, sodium chloride, silkworm excrement, tea seed cake, fresh fruits and vegetable residues, water, activated EM flora and bacillus aceticus; and the solution B is prepared by adding water to the following components to 100 parts: biochemical fulvic acid type B, sugar alcohol, urea, boric acid, ammonium sulfate, zinc sulfate, magnesium sulfate, manganese sulfate, ferrous sulfate, ammonium molybdate, potassium dihydrogen phosphate and a penetrating agent.

The invention discloses a Chinese violet coumarin with anti-inflammatory and antiseptic activity and a preparation method and application thereof. The preparation method of the Chinese violet coumarin with the anti-inflammatory and antiseptic activity comprises the following steps of (1) drying Chinese violet residues at 45 DEG C and crushing the dried Chinese violet residues into Chinese violet powder; (2) irradiating the Chinese violet powder under ultraviolet rays for 30 min for sterilization; (3) adding water, 3 times as much as the weight of the sterilized Chinese violet powder, into the sterilized Chinese violet powder, and then adding in complex enzymes, uniformly mixing and placing the mixture in a 55 DEG C thermostatic water bath for 1.5 h; (4) adding organic extracting agent into the Chinese violet powder and then performing reflux extraction twice for 35 min each time, and performing decompressed vacuum filtration, concentration and vacuum drying to obtain the Chinese violet coumarin. The prepared Chinese violet coumarin with the anti-inflammatory and antiseptic activity has broad-spectrum bacteriostatic, bactericidal and anti-inflammatory activity.

The invention discloses an azophenylene-1-bishydrazide carboxylate compound. The azophenylene-1-bishydrazide carboxylate compound is characterized by being represented by a general formula (I), wherein the structural formula is as shown in the specification, and R is substituted phenyl containing one or more saturated or unsaturated hydrocarbyl groups, alkoxy, halogens, fluorine-containing methyl, nitro, cyano, ester group, keto and formyl group, substituted pyridyl, substituted furyl, substituted pyrazolyl, substituted thiazolyl and substituted thienyl. The compound disclosed by the invention simultaneously has broad spectrum insecticidal and bacterial activities, and can be used for controlling diseases caused by multiple pathogenic bacteria such as oomycete, basidiomycetes, ascomycetes, imperfect fungi and the like on various crops.

The invention discloses a pineapple preservative and a preparation method thereof and belongs to the technical field of fruit and vegetable fresh keeping. The pineapple preservative is prepared by mainly mixing the raw materials of a plant extract and auxiliary materials according to weight proportion, wherein the plant extract is a mixed water extract including longan leaves, lemon leaves, liquorice, honeysuckle and garlic; the auxiliary materials comprise enestroburin, kresoxim-methyl, potassium sorbate, propolis, starch and sodium carbonate. The pineapple preservative is mainly prepared by extracting natural plant elements and synthesizing according to proportioning, reduces or avoids the addition of chemicals and is safe and reliable; the fresh-keeping time is long, the use is convenient, and meanwhile, certain effects such as scar repair of fruits can be achieved; the raw materials can be conveniently obtained and are low in cost, the preparation technology is simple, an obvious fresh-keeping effect can be taken, and the pineapple preservative has wide popularization and market prospects.

The invention provides phenazine-1-carboxylic acid bishydrazide compounds and an application thereof, and belongs to the technical field of preparation of pesticide compounds; the general formula of the compounds has the following structure defined in the specification, wherein R is saturated C2-C6 straight-chain or branched-chain alkyl, unsaturated C2-C6 alkyl, halogen substituted C2-C6 hydrocarbonyl, C3-C8 cycloalkyl, and substituted phenyl containing one or more saturated or unsaturated hydrocarbonyl, alkoxy, halogen, fluorine-containing methyl, nitryl, cyano group, ester group, ketone group and formyl group, substituted pyridyl, substituted furyl, substituted pyrimidyl, substituted pyrazolyl, substituted thiazolyl, substituted thienyl, substituted imidazolyl or substituted triazolyl. The phenazine-1-dihydrazine carboxylic acid compounds have novel structure, can be used for prevention and treatment of diseases on crops and weeds and have market application prospects.

