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Litsea cubeba essential oil and preparation method and application thereof

A technology of Litsea Cubeba and essential oils, applied in the direction of essential oils/spices, fat production, etc., can solve problems such as large randomness, achieve good functional characteristics, good market application prospects, and easy-to-obtain effects

Inactive Publication Date: 2018-10-09
RES INST OF SUBTROPICAL FORESTRY CHINESE ACAD OF FORESTRY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, the Litsea Cubeba industry has no widely used fine varieties, and the extraction of Litsea Cubeba Essential Oil in the industry is not limited to Litsea Cubeba Provenance and strains, and the randomness is often relatively large

Method used

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  • Litsea cubeba essential oil and preparation method and application thereof
  • Litsea cubeba essential oil and preparation method and application thereof
  • Litsea cubeba essential oil and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Example 1 Extraction and component analysis of essential oils of different strains of litsea cubeba in the national distribution area

[0034] 1. Extraction of litsea cubeba essential oil

[0035] Take litsea cubeba whole distribution area Guizhou, Chongqing, Zhejiang, Jiangxi, Guangxi, Fujian, Anhui, Hunan provinces two main distribution areas (city or state or district) respectively, take more than 10 litsea cubeba in each area, collect Ripe fruit in August. Use Clevenger volatile oil extraction device (Clevenger volatile oil extraction device includes a 2000mL round bottom flask, vertical tube, condenser tube, pipette with stopcock and return tube. The return tube connects the bottom of the pipette to the vertical tube, the vertical tube and the condenser The top of the tube is attached.) Separate the litsea cubeba oil. The Clevenger volatile oil extraction apparatus consists of a 2000mL round bottom flask, vertical tube, condenser, pipette with stopcock and reflux...

Embodiment 2

[0046] Example 2 Extraction and Component Analysis of Litsea Cubeba Essential Oil Picked in Different Periods

[0047] For the extraction and component determination and analysis methods of Litsea Cubeba, refer to the corresponding method in Example 1. This example focuses on the antibacterial activity of essential oils of litsea cubeba fruit picked in different periods.

[0048] The fruit essential oil of Litsea Cubeba in Guizhou was selected for analysis in different periods. From June to the end of August, it was divided into four periods, namely June 15 (1#), July 15 (2#), and August 15 (3#) and August 25th (4#), the fruit was collected according to these four periods, the essential oil was extracted, the components were determined, and the antibacterial activity was tested.

[0049] As a result, it was found that the essential oils extracted during period 3 (August 15) and period 4 (August 25) had the strongest antibacterial activity, with EC50 of 2.579ul / L and 2.523ul / L...

Embodiment 3

[0053] Example 3 Antibacterial Activity Experiment of Litsea Cubeba Different Strains Essential Oils in National Distribution Areas

[0054] For the extraction and component determination and analysis methods of Litsea Cubeba, refer to the corresponding method in Example 1. This example focuses on the antibacterial activity of essential oils of litsea cubeba fruit picked in different periods.

[0055] 1. Bacteriostasis experiment setup for essential oil experiments

[0056] According to the above findings, the fruit was collected in the middle and late August, and the essential oil was extracted. Five essential oil concentration gradients were set: 0.0625 μL / mL, 0.125 μL / mL, 0.25 μL / mL, 0.5 μL / mL, 1 μL / mL. First dilute the essential oil with organic solvent dimethyl sulfoxide (DMSO) to two milliliters, filter it with a 0.22 μm microporous membrane oil filter for later use, and then add it to the PDA medium so that the final concentration is as above.

[0057] The antibacteri...

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Abstract

The invention provides litsea cubeba essential oil and a preparation method and application thereof. The preparation method has the advantages that the litsea cubeba essential oil is extracted from specific litsea cubeba strains, the fact that essential oil content in fresh fruits reaches 6.45% is discovered, geranial content in the essential oil is larger than 48.00%, neral content in the essential oil is larger than 40.00%, limonene content in the essential oil is larger than 3.00%, terpilenol content in the essential oil is larger than 0.30%, and the minimum inhibitory concentration MIC ofthe obtained essential oil is 0.63 microliter / mL which is evidently lower than polyoxins MIC (17.5 microliter / mL); the half-maximum effect concentration EC50 of the essential oil is 0.09 microliter / mLwhich is evidently lower than polyoxins EC50 (1.64 microliter / mL), and the antibacterial effect of the obtained essential oil is evidently higher than that of other litsea cubeba essential oil. The prepared litsea cubeba essential oil can be used for preparing food flavor enhancers, preservatives and additives and has promising market application prospect in fields of food, medicine and daily chemical products.

Description

technical field [0001] The invention relates to the technical field of extraction of litsea cubeba essential oil, in particular to a preparation method and application of litsea cubeba essential oil with high antibacterial activity. Background technique [0002] Plant essential oils are usually rich in terpenoids, have antioxidant, antibacterial, insecticidal and other activities, and are widely used in flavors and fragrances, pesticides, pharmaceuticals and other industries. [0003] Litsea cubeba is one of the unique spice plant resources in my country. Litsea cubeba essential oil used in the market is mainly the volatile oil extracted from the peel of litsea cubeba. The whole plant of Litsea cubeba contains essential oil, and the essential oil content of the peel is generally about 3% to 4%. It mainly contains citral, higher alcohols, and organic acids. The main component citral content is as high as 60% to 80%, and the highest can reach 90%. An important raw material f...

Claims

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Application Information

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IPC IPC(8): C11B9/02C11B9/00
CPCC11B9/00C11B9/0003C11B9/0007C11B9/0015C11B9/02
Inventor 陈益存汪阳东高暝吴立文
Owner RES INST OF SUBTROPICAL FORESTRY CHINESE ACAD OF FORESTRY
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