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Electrophysiological patch clamp local perfusion liquid replacing and feeding head

A patch clamp and electrophysiological technology, applied in the field of medicine, can solve the problems of weak connection of joint parts, difficulty in meeting experimental requirements, and inability to detect air bubbles in time, and achieves low requirements for drug properties, not easy to be confused, and small dosing error. Effect

Pending Publication Date: 2019-06-21
XIEHE HOSPITAL ATTACHED TO TONGJI MEDICAL COLLEGE HUAZHONG SCI & TECH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, different ion channels have different dynamic characteristics. Some channels have higher requirements on the material and perfusion speed of the perfusion system. The existing full liquid change or large dead space in the dosing head and non-acid-resistant perfusion systems are difficult to meet the experimental requirements. , and the existing dosing head is expensive, easily damaged, and inconvenient to operate
The dosing head of the prior art has multi-channel input and multi-channel output, which is inconvenient to clamp, the output paths are many and easily confused, the joints are many and exposed, the joints are not firmly connected, easy to be damaged, the operation is cumbersome, the device is opaque, and cannot Timely detection of air bubbles will cause liquid addition errors, not suitable for acids, alcohols, high-temperature liquids, and corrosive liquids

Method used

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  • Electrophysiological patch clamp local perfusion liquid replacing and feeding head
  • Electrophysiological patch clamp local perfusion liquid replacing and feeding head
  • Electrophysiological patch clamp local perfusion liquid replacing and feeding head

Examples

Experimental program
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Effect test

Embodiment 1

[0017] Such as figure 1 , figure 2 and image 3 As shown, the electrophysiological patch clamp local perfusion liquid exchange drug supply head provided by the present invention consists of seven capillaries 1, a glass sleeve 2, a joint protection sleeve 3, seven transition tubes 4 and seven hoses 5 One end of each capillary 1 is connected to one end of each transition tube 4 respectively; the glass sleeve 2 is set on the outside of the capillary bundle composed of seven capillaries 1 gathered together, and the inner wall of the glass sleeve 2 and the capillary bundle The gaps between the capillary tubes and the gaps between the capillary tubes 1 are filled and sealed with epoxy resin AB glue. The end face of the capillary tube 1 exposed at the end of the glass sleeve tube 2 is a polished flat plane without gaps. The glass sleeve at the flat end without gaps The end face of the tube 2 is in the same plane as the end face of the capillary 1. The glass sleeve 2 can make the c...

Embodiment 2

[0019]On the basis of Example 1, the specific specifications of capillary 1, glass sleeve 2, joint protection sleeve 3, transition tube 4 and hose 5 are given: capillary 1 is made of polyimide material with an inner diameter of 0.31 mm, a thin round tubular structure with an outer diameter of 0.36mm; the glass sleeve 2 is a round tubular structure made of borosilicate glass fiber material, and the inner diameter of its cavity is just large enough to accommodate seven capillaries 1; the joint protection sleeve 3 is A circular tubular structure made of polypropylene with an inner diameter of 1.6-2mm and an outer diameter of 3-4mm; the transition tube 4 is a circular tubular structure made of polytetrafluoroethylene with an inner diameter of 0.30mm and an outer diameter of 0.79mm Structure; the flexible tube 5 is a circular tubular structure made of silicone material with an inner diameter of 0.79mm and an outer diameter of 2.2mm, and the rest of the content is the same as that of...

Embodiment 3

[0021] The use of the electrophysiological patch clamp provided by the present invention for local perfusion and fluid replacement.

[0022] Connect the hose end of the electrophysiological patch clamp local perfusion fluid exchange drug feeding head provided by the present invention to the liquid reservoir tube, and the liquid reservoir tube and the liquid reservoir are connected through a two-way valve, and the two-way valve is equipped with a Knob switch can control dosing. Such as Figure 4 As shown, the dosing head is connected with the liquid reservoir 3-9. According to the experimental project, the capillary end is connected to the perfusion tank through a tube or the C tube in the square glass three-row tube produced by Warner (such as Figure 5 shown). Example of usage: Figure 4 To measure the IC of the drug to be tested 50 The schematic diagram of the 3-9 liquid reservoirs are respectively connected to the hose end of the dosing head, and the other two ports of...

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Abstract

The invention provides an electrophysiological patch clamp local perfusion liquid replacing and feeding head, which comprises a plurality of capillary tubes, a glass bushing, a joint protection bushing, a plurality of transition tubes and a plurality of hoses, wherein the numbers of the capillary tubes, the transition tubes and the hoses are the same, one ends of each capillary tube are respectively connected to one ends of the transition tube, the glass bushing is sleeved outside the capillary tube bundle, the end surface of one end, exposed to the glass bushing, of the capillary tube is a flatly-polished and gap-free plane, the joint protection bushing is sleeved outside the joint position of the capillary tube and the transition tube, and one ends of each hose are respectively connectedto one ends of each transition tube. According to the present invention, the electrophysiological patch clamp local perfusion liquid replacing and feeding head has advantages of firmness, durability,convenient operation, multi-channel medicine input, single-channel medicine output, stable flow rate of medicine, low feeding error, high accuracy, low requirements on the properties of medicine, acid resistance, corrosion resistance, high-temperature resistance, wide application range, intuitive liquid feeding, gas bubble generation reducing, experimental error reducing, accurate experimental result and the like, and can be used for determining concentration curves and other kinetic parameters and determine the EC50, the IC50 and the like of medicine.

Description

technical field [0001] The invention belongs to the technical field of medicine, and relates to cell electrophysiology and pharmacology experimental research equipment, in particular to a drug-dosing head for local rapid liquid exchange of a multi-channel perfusion device in electrophysiological patch clamp experiments. Background technique [0002] Electrophysiological technology is a technology that stimulates organisms with various forms of energy (electricity, sound, etc.), measures, records and analyzes bioelectric phenomena and electrical characteristics of organisms, and is the main technology of electrophysiology research. The patch clamp technique is a cell electrophysiology technique that won the Nobel Prize. It draws out the weak bioelectricity by using electrodes, amplifies it through a bioelectricity amplifier, and then displays and records its waveform by an oscilloscope for observation, analysis and preservation. The patch clamp technique reflects the activit...

Claims

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Application Information

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IPC IPC(8): B01L3/02
Inventor 任虹高立敏
Owner XIEHE HOSPITAL ATTACHED TO TONGJI MEDICAL COLLEGE HUAZHONG SCI & TECH UNIV
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