Plectasin mature polypeptide dimer fusion protein and preparation method thereof

A peptide dimer, fusion protein technology, applied in the field of genetic engineering
CN101851280AInactive Publication Date: 2010-10-06THE INST OF BIOTECHNOLOGY OF THE CHINESE ACAD OF AGRI SCI +1
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Patent Information

Authority / Receiving Office
CN Β· China
Patent Type
Applications(China)
Current Assignee / Owner
THE INST OF BIOTECHNOLOGY OF THE CHINESE ACAD OF AGRI SCI
Publication Date
2010-10-06
Estimated Expiration
Not applicable Β· inactive patent

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Abstract

The invention relates to a plectasin mature polypeptide dimer fusion protein, genes for coding the protein and an expression vector containing a DNA sequence for coding the protein. The invention also relates to a method for expressing the plectasin mature polypeptide dimer fusion protein in recombinant Pichia pastoris. The method comprises the following steps: using the expression vector containing the DNA sequence for coding the plectasin mature polypeptide dimer fusion protein to convert Pichia pastoris and obtain recombinant Pichia pastoris, and performing fermentation culture to secrete the plectasin mature polypeptide dimer fusion protein. The obtained plectasin mature polypeptide dimer fusion protein has bioactivity and can be widely used in the fields such as medical treatments, foods, feeds and scientific researches.
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Description

technical field

[0001] The invention belongs to the field of genetic engineering, and in particular relates to a fusion protein of a pseudosigaldin mature polypeptide dimer and a preparation method thereof. Background technique

[0002] Antimicrobial peptides (AMPs) refer to a class of small molecule polypeptides composed of 12-100 amino acids, with a net positive charge, amphipathic and antibacterial activity. The research on antimicrobial peptides has a history of more than 50 years. So far, about 880 antimicrobial peptides from different host sources have been identified (Brogden K.A., 2005). Antimicrobial peptides have attracted the attention of many researchers due to their broad-spectrum antibacterial activity (including against Gram-positive bacteria, Gram-negative bacteria, fungi, and even viruses). Especially in recent years, as the abuse of antibiotics has led to the continuous emergence of antibiotic-resistant pathogenic bacteria, it is urgent to develop a new ge...

Claims

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