Disclosed are a compound 2-hydroxy-3-fluoro-5-nitro-1-phenyl butanone and a preparation method and an agricultural biological activity thereof. The preparation method comprises the steps: adding nitric acid, acetic anhydride and methanol in a reactor, after stirring for a period of time, cooling the reaction liquid to 0-20 DEG C, adding 2-hydroxy-3-fluoro-1-phenyl butanone, carrying out a reaction for a period of time, adding water into the reaction mixture, then adding ethyl acetate, extracting for multiple times, and thus obtaining the target compound from an organic phase. The compound has relatively high inhibitory effects on apple rot, Chinese cabbage grey mould, citrus anthrax, cotton fusarium wilt, wheat take-all and other plant pathogenic bacteria.

The invention belongs to the technical field of pathogenic bacteria control, and particularly relates to an oxime ether (ester) compound as well as a preparation method and application thereof. According to the invention, a 1, 2, 3, 4-tetrahydroquinoline or indole fragment with excellent biological activity is introduced into an oxime ether (ester) compound to obtain a compound with a novel structure. The structure of the compound is shown as a formula I. The compound has broad-spectrum bactericidal activity, particularly has an obvious inhibition effect on rhizoctonia solani, botrytis cinerea and valsa mali, and can be used as a bactericide for preventing and treating plant diseases.

The invention provides an endolytic enzyme and perforin composition for resisting colibacteriophage expression as well as a preparation method and application thereof, and belongs to the technical field of bioengineering. An amino acid sequence of endolytic enzyme is as shown in SEQ ID NO:1, and an amino acid sequence of perforin is as shown in SEQ ID NO:2. The endolytic enzyme Lys 9 and the perforin Hol 9 expressed by bacteriophage have broad-spectrum bactericidal activity, have a relatively good antibacterial effect on various gram-positive bacteria and gram-negative bacteria, and have an obvious killing effect on escherichia coli; the endolytic enzyme Lys 9 has an N-terminal enzyme activity structural domain and can dissociate beta-1,4-glucosidic bonds; the perforin Hol 9 has a conservative structural domain, can destroy cells and transmits information to control the lysis time of the cells; and the composition can be used for preparing medicines for preventing, inhibiting or treating cell infection, and has popularization and application value in the fields of medical treatment and food safety.

The invention relates to a plectasin mature polypeptide dimer fusion protein, genes for coding the protein and an expression vector containing a DNA sequence for coding the protein. The invention also relates to a method for expressing the plectasin mature polypeptide dimer fusion protein in recombinant Pichia pastoris. The method comprises the following steps: using the expression vector containing the DNA sequence for coding the plectasin mature polypeptide dimer fusion protein to convert Pichia pastoris and obtain recombinant Pichia pastoris, and performing fermentation culture to secrete the plectasin mature polypeptide dimer fusion protein. The obtained plectasin mature polypeptide dimer fusion protein has bioactivity and can be widely used in the fields such as medical treatments, foods, feeds and scientific researches.

The invention discloses an antibiotic-free milk replacer, which is prepared from the following raw materials in parts by weight: 21.8 parts of common corn, 15 parts of extruded corn, 20 parts of broken rice, 5 parts of dehulled soybean meal, 2 parts of dietary fiber, 5 parts of low-protein whey powder, 6 parts of extruded full-fat soybeans, 4 parts of fish meal, 5 parts of potato protein powder, 2 parts of emulsified oil, 0.55 part of organic calcium, 1 part of monocalcium phosphate, 2 parts of glucose, 2.5 parts of white sugar, 0.15 part of choline chloride, 2 parts of dried porcine solubles, 3 parts of plasma and 3 parts of a nucleus material. The invention provides a milk replacer capable of controlling suckling pig diarrhea without an antibiotic. The milk replacer is capable of preventing piglet diarrhea by selecting kinds of easy-to-digest raw materials with low stress and low antigen and a plurality of intestinal health technologies, and the effect is relatively obvious.

