Plant-sourced bactericide for controlling bacterial wilt of tobacco

A plant-derived fungicide, tobacco bacterial wilt technology, applied in the directions of fungicides, plant growth regulators, biocides, etc., can solve the problems of difficult disease-resistant breeding work, unstable control effects, and difficulty in controlling diseases, and achieve easy The effect of large-scale promotion and application, human and animal safety, and low cost

Inactive Publication Date: 2012-03-28
HUNAN AGRICULTURAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] At present, for this disease, agricultural measures such as paddy and dry crop rotation and lime disinfection during the off-field period are mainly used for prevention and control in production, but the paddy and dry crop rotation is easily restricted by the environment and cultivation conditions, and it is difficult to implement; Although lime can relieve the condition, it is easy to cause soil compaction
Breeding for disease resistance is also facing great difficulties
People try to s

Method used

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  • Plant-sourced bactericide for controlling bacterial wilt of tobacco
  • Plant-sourced bactericide for controlling bacterial wilt of tobacco
  • Plant-sourced bactericide for controlling bacterial wilt of tobacco

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] 1. Experimental equipment

[0026] HG-303 Electric Drying Incubator (Nanjing Experimental Instrument Factory); RE-52A Rotary Concentration Evaporator (Shanghai Yarong Biochemical Instrument Factory); LDZX-30KBS Vertical Pressure Steam Sterilizer (Shanghai Shen'an Medical Instrument Factory) ); SW-CJ-2FD purification bench (Suzhou Antai Air Technology Co., Ltd.); BCD-23O refrigerator (Haier Group); AUW-220 electronic balance (Shimadzu Corporation); Gongnong-16 artificial knapsack sprayer (Provided by the Biosafety Laboratory of Hunan Agricultural University); Mortars, measuring cylinders, beakers, weighing paper, petri dishes, rulers, punches, test tubes, pipettes, plastic basins and other commonly used experimental supplies are available in Hunan Agricultural University Biology Provided by the Academy of Safety Science and Technology.

[0027] 2. Experimental reagents

[0028] 2,3,5-triphenyltetrazolium chloride (TTC is purchased by Sinopharm Group Chemical Reagent Co...

Embodiment 2

[0042] Pot experiment of crude extract of amaranth against tobacco bacterial wilt

[0043] The pot experiment was carried out in the greenhouse of the Plant Hospital of Hunan Agricultural University. First, the cultivated tobacco seedlings were transplanted into plastic pots. After transplanting for 10 days, the stems were punctured to inoculate R. solanacearum. The temperature in the greenhouse was kept at 29-32 ℃, 21-24 ℃ in the evening, and the humidity is 90%, so that the tobacco in different pots can evenly germinate. The pot experiment was divided into 6 treatments: 0.5, 0.25, 0.17, 0.125, 0.1mg / mL amaranth crude extract and 0.82mg / mL streptomycin sulfate treatment, and clear water was used as the blank control. Each treatment required 100 tobacco seedlings. Each treatment was repeated 3 times, and the pesticide was applied at the initial stage of the disease, and the pesticide was applied once every 10 days, and the pesticide was applied for a total of 3 times. Each exp...

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Abstract

The invention belongs to the technical field of agricultural chemicals, particularly relates to the field of plant-sourced bactericides and specifically provides a plant-sourced bactericide for controlling bacterial wilt of tobacco. The main active constituents of the plant-sourced bactericide for controlling bacterial wilt of tobacco are amaranth crude extract and sterile water, wherein each milliliter of the plant-sourced bactericide contains 0.17-0.5 mg of the amaranth crude extract. The plant-sourced bactericide for controlling bacterial wilt of tobacco has the advantages as follows: 1, the raw material of the amaranth crude extract is convenient to obtain and the amaranth crude extract can be produced industrially and can be applied by farmers; 2, the effect of the plant-sourced bactericide is obvious and the median inhibitory concentration EC50 is 0.0415 mg/mL; the effect of the plant-sourced bactericide for controlling bacterial wilt of potted tobacco is 77.00-90.29 percent andis obviously higher than the effects of streptomycin sulphate and other conventional plant-sourced bactericides for controlling bacterial wilt of tobacco; and 3, the amaranth crude extract is high inefficiency, low in toxicity, nuisanceless, and safe to environment.

Description

technical field [0001] The invention belongs to the technical field of pesticides, in particular to the field of botanical fungicides, in particular to a botanical sterilization for preventing and treating tobacco bacterial wilt. Background technique [0002] Amaranth is an annual herb that feeds on tender stems and leaves in the family Amaranthaceae (Amaranthaceae), rich in various nutrients. There are about 40 species of Amaranth in the world, and more than 20 species in my country, which are widely distributed. They are native to the tropical and subtropical regions of Central America and Southeast Asia. China is also one of the origins of Amaranth. There are two main types of amaranth, the vegetable amaranth, in which the leaves are eaten as a vegetable, and the grain amaranth, in which the seeds are harvested, originating in Central or South America. The main species of vegetable amaranth is Amaranthus tricolor, which originated in South Asia or Southeast Asia and grad...

Claims

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Application Information

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IPC IPC(8): A01N65/08A01P3/00
Inventor 廖晓兰张亚苏品黄璜刘双清
Owner HUNAN AGRICULTURAL UNIV
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