Plant-sourced bactericide for controlling bacterial wilt of tobacco
A plant-derived fungicide, tobacco bacterial wilt technology, applied in the directions of fungicides, plant growth regulators, biocides, etc., can solve the problems of difficult disease-resistant breeding work, unstable control effects, and difficulty in controlling diseases, and achieve easy The effect of large-scale promotion and application, human and animal safety, and low cost
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Embodiment 1
[0025] 1. Experimental equipment
[0026] HG-303 Electric Drying Incubator (Nanjing Experimental Instrument Factory); RE-52A Rotary Concentration Evaporator (Shanghai Yarong Biochemical Instrument Factory); LDZX-30KBS Vertical Pressure Steam Sterilizer (Shanghai Shen'an Medical Instrument Factory) ); SW-CJ-2FD purification bench (Suzhou Antai Air Technology Co., Ltd.); BCD-23O refrigerator (Haier Group); AUW-220 electronic balance (Shimadzu Corporation); Gongnong-16 artificial knapsack sprayer (Provided by the Biosafety Laboratory of Hunan Agricultural University); Mortars, measuring cylinders, beakers, weighing paper, petri dishes, rulers, punches, test tubes, pipettes, plastic basins and other commonly used experimental supplies are available in Hunan Agricultural University Biology Provided by the Academy of Safety Science and Technology.
[0027] 2. Experimental reagents
[0028] 2,3,5-triphenyltetrazolium chloride (TTC is purchased by Sinopharm Group Chemical Reagent Co...
Embodiment 2
[0042] Pot experiment of crude extract of amaranth against tobacco bacterial wilt
[0043] The pot experiment was carried out in the greenhouse of the Plant Hospital of Hunan Agricultural University. First, the cultivated tobacco seedlings were transplanted into plastic pots. After transplanting for 10 days, the stems were punctured to inoculate R. solanacearum. The temperature in the greenhouse was kept at 29-32 ℃, 21-24 ℃ in the evening, and the humidity is 90%, so that the tobacco in different pots can evenly germinate. The pot experiment was divided into 6 treatments: 0.5, 0.25, 0.17, 0.125, 0.1mg / mL amaranth crude extract and 0.82mg / mL streptomycin sulfate treatment, and clear water was used as the blank control. Each treatment required 100 tobacco seedlings. Each treatment was repeated 3 times, and the pesticide was applied at the initial stage of the disease, and the pesticide was applied once every 10 days, and the pesticide was applied for a total of 3 times. Each exp...
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