The invention provides a preservative for wood transportation. The preservative for wood transportation comprises the following raw materials in parts by weight: 35 to 52 parts of ketamine, 26 to 34 parts of ammoniacal copper quats, 16-28 parts of zinc dimethyldithiocarbamate or kresoxim-methyl, 56-63 parts of ammonium carbamate, 43 to 51 parts of 2,4,5,6-tetrachloro-3-benzenedicarbonitrile, 2-5 parts of boron carbide and 2 to 5 parts of iron boride. The preservative activity of the preservative for wood transportation is ensured through the adoption of the ammoniacal copper quats; the capability of resisting damage by worms or vermin is ensured through the adoption of the ammonium carbamate; mildew resistance, and stability of the preservative under the condition of illumination and rainwater is ensured through the adoption of the 2,4,5,6-tetrachloro-3-benzenedicarbonitrile; the oxidation resistance and the stability of the preservative for wood transportation can be improved through the adoption of the boron carbide and the iron boride.

The invention discloses an aryl-substituted thiosemicarbazone compound and a preparation method and application thereof. The structural formula of the compound is as shown in Formula I. The preparation method comprises the steps of: mixing a compound of the formula II and a compound of the formula III in an organic solvent to carry out a condensation reaction to obtain the compound of the formulaI. A salt of the aryl-substituted thiosemicarbazone compound is obtained by reacting the aryl-substituted thiosemicarbazone compound with an acid. The compound and the salt thereof are used in the preparation of a medicament for controlling phytopathogen which is harmful to agricultural production or controlling the phytopathogen which is harmful to agricultural production. The compound has novelstructure and simple preparation method, and has a broad spectrum of bactericidal activity.

The invention provides an environment-friendly coating with heat insulation and corrosion resistance, and relates to the technical field of novel coatings. The environment-friendly coating with the heat insulation and corrosion prevention functions is prepared from the following raw materials in parts by weight: 40 to 48 parts of acrylic resin, 0.6 to 0.8 part of acryloyloxyethyl dimethyl ethyl ammonium bromide, 0.5 to 0.6 part of isothiazolinone, 0.5 to 0.8 part of ultraviolet light absorber, 3 to 5 parts of modified far infrared ceramic powder, 0.4 to 0.8 part of coalescing agent, 0.1 to 0.3 part of dispersing agent, 0.1 to 0.2 part of defoaming agent, 2 to 3 parts of kaolin, 1 to 3 parts of talcum powder and 6 to 9 parts of deionized water. By adding acryloyloxyethyl dimethyl ethyl ammonium bromide and isothiazolinone into the coating, the coating can have the antibacterial characteristic, is not easily influenced by microorganisms and is better in corrosion resistance, by adding the modified far infrared ceramic powder into the coating, the multifunctional heat insulation effect of isolation, reflection and radiation can be achieved, the heat insulation effect is better, and vigorous popularization is worthy.

The invention discloses a volatile extract with bactericidal activity in fraxinus hupehensis and a bactericidal composition thereof. The extract is prepared by the following method: after carrying out crushing treatment on roots, stems, leaves, flowers and/or seeds of the fraxinus hupehensis at -5 DEG C to 0 DEG C; adding crushed materials into a simultaneous distillation-extractor (SDE) and carrying out distillation and extraction at a temperature of 50 DEG C to 150 DEG C for 1h-72h; cooling to room temperature and standing and layering; taking an organic phase and dehydrating the organic phase with a drying agent; filtering to remove the drying agent, and removing an extracting solvent in vacuum at a temperature of -100 DEG C to 30 DEG C to obtain the volatile extract with the bactericidal activity in the fraxinus hupehensis, wherein an extracting agent used in a distillation and extraction process is selected from ester, ketone, ether, chloralkane, aromatic hydrocarbon or alkane. The volatile extract disclosed by the invention has wide-spectrum insecticidal and bactericidal activity and can be used for preventing and treating diseases and insect pests including rice sheath blight, wheat head blight, potato early blight, walnut root rot disease, rape sclerotinia rot and the like.

The invention belongs to the technical field of farm chemicals, and specifically relates to application of 3,6-disubstituted imidazo[1, 2-b] pyridazine derivatives in preparation of fungicides. The 3,6-disubstituted imidazo[1, 2-b] pyridazine derivatives are prepared and applied in farm chemical preparation. The inhibition activity of the derivatives on nine common plant pathogenic fungi is testedby adopting a hypha growth rate inhibition method; some of the derivatives show excellent fungistat activity, and are expected to be used for preventing and treating plant diseases caused by pathogenic fungi such as wheat scab, rice blast, cotton blight, tobacco brown spot, cabbage black spot, pumpkin blight, apple ring spot, Curvularia lunata(Walk) Boed and potato dry rot.